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This page was generated on 2026-05-06 11:36 -0400 (Wed, 06 May 2026).

HostnameOSArch (*)R versionInstalled pkgs
nebbiolo1Linux (Ubuntu 24.04.4 LTS)x86_644.6.0 RC (2026-04-17 r89917) -- "Because it was There" 4989
kjohnson3macOS 13.7.7 Venturaarm644.6.0 Patched (2026-04-24 r89963) -- "Because it was There" 4722
Click on any hostname to see more info about the system (e.g. compilers)      (*) as reported by 'uname -p', except on Windows and Mac OS X

Package 1200/2418HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
maaslin3 1.4.0  (landing page)
William Nickols
Snapshot Date: 2026-05-05 13:40 -0400 (Tue, 05 May 2026)
git_url: https://git.bioconductor.org/packages/maaslin3
git_branch: RELEASE_3_23
git_last_commit: f7a411e
git_last_commit_date: 2026-04-28 09:04:13 -0400 (Tue, 28 Apr 2026)
nebbiolo1Linux (Ubuntu 24.04.4 LTS) / x86_64  OK    OK    OK  UNNEEDED, same version is already published
kjohnson3macOS 13.7.7 Ventura / arm64  OK    OK    OK    OK  UNNEEDED, same version is already published
See other builds for maaslin3 in R Universe.


CHECK results for maaslin3 on kjohnson3

To the developers/maintainers of the maaslin3 package:
- Allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/maaslin3.git to reflect on this report. See Troubleshooting Build Report for more information.
- Use the following Renviron settings to reproduce errors and warnings.
- If 'R CMD check' started to fail recently on the Linux builder(s) over a missing dependency, add the missing dependency to 'Suggests:' in your DESCRIPTION file. See Renviron.bioc for more information.

raw results


Summary

Package: maaslin3
Version: 1.4.0
Command: /Library/Frameworks/R.framework/Resources/bin/R CMD check --install=check:maaslin3.install-out.txt --library=/Library/Frameworks/R.framework/Resources/library --no-vignettes --timings maaslin3_1.4.0.tar.gz
StartedAt: 2026-05-05 20:12:08 -0400 (Tue, 05 May 2026)
EndedAt: 2026-05-05 20:14:47 -0400 (Tue, 05 May 2026)
EllapsedTime: 158.9 seconds
RetCode: 0
Status:   OK  
CheckDir: maaslin3.Rcheck
Warnings: 0

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   /Library/Frameworks/R.framework/Resources/bin/R CMD check --install=check:maaslin3.install-out.txt --library=/Library/Frameworks/R.framework/Resources/library --no-vignettes --timings maaslin3_1.4.0.tar.gz
###
##############################################################################
##############################################################################


* using log directory ‘/Users/biocbuild/bbs-3.23-bioc/meat/maaslin3.Rcheck’
* using R version 4.6.0 Patched (2026-04-24 r89963)
* using platform: aarch64-apple-darwin23
* R was compiled by
    Apple clang version 17.0.0 (clang-1700.3.19.1)
    GNU Fortran (GCC) 14.2.0
* running under: macOS Tahoe 26.3.1
* using session charset: UTF-8
* current time: 2026-05-06 00:12:08 UTC
* using option ‘--no-vignettes’
* checking for file ‘maaslin3/DESCRIPTION’ ... OK
* this is package ‘maaslin3’ version ‘1.4.0’
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘maaslin3’ can be installed ... OK
* checking installed package size ... OK
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... NOTE
License stub is invalid DCF.
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking code files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... NOTE
Unexported objects imported by ':::' calls:
  ‘lmerTest:::summary.lmerModLmerTest’ ‘survival:::summary.coxph’
  See the note in ?`:::` about the use of this operator.
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... NOTE
Found the following Rd file(s) with Rd \link{} targets missing package
anchors:
  maaslin3.Rd: mirai
  maaslin_fit.Rd: mirai
  maaslin_log_arguments.Rd: mirai
Please provide package anchors for all Rd \link{} targets not in the
package itself and the base packages.
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... OK
Examples with CPU (user + system) or elapsed time > 5s
                                     user system elapsed
maaslin_plot_results               13.626  0.533  14.263
maaslin_plot_results_from_output   13.376  0.541  13.948
maaslin_contrast_test               6.824  0.685   7.525
maaslin3                            6.769  0.688   7.502
maaslin_write_results               5.726  0.437   6.181
maaslin_write_results_lefse_format  5.634  0.424   6.085
maaslin_fit                         4.780  0.427   5.237
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘testthat.R’
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE

Status: 3 NOTEs
See
  ‘/Users/biocbuild/bbs-3.23-bioc/meat/maaslin3.Rcheck/00check.log’
for details.


Installation output

maaslin3.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   /Library/Frameworks/R.framework/Resources/bin/R CMD INSTALL maaslin3
###
##############################################################################
##############################################################################


* installing to library ‘/Library/Frameworks/R.framework/Versions/4.6/Resources/library’
* installing *source* package ‘maaslin3’ ...
** this is package ‘maaslin3’ version ‘1.4.0’
** using staged installation
** R
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (maaslin3)

Tests output

maaslin3.Rcheck/tests/testthat.Rout


R version 4.6.0 Patched (2026-04-24 r89963) -- "Because it was There"
Copyright (C) 2026 The R Foundation for Statistical Computing
Platform: aarch64-apple-darwin23

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(testthat)
> library(maaslin3)
> 
> test_check("maaslin3")
2026-05-05 20:14:24.58 ERROR::Please select a normalization from the list of available options : TSS, CLR, NONE
2026-05-05 20:14:24.58 ERROR::Please select a transform from the list of available options : LOG, PLOG, NONE
2026-05-05 20:14:24.59 ERROR::Please select a correction method from the list of available options : BH, holm, hochberg, hommel, bonferroni, BY
2026-05-05 20:14:24.60 ERROR::No fixed, group, or
                        ordered effects included in formula.
2026-05-05 20:14:24.61 ERROR::Effect name not found in metadata: d
2026-05-05 20:14:24.61 ERROR::No user formula provided
2026-05-05 20:14:24.62 ERROR::Effect name not found in metadata so not applied to formula as random effect: d
2026-05-05 20:14:24.62 ERROR::No fixed/group/ordered/
                        feature-specific effects provided.
2026-05-05 20:14:25.54 INFO::Writing function arguments to log file
2026-05-05 20:14:25.58 INFO::Verifying options selected are valid
2026-05-05 20:14:27.25 INFO::Writing function arguments to log file
2026-05-05 20:14:27.28 INFO::Verifying options selected are valid
2026-05-05 20:14:27.28 INFO::Determining format of input files
2026-05-05 20:14:27.28 INFO::Input format is data samples as rows and metadata samples as rows
2026-05-05 20:14:27.29 INFO::Running selected normalization method: TSS
2026-05-05 20:14:27.29 INFO::Creating output feature tables folder
2026-05-05 20:14:27.29 INFO::Writing normalized data to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/features/data_norm.tsv
2026-05-05 20:14:27.29 INFO::Filter data based on min abundance, min prevalence, and max prevalence
2026-05-05 20:14:27.29 INFO::Total samples in data: 16
2026-05-05 20:14:27.29 INFO::Min samples required with min abundance for a feature not to be filtered: 0.000000
2026-05-05 20:14:27.30 INFO::Max samples allowed with min abundance for a feature not to be filtered: 16.160000
2026-05-05 20:14:27.30 INFO::Total filtered features: 0
2026-05-05 20:14:27.30 INFO::Filtered feature names from abundance, min prevalence, and max prevalence filtering:
2026-05-05 20:14:27.30 INFO::Total features filtered by non-zero variance filtering: 0
2026-05-05 20:14:27.30 INFO::Filtered feature names from variance filtering:
2026-05-05 20:14:27.30 INFO::Writing filtered data to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/features/filtered_data.tsv
2026-05-05 20:14:27.31 INFO::Running selected transform method: LOG
2026-05-05 20:14:27.31 INFO::Writing normalized, filtered, transformed data to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/features/data_transformed.tsv
2026-05-05 20:14:27.31 INFO::Applying z-score to standardize continuous metadata
2026-05-05 20:14:27.31 INFO::Running the linear model component
2026-05-05 20:14:27.33 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:27.33 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:27.34 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:27.34 INFO::Counting total values for each feature
2026-05-05 20:14:27.34 INFO::Running the logistic model component
2026-05-05 20:14:27.37 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:27.37 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:27.37 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:27.38 INFO::Counting total values for each feature
2026-05-05 20:14:27.38 INFO::Re-running abundances for warn_prevalence
2026-05-05 20:14:27.38 INFO::Running selected normalization method: TSS
2026-05-05 20:14:27.38 INFO::Running selected transform method: LOG
2026-05-05 20:14:27.41 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:27.41 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:27.42 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:27.42 INFO::Creating fits folder
2026-05-05 20:14:27.42 INFO::Writing residuals to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/fits/residuals_linear.rds
2026-05-05 20:14:27.42 INFO::Writing fitted values to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/fits/fitted_linear.rds
2026-05-05 20:14:27.43 INFO::Writing residuals to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/fits/residuals_logistic.rds
2026-05-05 20:14:27.43 INFO::Writing fitted values to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/fits/fitted_logistic.rds
2026-05-05 20:14:27.43 INFO::Writing all the results to file (ordered 
            by increasing individual q-values): /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/all_results.tsv
2026-05-05 20:14:27.43 INFO::Writing the significant results without errors (those which have joint q-values less than or equal to the threshold of 0.100000 ) to file (ordered by increasing individual q-values): /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/significant_results.tsv
2026-05-05 20:14:27.43 INFO::Creating output figures folder
2026-05-05 20:14:27.44 INFO::Writing summary plot of significant
                        results to file: /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/figures/summary_plot.pdf
2026-05-05 20:14:28.05 INFO::Writing association plots (one for each significant association) to output folder: /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/figures
2026-05-05 20:14:28.05 INFO::Plotting associations from most to least significant, grouped by metadata
2026-05-05 20:14:28.05 INFO::Creating boxplot for continuous data (logistic), var1 vs a
2026-05-05 20:14:28.24 INFO::Writing summary plot of
                        significant results to file: /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/figures/summary_plot.pdf
2026-05-05 20:14:28.79 INFO::Writing association plots (one for each significant association) to output folder: /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e15581f68/figures
2026-05-05 20:14:28.79 INFO::Plotting associations from most to least significant, grouped by metadata
2026-05-05 20:14:28.80 INFO::Creating boxplot for continuous data (logistic), var1 vs a
2026-05-05 20:14:28.98 INFO::Running the linear model component
2026-05-05 20:14:28.99 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:28.99 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:29.00 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:29.00 INFO::Counting total values for each feature
2026-05-05 20:14:29.00 INFO::Running the logistic model component
2026-05-05 20:14:29.01 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:29.01 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:29.01 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:29.01 INFO::Counting total values for each feature
2026-05-05 20:14:29.01 INFO::Re-running abundances for warn_prevalence
2026-05-05 20:14:29.02 INFO::Running selected normalization method: TSS
2026-05-05 20:14:29.02 INFO::Running selected transform method: LOG
2026-05-05 20:14:29.02 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:29.03 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:29.03 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:29.03 INFO::Creating output folder
2026-05-05 20:14:29.04 INFO::Creating output figures folder
2026-05-05 20:14:29.04 INFO::Writing summary plot of significant
                        results to file: /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e50453785/figures/summary_plot.pdf
2026-05-05 20:14:29.59 INFO::Writing association plots (one for each significant association) to output folder: /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e50453785/figures
2026-05-05 20:14:29.59 INFO::Plotting associations from most to least significant, grouped by metadata
2026-05-05 20:14:29.59 INFO::Creating boxplot for continuous data (logistic), var1 vs a
2026-05-05 20:14:29.79 INFO::Applying z-score to standardize continuous metadata
2026-05-05 20:14:29.79 INFO::Factor detected for categorial metadata 'c'. Using as-is.
2026-05-05 20:14:29.79 INFO::Bypass z-score application to metadata
2026-05-05 20:14:29.79 INFO::Bypass z-score application to metadata
2026-05-05 20:14:29.81 INFO::Determining format of input files
2026-05-05 20:14:29.81 INFO::Input format is data samples as rows and metadata samples as rows
2026-05-05 20:14:29.81 INFO::Input format is data samples as rows and feature_specific_covariate samples as rows
2026-05-05 20:14:29.81 INFO::Determining format of input files
2026-05-05 20:14:29.82 INFO::Input format is data samples as columns and metadata samples as rows
2026-05-05 20:14:29.82 INFO::Input format is feature_specific_covariate samples as columns
2026-05-05 20:14:29.82 INFO::Determining format of input files
2026-05-05 20:14:29.82 INFO::Input format is data samples as rows and metadata samples as rows
2026-05-05 20:14:29.82 INFO::Input format is feature_specific_covariate samples as columns
2026-05-05 20:14:29.82 INFO::The following samples were found to have metadata but no features (or feature specific covariates if applicable). They will be removed. sample1
2026-05-05 20:14:29.82 INFO::The following samples were found to have feature specific covariates but no features or no metadata. They will be removed. sample1
2026-05-05 20:14:29.83 INFO::Running selected transform method: LOG
2026-05-05 20:14:29.83 INFO::Creating output feature tables folder
2026-05-05 20:14:29.83 INFO::Writing normalized, filtered, transformed data to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e5d0f379c/features/data_transformed.tsv
2026-05-05 20:14:29.83 INFO::Running selected transform method: LOG
2026-05-05 20:14:29.83 INFO::Writing normalized, filtered, transformed data to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e5d0f379c/features/data_transformed.tsv
2026-05-05 20:14:29.84 INFO::Running selected transform method: PLOG
2026-05-05 20:14:29.84 INFO::Writing normalized, filtered, transformed data to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e5d0f379c/features/data_transformed.tsv
2026-05-05 20:14:29.84 INFO::Running selected transform method: NONE
2026-05-05 20:14:29.84 INFO::Writing normalized, filtered, transformed data to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e5d0f379c/features/data_transformed.tsv
2026-05-05 20:14:29.85 INFO::Running the linear model component
2026-05-05 20:14:29.85 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:29.86 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:29.86 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:29.86 INFO::Counting total values for each feature
2026-05-05 20:14:29.86 INFO::Running the logistic model component
2026-05-05 20:14:29.87 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:29.87 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:29.87 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:29.88 INFO::Counting total values for each feature
2026-05-05 20:14:29.88 INFO::Re-running abundances for warn_prevalence
2026-05-05 20:14:29.88 INFO::Running selected normalization method: TSS
2026-05-05 20:14:29.88 INFO::Running selected transform method: LOG
2026-05-05 20:14:29.89 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:29.89 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:29.89 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:29.90 INFO::Creating output folder
2026-05-05 20:14:29.90 INFO::Creating fits folder
2026-05-05 20:14:29.90 INFO::Writing residuals to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e120c127f/fits/residuals_linear.rds
2026-05-05 20:14:29.90 INFO::Writing fitted values to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e120c127f/fits/fitted_linear.rds
2026-05-05 20:14:29.90 INFO::Writing residuals to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e120c127f/fits/residuals_logistic.rds
2026-05-05 20:14:29.90 INFO::Writing fitted values to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e120c127f/fits/fitted_logistic.rds
2026-05-05 20:14:29.90 INFO::Writing all the results to file (ordered 
            by increasing individual q-values): /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e120c127f/all_results.tsv
2026-05-05 20:14:29.91 INFO::Writing the significant results without errors (those which have joint q-values less than or equal to the threshold of 0.100000 ) to file (ordered by increasing individual q-values): /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097e120c127f/significant_results.tsv
2026-05-05 20:14:30.48 INFO::Running the linear model component
2026-05-05 20:14:30.49 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:30.49 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:30.49 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:30.49 INFO::Counting total values for each feature
2026-05-05 20:14:30.49 INFO::Running the logistic model component
2026-05-05 20:14:30.50 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:30.50 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:30.50 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:30.51 INFO::Counting total values for each feature
2026-05-05 20:14:30.51 INFO::Re-running abundances for warn_prevalence
2026-05-05 20:14:30.51 INFO::Running selected normalization method: TSS
2026-05-05 20:14:30.51 INFO::Running selected transform method: LOG
2026-05-05 20:14:30.52 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:30.52 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:30.52 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:31.35 INFO::Started 2 mirai daemons via `cores` argument
2026-05-05 20:14:31.35 INFO::Running the linear model component
2026-05-05 20:14:31.91 INFO::Counting total values for each feature
2026-05-05 20:14:31.91 INFO::Running the logistic model component
2026-05-05 20:14:31.94 INFO::Counting total values for each feature
2026-05-05 20:14:31.95 INFO::Re-running abundances for warn_prevalence
2026-05-05 20:14:31.95 INFO::Running selected normalization method: TSS
2026-05-05 20:14:31.95 INFO::Running selected transform method: LOG
2026-05-05 20:14:33.52 INFO::Running the linear model component
2026-05-05 20:14:34.10 INFO::Counting total values for each feature
2026-05-05 20:14:34.10 INFO::Running the logistic model component
2026-05-05 20:14:34.13 INFO::Counting total values for each feature
2026-05-05 20:14:34.13 INFO::Re-running abundances for warn_prevalence
2026-05-05 20:14:34.14 INFO::Running selected normalization method: TSS
2026-05-05 20:14:34.14 INFO::Running selected transform method: LOG
2026-05-05 20:14:36.21 INFO::Running the linear model component
2026-05-05 20:14:36.78 INFO::Counting total values for each feature
2026-05-05 20:14:36.78 INFO::Running the logistic model component
2026-05-05 20:14:36.81 INFO::Counting total values for each feature
2026-05-05 20:14:36.82 INFO::Re-running abundances for warn_prevalence
2026-05-05 20:14:36.82 INFO::Running selected normalization method: TSS
2026-05-05 20:14:36.82 INFO::Running selected transform method: LOG
2026-05-05 20:14:38.38 INFO::Writing function arguments to log file
2026-05-05 20:14:38.40 INFO::Verifying options selected are valid
2026-05-05 20:14:38.40 INFO::Determining format of input files
2026-05-05 20:14:38.41 INFO::Input format is data samples as rows and metadata samples as rows
2026-05-05 20:14:38.41 INFO::Running selected normalization method: TSS
2026-05-05 20:14:38.42 INFO::Creating output feature tables folder
2026-05-05 20:14:38.42 INFO::Writing normalized data to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/maaslin3_parallel_test_1097e49ffe22b/features/data_norm.tsv
2026-05-05 20:14:38.42 INFO::Filter data based on min abundance, min prevalence, and max prevalence
2026-05-05 20:14:38.42 INFO::Total samples in data: 30
2026-05-05 20:14:38.42 INFO::Min samples required with min abundance for a feature not to be filtered: 0.000000
2026-05-05 20:14:38.43 INFO::Max samples allowed with min abundance for a feature not to be filtered: 30.300000
2026-05-05 20:14:38.43 INFO::Total filtered features: 0
2026-05-05 20:14:38.43 INFO::Filtered feature names from abundance, min prevalence, and max prevalence filtering:
2026-05-05 20:14:38.43 INFO::Total features filtered by non-zero variance filtering: 0
2026-05-05 20:14:38.43 INFO::Filtered feature names from variance filtering:
2026-05-05 20:14:38.43 INFO::Writing filtered data to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/maaslin3_parallel_test_1097e49ffe22b/features/filtered_data.tsv
2026-05-05 20:14:38.44 INFO::Running selected transform method: LOG
2026-05-05 20:14:38.44 INFO::Writing normalized, filtered, transformed data to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/maaslin3_parallel_test_1097e49ffe22b/features/data_transformed.tsv
2026-05-05 20:14:38.44 INFO::Applying z-score to standardize continuous metadata
2026-05-05 20:14:38.44 INFO::Running the linear model component
2026-05-05 20:14:39.02 INFO::Counting total values for each feature
2026-05-05 20:14:39.02 INFO::Running the logistic model component
2026-05-05 20:14:39.08 INFO::Counting total values for each feature
2026-05-05 20:14:39.09 INFO::Creating fits folder
2026-05-05 20:14:39.09 INFO::Writing residuals to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/maaslin3_parallel_test_1097e49ffe22b/fits/residuals_linear.rds
2026-05-05 20:14:39.09 INFO::Writing fitted values to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/maaslin3_parallel_test_1097e49ffe22b/fits/fitted_linear.rds
2026-05-05 20:14:39.09 INFO::Writing residuals to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/maaslin3_parallel_test_1097e49ffe22b/fits/residuals_logistic.rds
2026-05-05 20:14:39.09 INFO::Writing fitted values to file /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/maaslin3_parallel_test_1097e49ffe22b/fits/fitted_logistic.rds
2026-05-05 20:14:39.10 INFO::Writing all the results to file (ordered 
            by increasing individual q-values): /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/maaslin3_parallel_test_1097e49ffe22b/all_results.tsv
2026-05-05 20:14:39.10 INFO::Writing the significant results without errors (those which have joint q-values less than or equal to the threshold of 0.100000 ) to file (ordered by increasing individual q-values): /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/maaslin3_parallel_test_1097e49ffe22b/significant_results.tsv
2026-05-05 20:14:39.83 INFO::Running the linear model component
2026-05-05 20:14:39.84 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:39.84 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:39.84 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:39.84 INFO::Counting total values for each feature
2026-05-05 20:14:39.84 INFO::Running the logistic model component
2026-05-05 20:14:39.85 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:39.85 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:39.85 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:39.86 INFO::Counting total values for each feature
2026-05-05 20:14:39.86 INFO::Re-running abundances for warn_prevalence
2026-05-05 20:14:39.86 INFO::Running selected normalization method: TSS
2026-05-05 20:14:39.86 INFO::Running selected transform method: LOG
2026-05-05 20:14:39.87 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:39.87 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:39.87 INFO::Fitting model to feature number 3, c
2026-05-05 20:14:40.17 INFO::Started 2 mirai daemons via `cores` argument
2026-05-05 20:14:40.17 INFO::Running the linear model component
2026-05-05 20:14:40.72 INFO::Counting total values for each feature
2026-05-05 20:14:40.72 INFO::Running the logistic model component
2026-05-05 20:14:40.75 INFO::Counting total values for each feature
2026-05-05 20:14:40.76 INFO::Re-running abundances for warn_prevalence
2026-05-05 20:14:40.76 INFO::Running selected normalization method: TSS
2026-05-05 20:14:40.76 INFO::Running selected transform method: LOG
2026-05-05 20:14:41.00 INFO::Running the linear model component
2026-05-05 20:14:41.00 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:41.00 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:41.01 INFO::Counting total values for each feature
2026-05-05 20:14:41.01 INFO::Running the logistic model component
2026-05-05 20:14:41.01 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:41.02 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:41.02 INFO::Counting total values for each feature
2026-05-05 20:14:41.02 INFO::Re-running abundances for warn_prevalence
2026-05-05 20:14:41.02 INFO::Running selected normalization method: TSS
2026-05-05 20:14:41.02 INFO::Running selected transform method: LOG
2026-05-05 20:14:41.03 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:41.03 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:41.03 INFO::Creating output folder
2026-05-05 20:14:41.04 INFO::Creating output figures folder
2026-05-05 20:14:41.04 INFO::Writing summary plot of significant
                        results to file: /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097eefb11d9/figures/summary_plot.pdf
2026-05-05 20:14:41.85 INFO::Writing association plots (one for each significant association) to output folder: /var/folders/r0/l4fjk6cj5xj0j3brt4bplpl40000gt/T//RtmpqBjAx0/file1097eefb11d9/figures
2026-05-05 20:14:41.86 INFO::Plotting associations from most to least significant, grouped by metadata
2026-05-05 20:14:41.86 INFO::Creating scatter plot for continuous 
                        data (linear), var1 vs b
2026-05-05 20:14:42.06 INFO::Creating boxplot for continuous data (logistic), var1 vs a
2026-05-05 20:14:42.83 INFO::Running the linear model component
2026-05-05 20:14:42.83 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:42.86 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:42.88 INFO::Counting total values for each feature
2026-05-05 20:14:42.88 INFO::Running the logistic model component
2026-05-05 20:14:42.88 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:42.92 INFO::Fitting model to feature number 2, b
2026-05-05 20:14:42.95 INFO::Counting total values for each feature
2026-05-05 20:14:42.95 INFO::Re-running abundances for warn_prevalence
2026-05-05 20:14:42.95 INFO::Running selected normalization method: TSS
2026-05-05 20:14:42.95 INFO::Running selected transform method: LOG
2026-05-05 20:14:42.96 INFO::Fitting model to feature number 1, a
2026-05-05 20:14:42.98 INFO::Fitting model to feature number 2, b
[ FAIL 0 | WARN 0 | SKIP 1 | PASS 190 ]

══ Skipped tests (1) ═══════════════════════════════════════════════════════════
• empty test (1): 'test_no_dplyr_in_tests.R:4:1'

[ FAIL 0 | WARN 0 | SKIP 1 | PASS 190 ]
> 
> 
> proc.time()
   user  system elapsed 
 58.460   1.586  70.548 

Example timings

maaslin3.Rcheck/maaslin3-Ex.timings

nameusersystemelapsed
maaslin36.7690.6887.502
maaslin_check_arguments0.0530.0020.054
maaslin_check_formula0.0630.0190.082
maaslin_compute_formula0.0650.0230.088
maaslin_contrast_test6.8240.6857.525
maaslin_filter0.2390.0370.277
maaslin_fit4.7800.4275.237
maaslin_log_arguments0.0640.0160.081
maaslin_normalize0.1360.0230.161
maaslin_plot_results13.626 0.53314.263
maaslin_plot_results_from_output13.376 0.54113.948
maaslin_process_metadata0.2460.0560.303
maaslin_read_data0.0640.0180.082
maaslin_reorder_data0.0660.0190.085
maaslin_transform0.3010.0420.345
maaslin_write_results5.7260.4376.181
maaslin_write_results_lefse_format5.6340.4246.085
preprocess_dna_mtx0.0010.0000.002
preprocess_taxa_mtx0.0010.0000.001