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This page was generated on 2025-08-02 12:05 -0400 (Sat, 02 Aug 2025).

HostnameOSArch (*)R versionInstalled pkgs
nebbiolo2Linux (Ubuntu 24.04.2 LTS)x86_644.5.1 (2025-06-13) -- "Great Square Root" 4796
palomino8Windows Server 2022 Datacenterx644.5.1 (2025-06-13 ucrt) -- "Great Square Root" 4535
lconwaymacOS 12.7.1 Montereyx86_644.5.1 (2025-06-13) -- "Great Square Root" 4578
kjohnson3macOS 13.7.1 Venturaarm644.5.1 Patched (2025-06-14 r88325) -- "Great Square Root" 4519
taishanLinux (openEuler 24.03 LTS)aarch644.5.0 (2025-04-11) -- "How About a Twenty-Six" 4517
Click on any hostname to see more info about the system (e.g. compilers)      (*) as reported by 'uname -p', except on Windows and Mac OS X

Package 2060/2313HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
SpliceWiz 1.11.0  (landing page)
Alex Chit Hei Wong
Snapshot Date: 2025-08-01 13:25 -0400 (Fri, 01 Aug 2025)
git_url: https://git.bioconductor.org/packages/SpliceWiz
git_branch: devel
git_last_commit: ff5b222
git_last_commit_date: 2025-04-15 12:53:12 -0400 (Tue, 15 Apr 2025)
nebbiolo2Linux (Ubuntu 24.04.2 LTS) / x86_64  OK    OK    OK  UNNEEDED, same version is already published
palomino8Windows Server 2022 Datacenter / x64  OK    OK    OK    OK  UNNEEDED, same version is already published
lconwaymacOS 12.7.1 Monterey / x86_64  OK    OK    OK    OK  UNNEEDED, same version is already published
kjohnson3macOS 13.7.1 Ventura / arm64  OK    OK    OK    OK  UNNEEDED, same version is already published
taishanLinux (openEuler 24.03 LTS) / aarch64  OK    OK    OK  


CHECK results for SpliceWiz on nebbiolo2

To the developers/maintainers of the SpliceWiz package:
- Allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/SpliceWiz.git to reflect on this report. See Troubleshooting Build Report for more information.
- Use the following Renviron settings to reproduce errors and warnings.
- If 'R CMD check' started to fail recently on the Linux builder(s) over a missing dependency, add the missing dependency to 'Suggests:' in your DESCRIPTION file. See Renviron.bioc for more information.

raw results


Summary

Package: SpliceWiz
Version: 1.11.0
Command: /home/biocbuild/bbs-3.22-bioc/R/bin/R CMD check --install=check:SpliceWiz.install-out.txt --library=/home/biocbuild/bbs-3.22-bioc/R/site-library --timings SpliceWiz_1.11.0.tar.gz
StartedAt: 2025-08-02 03:11:57 -0400 (Sat, 02 Aug 2025)
EndedAt: 2025-08-02 03:30:25 -0400 (Sat, 02 Aug 2025)
EllapsedTime: 1108.2 seconds
RetCode: 0
Status:   OK  
CheckDir: SpliceWiz.Rcheck
Warnings: 0

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.22-bioc/R/bin/R CMD check --install=check:SpliceWiz.install-out.txt --library=/home/biocbuild/bbs-3.22-bioc/R/site-library --timings SpliceWiz_1.11.0.tar.gz
###
##############################################################################
##############################################################################


* using log directory ‘/home/biocbuild/bbs-3.22-bioc/meat/SpliceWiz.Rcheck’
* using R version 4.5.1 (2025-06-13)
* using platform: x86_64-pc-linux-gnu
* R was compiled by
    gcc (Ubuntu 13.3.0-6ubuntu2~24.04) 13.3.0
    GNU Fortran (Ubuntu 13.3.0-6ubuntu2~24.04) 13.3.0
* running under: Ubuntu 24.04.2 LTS
* using session charset: UTF-8
* checking for file ‘SpliceWiz/DESCRIPTION’ ... OK
* this is package ‘SpliceWiz’ version ‘1.11.0’
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... INFO
Imports includes 44 non-default packages.
Importing from so many packages makes the package vulnerable to any of
them becoming unavailable.  Move as many as possible to Suggests and
use conditionally.
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘SpliceWiz’ can be installed ... OK
* used C++ compiler: ‘g++ (Ubuntu 13.3.0-6ubuntu2~24.04) 13.3.0’
* checking C++ specification ... NOTE
  Specified C++11: please drop specification unless essential
* checking installed package size ... INFO
  installed size is 12.3Mb
  sub-directories of 1Mb or more:
    R      2.2Mb
    libs   8.8Mb
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... NOTE
License stub is invalid DCF.
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking code files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking loading without being on the library search path ... OK
* checking whether startup messages can be suppressed ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... NOTE
Non-topic package-anchored link(s) in Rd file 'ASE-methods.Rd':
  ‘[limma:toptable]{limma::topTable}’

See section 'Cross-references' in the 'Writing R Extensions' manual.

Found the following Rd file(s) with Rd \link{} targets missing package
anchors:
  NxtSE-class.Rd: SummarizedExperiment-class
  SpliceWiz-package.Rd: SummarizedExperiment-class, rowData, colData
  makeSE.Rd: colData
Please provide package anchors for all Rd \link{} targets not in the
package itself and the base packages.
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking line endings in shell scripts ... OK
* checking line endings in C/C++/Fortran sources/headers ... OK
* checking line endings in Makefiles ... OK
* checking compilation flags in Makevars ... OK
* checking for GNU extensions in Makefiles ... OK
* checking for portable use of $(BLAS_LIBS) and $(LAPACK_LIBS) ... OK
* checking use of PKG_*FLAGS in Makefiles ... OK
* checking compiled code ... NOTE
Note: information on .o files is not available
* checking files in ‘vignettes’ ... OK
* checking examples ... OK
Examples with CPU (user + system) or elapsed time > 5s
                          user system elapsed
Build-Reference-methods 52.527  1.448  54.475
NxtSE-class             51.341  1.977  53.342
ASE-methods             28.983  1.523  30.506
covPlotObject-class     19.706  0.885  20.312
plotCoverage            13.319  0.413  13.646
Run_SpliceWiz_Filters    7.860  0.229   8.089
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘testthat.R’
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes ... OK
* checking re-building of vignette outputs ... OK
* checking PDF version of manual ... OK
* DONE

Status: 4 NOTEs
See
  ‘/home/biocbuild/bbs-3.22-bioc/meat/SpliceWiz.Rcheck/00check.log’
for details.


Installation output

SpliceWiz.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.22-bioc/R/bin/R CMD INSTALL SpliceWiz
###
##############################################################################
##############################################################################


* installing to library ‘/home/biocbuild/bbs-3.22-bioc/R/site-library’
* installing *source* package ‘SpliceWiz’ ...
** this is package ‘SpliceWiz’ version ‘1.11.0’
** using staged installation
** libs
using C++ compiler: ‘g++ (Ubuntu 13.3.0-6ubuntu2~24.04) 13.3.0’
using C++11
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c BAM2blocks.cpp -o BAM2blocks.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c FastaReader.cpp -o FastaReader.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c FragmentBlocks.cpp -o FragmentBlocks.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c GZTools.cpp -o GZTools.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c RcppExports.cpp -o RcppExports.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c ReadBlockProcessor.cpp -o ReadBlockProcessor.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c ReadBlockProcessor_CoverageBlocks.cpp -o ReadBlockProcessor_CoverageBlocks.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c ReadBlockProcessor_FragmentsMap.cpp -o ReadBlockProcessor_FragmentsMap.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c ReadBlockProcessor_TandemJunctions.cpp -o ReadBlockProcessor_TandemJunctions.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c covTools.cpp -o covTools.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c main.cpp -o main.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c swEngine.cpp -o swEngine.o
g++ -std=gnu++11 -I"/home/biocbuild/bbs-3.22-bioc/R/include" -DNDEBUG  -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/ompBAM/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/Rcpp/include' -I'/home/biocbuild/bbs-3.22-bioc/R/site-library/RcppProgress/include' -I/usr/local/include   -fopenmp -DSPLICEWIZ -fpic  -g -O2  -Wall -Werror=format-security  -c synthReadGenerator.cpp -o synthReadGenerator.o
g++ -std=gnu++11 -shared -L/home/biocbuild/bbs-3.22-bioc/R/lib -L/usr/local/lib -o SpliceWiz.so BAM2blocks.o FastaReader.o FragmentBlocks.o GZTools.o RcppExports.o ReadBlockProcessor.o ReadBlockProcessor_CoverageBlocks.o ReadBlockProcessor_FragmentsMap.o ReadBlockProcessor_TandemJunctions.o covTools.o main.o swEngine.o synthReadGenerator.o -fopenmp -DSPLICEWIZ -L/home/biocbuild/bbs-3.22-bioc/R/lib -lR
installing to /home/biocbuild/bbs-3.22-bioc/R/site-library/00LOCK-SpliceWiz/00new/SpliceWiz/libs
** R
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** checking absolute paths in shared objects and dynamic libraries
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (SpliceWiz)

Tests output

SpliceWiz.Rcheck/tests/testthat.Rout


R version 4.5.1 (2025-06-13) -- "Great Square Root"
Copyright (C) 2025 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(testthat)
> library(SpliceWiz)
Loading required package: NxtIRFdata
SpliceWiz package loaded with 2 threads
Use setSWthreads() to set the number of SpliceWiz threads
> 
> test_check("SpliceWiz")
Loading required package: Rsubread
Aug 02 03:21:19 Converting FASTA to local TwoBitFile...done
Aug 02 03:21:20 Connecting to genome TwoBitFile...done
Aug 02 03:21:20 Making local copy of GTF file...done
Aug 02 03:21:20 Extracting temp genome FASTA from TwoBit file
Aug 02 03:21:21 Generating synthetic reads, saving to /tmp/RtmpurVoF1/refWithMapExcl/Mappability/Reads.fa
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
10145 synthetic reads generated
Aug 02 03:21:21 Cleaning temp genome / gene annotation files

        ==========     _____ _    _ ____  _____  ______          _____  
        =====         / ____| |  | |  _ \|  __ \|  ____|   /\   |  __ \ 
          =====      | (___ | |  | | |_) | |__) | |__     /  \  | |  | |
            ====      \___ \| |  | |  _ <|  _  /|  __|   / /\ \ | |  | |
              ====    ____) | |__| | |_) | | \ \| |____ / ____ \| |__| |
        ==========   |_____/ \____/|____/|_|  \_\______/_/    \_\_____/
       Rsubread 2.23.3

//================================= setting ==================================\\
||                                                                            ||
||                Index name : reference_index                                ||
||               Index space : base space                                     ||
||               Index split : no-split                                       ||
||          Repeat threshold : 100 repeats                                    ||
||              Gapped index : no                                             ||
||                                                                            ||
||       Free / total memory : 75.5GB / 125.4GB                               ||
||                                                                            ||
||               Input files : 1 file in total                                ||
||                             o genome.fa                                    ||
||                                                                            ||
\\============================================================================//

//================================= Running ==================================\\
||                                                                            ||
|| Check the integrity of provided reference sequences ...                    ||
|| No format issues were found                                                ||
|| Scan uninformative subreads in reference sequences ...                     ||
|| Estimate the index size...                                                 ||
||    8%,   0 mins elapsed, rate=39.9k bps/s                                  ||
||   16%,   0 mins elapsed, rate=78.6k bps/s                                  ||
||   24%,   0 mins elapsed, rate=116.4k bps/s                                 ||
||   33%,   0 mins elapsed, rate=153.2k bps/s                                 ||
||   41%,   0 mins elapsed, rate=189.2k bps/s                                 ||
||   49%,   0 mins elapsed, rate=224.6k bps/s                                 ||
||   58%,   0 mins elapsed, rate=259.1k bps/s                                 ||
||   66%,   0 mins elapsed, rate=293.0k bps/s                                 ||
||   74%,   0 mins elapsed, rate=326.4k bps/s                                 ||
||   83%,   0 mins elapsed, rate=359.7k bps/s                                 ||
||   91%,   0 mins elapsed, rate=392.3k bps/s                                 ||
|| 3.0 GB of memory is needed for index building.                             ||
|| Build the index...                                                         ||
||    8%,   0 mins elapsed, rate=4.1k bps/s                                   ||
||   16%,   0 mins elapsed, rate=8.2k bps/s                                   ||
||   24%,   0 mins elapsed, rate=12.3k bps/s                                  ||
||   33%,   0 mins elapsed, rate=16.3k bps/s                                  ||
||   41%,   0 mins elapsed, rate=20.4k bps/s                                  ||
||   49%,   0 mins elapsed, rate=24.4k bps/s                                  ||
||   58%,   0 mins elapsed, rate=28.4k bps/s                                  ||
||   66%,   0 mins elapsed, rate=32.4k bps/s                                  ||
||   74%,   0 mins elapsed, rate=36.4k bps/s                                  ||
||   83%,   0 mins elapsed, rate=40.4k bps/s                                  ||
||   91%,   0 mins elapsed, rate=44.3k bps/s                                  ||
|| Save current index block...                                                ||
||  [ 0.0% finished ]                                                         ||
||  [ 10.0% finished ]                                                        ||
||  [ 20.0% finished ]                                                        ||
||  [ 30.0% finished ]                                                        ||
||  [ 40.0% finished ]                                                        ||
||  [ 50.0% finished ]                                                        ||
||  [ 60.0% finished ]                                                        ||
||  [ 70.0% finished ]                                                        ||
||  [ 80.0% finished ]                                                        ||
||  [ 90.0% finished ]                                                        ||
||  [ 100.0% finished ]                                                       ||
||                                                                            ||
||                      Total running time: 0.2 minutes.                      ||
||              Index ./reference_index was successfully built.               ||
||                                                                            ||
\\============================================================================//


        ==========     _____ _    _ ____  _____  ______          _____  
        =====         / ____| |  | |  _ \|  __ \|  ____|   /\   |  __ \ 
          =====      | (___ | |  | | |_) | |__) | |__     /  \  | |  | |
            ====      \___ \| |  | |  _ <|  _  /|  __|   / /\ \ | |  | |
              ====    ____) | |__| | |_) | | \ \| |____ / ____ \| |__| |
        ==========   |_____/ \____/|____/|_|  \_\______/_/    \_\_____/
       Rsubread 2.23.3

//================================= setting ==================================\\
||                                                                            ||
|| Function      : Read alignment + Junction detection (RNA-Seq)              ||
|| Input file    : Reads.fa                                                   ||
|| Output file   : AlignedReads.bam (BAM)                                     ||
|| Index name    : reference_index                                            ||
||                                                                            ||
||                    ------------------------------------                    ||
||                                                                            ||
||                               Threads : 1                                  ||
||                          Phred offset : 33                                 ||
||                             Min votes : 1 / 14                             ||
||                        Max mismatches : 3                                  ||
||                      Max indel length : 5                                  ||
||            Report multi-mapping reads : yes                                ||
|| Max alignments per multi-mapping read : 1                                  ||
||                           Annotations : transcripts.gtf (GTF)              ||
||                                                                            ||
\\============================================================================//

//=============== Running (02-Aug-2025 03:21:32, pid=1769859) ================\\
||                                                                            ||
|| Check the input reads.                                                     ||
|| The input file contains base space reads.                                  ||
|| Initialise the memory objects.                                             ||
|| Estimate the mean read length.                                             ||
|| Create the output BAM file.                                                ||
|| Check the index.                                                           ||
|| Init the voting space.                                                     ||
|| Load the annotation file.                                                  ||
|| 538 annotation records were loaded.                                        ||
|| Global environment is initialised.                                         ||
|| Load the 1-th index block...                                               ||
|| The index block has been loaded.                                           ||
|| Start read mapping in chunk.                                               ||
||   65% completed, 0.6 mins elapsed, rate=44.4k reads per second             ||
||   83% completed, 0.6 mins elapsed, rate=0.2k reads per second              ||
||   99% completed, 0.6 mins elapsed, rate=0.3k reads per second              ||
||                                                                            ||
||                           Completed successfully.                          ||
||                                                                            ||
\\====================================    ====================================//

//================================   Summary =================================\\
||                                                                            ||
||                 Total reads : 10,145                                       ||
||                      Mapped : 10,145 (100.0%)                              ||
||             Uniquely mapped : 10,145                                       ||
||               Multi-mapping : 0                                            ||
||                                                                            ||
||                    Unmapped : 0                                            ||
||                                                                            ||
||                   Junctions : 0                                            ||
||                      Indels : 1                                            ||
||                                                                            ||
||                Running time : 0.6 minutes                                  ||
||                                                                            ||
\\============================================================================//

Aug 02 03:22:08 Calculating Mappability Exclusion regions from: /tmp/RtmpurVoF1/refWithMapExcl/Mappability/AlignedReads.bam
Calculating Mappability Exclusions: /tmp/RtmpurVoF1/refWithMapExcl/Mappability/AlignedReads.bam
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Performing final sort of fragment maps
Writing Mappability Exclusions
**************************************************|
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Aug 02 03:22:12 Reference generated without non-polyA reference
Aug 02 03:22:12 Reference generated without Mappability reference
Aug 02 03:22:12 Reference generated without Blacklist exclusion
Aug 02 03:22:12 Converting FASTA to local TwoBitFile...done
Aug 02 03:22:13 Connecting to genome TwoBitFile...done
Aug 02 03:22:13 Making local copy of GTF file...done
Aug 02 03:22:13 Reading source GTF file...done
Aug 02 03:22:13 Processing gtf file...
...genes
...transcripts
...CDS
...exons
done
Aug 02 03:22:14 Gene ontology not prepared for this reference
Aug 02 03:22:17 Processing introns...
...data
...basic annotations
...splice motifs
...other info
...defining flanking exon clusters
done
Aug 02 03:22:21 Generating processBAM reference
...prepping data
...determining measurable introns (directional)
...determining measurable introns (non-directional)
...writing ref-cover.bed
...writing ref-ROI.bed
...writing ref-read-continues.ref
...writing ref-sj.ref
...writing ref-tj.ref
processBAM reference generated
Aug 02 03:22:29 Predicting NMD transcripts from genome sequence
...exonic transcripts
...retained introns

  |                                                                            
  |                                                                      |   0%
  |                                                                            
  |======================================================================| 100%
done
Aug 02 03:22:31 Annotating Splice Events
Annotating Mutually-Exclusive-Exon Splice Events...done
Annotating Skipped-Exon Splice Events...done
Annotating Alternate 5' / 3' Splice Site Splice Events...done
Annotating Alternate First / Last Exon Splice Events...done
Annotating known retained introns...done
Aug 02 03:22:32 Splice Annotations Filtered
Aug 02 03:22:33 Translating Alternate Splice Peptides...done
Aug 02 03:22:34 Splice Annotations finished

Reference build finished
Aug 02 03:22:35 Running SpliceWiz processBAM
Reading reference file
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (0 milliseconds)
Processing BAM file /tmp/RtmpurVoF1/02H003.bam
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Performing final sort of fragment maps
Writing COV file
**************************************************|
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Writing output file
/tmp/RtmpurVoF1/02H003.bam processed (428 milliseconds)
Aug 02 03:22:35 Running SpliceWiz processBAM
Reading reference file
Allocating memory to 2 threads for SpliceWiz (ompBAM)...initialized (0 milliseconds)
Processing BAM file /tmp/RtmpurVoF1/02H003.bam
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Compiling data from threads
Performing final sort of fragment maps
Writing COV file
**************************************************|
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Writing output file
/tmp/RtmpurVoF1/02H003.bam processed (236 milliseconds)
Aug 02 03:22:36 Reference generated without non-polyA reference
Aug 02 03:22:36 Reference generated without Mappability reference
Aug 02 03:22:36 Reference generated without Blacklist exclusion
Aug 02 03:22:36 Converting FASTA to local TwoBitFile...done
Aug 02 03:22:37 Connecting to genome TwoBitFile...done
Aug 02 03:22:37 Making local copy of GTF file...done
Aug 02 03:22:37 Reading source GTF file...done
Aug 02 03:22:37 Processing gtf file...
...genes
...transcripts
...CDS
Aug 02 03:22:37 No protein information detected in reference! For full functionality, ensure there are valid entries with type == `CDS` in the gtf file. Protein reference and NMD annotation is skipped.
...exons
done
Aug 02 03:22:38 Gene ontology not prepared for this reference
Aug 02 03:22:40 Processing introns...
...data
...basic annotations
...splice motifs
...other info
...defining flanking exon clusters
done
Aug 02 03:22:44 Generating processBAM reference
...prepping data
...determining measurable introns (directional)
...determining measurable introns (non-directional)
...writing ref-cover.bed
...writing ref-ROI.bed
...writing ref-read-continues.ref
...writing ref-sj.ref
...writing ref-tj.ref
processBAM reference generated
Aug 02 03:22:50 Annotating Splice Events
Annotating Mutually-Exclusive-Exon Splice Events...done
Annotating Skipped-Exon Splice Events...done
Annotating Alternate 5' / 3' Splice Site Splice Events...done
Annotating Alternate First / Last Exon Splice Events...done
Annotating known retained introns...done
Aug 02 03:22:52 Splice Annotations Filtered
Reference build finished
Aug 02 03:22:54 Running SpliceWiz processBAM
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (0 milliseconds)
/tmp/RtmpurVoF1/02H003.bam processed (364 milliseconds)
/tmp/RtmpurVoF1/02H025.bam processed (290 milliseconds)
/tmp/RtmpurVoF1/02H026.bam processed (448 milliseconds)
/tmp/RtmpurVoF1/02H033.bam processed (460 milliseconds)
/tmp/RtmpurVoF1/02H043.bam processed (284 milliseconds)
/tmp/RtmpurVoF1/02H046.bam processed (410 milliseconds)
Aug 02 03:22:57 Using MulticoreParam 1 threads
Aug 02 03:22:57 Validating Experiment; checking COV files...
Aug 02 03:22:57 Compiling Sample Stats
Aug 02 03:22:57 Compiling Junction List...merging...done
Aug 02 03:22:58 Compiling Junction Stats...merging...done
Aug 02 03:23:05 Compiling Intron Retention List...done
Aug 02 03:23:06 Tidying up splice junctions and intron retentions...
...annotating splice junctions
...copying splicing reference
...grouping splice junctions
...grouping introns
...loading splice events
...compiling rowEvents
done

Aug 02 03:23:17 Generating NxtSE assays
Aug 02 03:23:18 Using MulticoreParam 1 threads

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  |======================================================================| 100%

Aug 02 03:23:33 Building Final NxtSE Object
Aug 02 03:23:33 ...consolidating assays to H5 file
Aug 02 03:23:35 ...packaging reference
Aug 02 03:23:35 ...synthesising NxtSE
Aug 02 03:23:35 ...determining how overlapping introns should be removed
Aug 02 03:23:38 SpliceWiz (NxtSE) Collation Finished
Aug 02 03:23:38 Loading NxtSE object from file...
Aug 02 03:23:38 ...loading reference (NxtSE)
Aug 02 03:23:38 ...linking assays
Aug 02 03:23:38 ...linking COV files
Aug 02 03:23:38 ...loading rowData
Aug 02 03:23:38 ...removing overlapping introns...
Aug 02 03:23:38 NxtSE loaded
Aug 02 03:23:38 SpliceWiz reference already exists in given directory
Aug 02 03:23:38 Running SpliceWiz processBAM
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (0 milliseconds)
/tmp/RtmpurVoF1/02H003.bam processed (362 milliseconds)
/tmp/RtmpurVoF1/02H025.bam processed (297 milliseconds)
/tmp/RtmpurVoF1/02H026.bam processed (476 milliseconds)
/tmp/RtmpurVoF1/02H033.bam processed (468 milliseconds)
/tmp/RtmpurVoF1/02H043.bam processed (286 milliseconds)
/tmp/RtmpurVoF1/02H046.bam processed (418 milliseconds)
Aug 02 03:23:42 Using MulticoreParam 1 threads
Aug 02 03:23:42 Validating Experiment; checking COV files...
Aug 02 03:23:42 Compiling Sample Stats
Aug 02 03:23:42 Compiling Junction List...merging...done
Aug 02 03:23:44 Compiling Junction Stats...merging...done
Aug 02 03:23:52 Compiling Intron Retention List...done
Aug 02 03:23:53 Compiling Tandem Junction List...merging...done
Aug 02 03:23:54 Tidying up splice junctions and intron retentions...
...annotating splice junctions
...looking for novel exons
Aug 02 03:23:57 Assembling novel splicing reference:
...loading reference FASTA/GTF
...injecting novel transcripts to GTF
...processing GTF
...processing introns from GTF
...annotating alternative splicing events
done
Aug 02 03:24:07 Tidying up splice junctions and intron retentions (part 2)...
...grouping splice junctions
...grouping introns
...loading splice events
...compiling rowEvents
done

Aug 02 03:24:16 Generating NxtSE assays
Aug 02 03:24:17 Using MulticoreParam 1 threads

  |                                                                            
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  |============                                                          |  17%
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  |=======================                                               |  33%
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Aug 02 03:24:33 Building Final NxtSE Object
Aug 02 03:24:33 ...consolidating assays to H5 file
Aug 02 03:24:34 ...packaging reference
Aug 02 03:24:34 ...synthesising NxtSE
Aug 02 03:24:34 ...determining how overlapping introns should be removed
Aug 02 03:24:37 SpliceWiz (NxtSE) Collation Finished
Aug 02 03:24:37 Loading NxtSE object from file...
Aug 02 03:24:37 ...loading reference (NxtSE)
Aug 02 03:24:37 ...linking assays
Aug 02 03:24:37 ...linking COV files
Aug 02 03:24:37 ...loading rowData
Aug 02 03:24:37 ...removing overlapping introns...
Aug 02 03:24:38 NxtSE loaded
Aug 02 03:24:38 SpliceWiz reference already exists in given directory
Aug 02 03:24:38 Running SpliceWiz processBAM
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (0 milliseconds)
/tmp/RtmpurVoF1/02H003.bam processed (378 milliseconds)
/tmp/RtmpurVoF1/02H025.bam processed (291 milliseconds)
/tmp/RtmpurVoF1/02H026.bam processed (450 milliseconds)
/tmp/RtmpurVoF1/02H033.bam processed (453 milliseconds)
/tmp/RtmpurVoF1/02H043.bam processed (294 milliseconds)
/tmp/RtmpurVoF1/02H046.bam processed (408 milliseconds)
Aug 02 03:24:41 Using MulticoreParam 1 threads
Aug 02 03:24:41 Validating Experiment; checking COV files...
Aug 02 03:24:41 Compiling Sample Stats
Aug 02 03:24:41 Compiling Junction List...merging...done
Aug 02 03:24:43 Compiling Junction Stats...merging...done
Aug 02 03:24:50 Compiling Intron Retention List...done
Aug 02 03:24:51 Tidying up splice junctions and intron retentions...
...annotating splice junctions
...copying splicing reference
...grouping splice junctions
...grouping introns
...loading splice events
...compiling rowEvents
done

Aug 02 03:25:02 Generating NxtSE assays
Aug 02 03:25:04 Using MulticoreParam 1 threads

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  |=======================                                               |  33%
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  |===============================================                       |  67%
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Aug 02 03:25:19 Building Final NxtSE Object
Aug 02 03:25:19 ...consolidating assays to H5 file
Aug 02 03:25:21 ...packaging reference
Aug 02 03:25:21 ...synthesising NxtSE
Aug 02 03:25:21 ...determining how overlapping introns should be removed
Aug 02 03:25:24 SpliceWiz (NxtSE) Collation Finished
Aug 02 03:25:24 Loading NxtSE object from file...
Aug 02 03:25:24 ...loading reference (NxtSE)
Aug 02 03:25:24 ...linking assays
Aug 02 03:25:24 ...linking COV files
Aug 02 03:25:24 ...loading rowData
Aug 02 03:25:24 ...removing overlapping introns...
Aug 02 03:25:24 NxtSE loaded
                                    path paired strand  depth mean_frag_size
02H003 ../SpliceWiz_Output/02H003.txt.gz   TRUE     -1 106256       149.3108
02H025 ../SpliceWiz_Output/02H025.txt.gz   TRUE     -1  84872       151.5914
02H026 ../SpliceWiz_Output/02H026.txt.gz   TRUE     -1 131514       150.3238
02H033 ../SpliceWiz_Output/02H033.txt.gz   TRUE     -1 129013       153.9754
02H043 ../SpliceWiz_Output/02H043.txt.gz   TRUE     -1  80254       155.4004
02H046 ../SpliceWiz_Output/02H046.txt.gz   TRUE     -1 120174       151.2840
       directionality_strength Intergenic_Fraction rRNA_Fraction
02H003                    9850                   0             0
02H025                    9848                   0             0
02H026                    9848                   0             0
02H033                    9855                   0             0
02H043                    9841                   0             0
02H046                    9857                   0             0
       NonPolyA_Fraction Mitochondrial_Fraction Unanno_Jn_Fraction
02H003                 0                      0        0.013449472
02H025                 0                      0        0.011203515
02H026                 0                      0        0.007282382
02H033                 0                      0        0.006310241
02H043                 0                      0        0.005871040
02H046                 0                      0        0.009819533
       NMD_Jn_Fraction Fraction_Splice_Reads Fraction_Span_Reads IRBurden_clean
02H003     0.019509970             0.3506908           0.1735243     0.05796583
02H025     0.019360162             0.3712414           0.1591691     0.03963531
02H026     0.004298323             0.3980261           0.1615037     0.02576549
02H033     0.007705562             0.4003550           0.1734089     0.03090263
02H043     0.028069104             0.3671219           0.1807511     0.03762138
02H046     0.010542303             0.3725598           0.1603508     0.05099352
       IRBurden_exitrons IRBurden_clean_unstranded IRBurden_exitrons_unstranded
02H003         0.1291810                0.03844293                    0.1296020
02H025         0.1192351                0.02730399                    0.1194005
02H026         0.1231055                0.02010410                    0.1231939
02H033         0.1378958                0.02300500                    0.1380215
02H043         0.1392935                0.02286515                    0.1397365
02H046         0.1132136                0.03359077                    0.1133852
       IRBurden_antisense
02H003        0.021393335
02H025        0.013191532
02H026        0.006007119
02H033        0.008453742
02H043        0.015837096
02H046        0.019043703

MD5 is fcdc53e0f7fbd843529077ebd26dd058
Aug 02 03:25:27 Reference generated without non-polyA reference
Aug 02 03:25:27 Reference generated without Mappability reference
Aug 02 03:25:27 Reference generated without Blacklist exclusion
Aug 02 03:25:27 Converting FASTA to local TwoBitFile...done
Aug 02 03:25:28 Connecting to genome TwoBitFile...done
Aug 02 03:25:28 Making local copy of GTF file...done
Aug 02 03:25:28 Reading source GTF file...done
Aug 02 03:25:28 Processing gtf file...
...genes
...transcripts
...CDS
...exons
done
Aug 02 03:25:29 Gene ontology not prepared for this reference
Aug 02 03:25:31 Processing introns...
...data
...basic annotations
...splice motifs
...other info
...defining flanking exon clusters
done
Aug 02 03:25:35 Generating processBAM reference
...prepping data
...determining measurable introns (directional)
...determining measurable introns (non-directional)
...writing ref-cover.bed
...writing ref-ROI.bed
...writing ref-read-continues.ref
...writing ref-sj.ref
...writing ref-tj.ref
processBAM reference generated
Aug 02 03:25:42 Predicting NMD transcripts from genome sequence
...exonic transcripts
...retained introns

  |                                                                            
  |                                                                      |   0%
  |                                                                            
  |======================================================================| 100%
done
Aug 02 03:25:44 Annotating Splice Events
Annotating Mutually-Exclusive-Exon Splice Events...done
Annotating Skipped-Exon Splice Events...done
Annotating Alternate 5' / 3' Splice Site Splice Events...done
Annotating Alternate First / Last Exon Splice Events...done
Annotating known retained introns...done
Aug 02 03:25:45 Splice Annotations Filtered
Aug 02 03:25:46 Translating Alternate Splice Peptides...done
Aug 02 03:25:47 Splice Annotations finished

Reference build finished
[ FAIL 0 | WARN 0 | SKIP 0 | PASS 47 ]
> 
> # bump to trigger r cmd check
> 
> proc.time()
   user  system elapsed 
255.235  29.892 284.994 

Example timings

SpliceWiz.Rcheck/SpliceWiz-Ex.timings

nameusersystemelapsed
ASE-GLM-edgeR3.1570.2023.358
ASE-methods28.983 1.52330.506
ASEFilter-class0.0430.0060.049
Build-Reference-methods52.527 1.44854.475
Coverage0.8920.0090.893
Gene-ontology-methods3.2030.3143.477
Graphics-User-Interface000
Mappability-methods2.7200.3113.031
NxtSE-class51.341 1.97753.342
Run_SpliceWiz_Filters7.8600.2298.089
STAR-methods0.0030.0020.004
View-Reference-methods0.1960.0220.218
collateData3.5720.0503.619
coord2GR0.0240.0010.024
covDataObject-class1.6490.1221.766
covPlotObject-class19.706 0.88520.312
covPlotly-class1.0160.0191.020
example-SpliceWiz-data0.0100.0020.012
findSamples0.0040.0010.005
isCOV0.0110.0000.011
makeSE1.8920.1051.995
make_plot_data0.0420.0000.042
plotCoverage13.319 0.41313.646
processBAM2.2040.2391.415
setSWthreads0.0020.0040.005
theme_white0.1590.0130.171