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### Running command:
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###   /Library/Frameworks/R.framework/Resources/bin/R CMD check --install=check:DMRcate.install-out.txt --library=/Library/Frameworks/R.framework/Resources/library --no-vignettes --timings DMRcate_3.7.0.tar.gz
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* using log directory ‘/Users/biocbuild/bbs-3.23-bioc/meat/DMRcate.Rcheck’
* using R Under development (unstable) (2026-01-15 r89304)
* using platform: aarch64-apple-darwin20
* R was compiled by
    Apple clang version 16.0.0 (clang-1600.0.26.6)
    GNU Fortran (GCC) 14.2.0
* running under: macOS Sonoma 14.8.3
* using session charset: UTF-8
* using option ‘--no-vignettes’
* checking for file ‘DMRcate/DESCRIPTION’ ... OK
* this is package ‘DMRcate’ version ‘3.7.0’
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘DMRcate’ can be installed ... OK
* checking installed package size ... OK
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking code files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking sizes of PDF files under ‘inst/doc’ ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... ERROR
Running examples in ‘DMRcate-Ex.R’ failed
The error most likely occurred in:

> base::assign(".ptime", proc.time(), pos = "CheckExEnv")
> ### Name: DMRcate-package
> ### Title: DMR calling from bisulfite sequencing and Illumina array data
> ### Aliases: DMRcate-package DMRcate
> 
> ### ** Examples
> 
> library(ExperimentHub)
Loading required package: BiocGenerics
Loading required package: generics

Attaching package: 'generics'

The following objects are masked from 'package:base':

    as.difftime, as.factor, as.ordered, intersect, is.element, setdiff,
    setequal, union


Attaching package: 'BiocGenerics'

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, is.unsorted, lapply,
    mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int,
    rank, rbind, rownames, sapply, saveRDS, table, tapply, unique,
    unsplit, which.max, which.min

Loading required package: AnnotationHub
Loading required package: BiocFileCache
Loading required package: dbplyr
> library(limma)

Attaching package: 'limma'

The following object is masked from 'package:BiocGenerics':

    plotMA

> eh <- ExperimentHub()
> FlowSorted.Blood.EPIC <- eh[["EH1136"]]
see ?FlowSorted.Blood.EPIC and browseVignettes('FlowSorted.Blood.EPIC') for documentation
loading from cache
> tcell <- FlowSorted.Blood.EPIC[,colData(FlowSorted.Blood.EPIC)$CD4T==100 |
+                                 colData(FlowSorted.Blood.EPIC)$CD8T==100]
> detP <- minfi::detectionP(tcell)
Loading required package: IlluminaHumanMethylationEPICmanifest
> remove <- apply(detP, 1, function (x) any(x > 0.01))
> tcell <- tcell[!remove,]
> tcell <- minfi::preprocessFunnorm(tcell)
[preprocessFunnorm] Background and dye bias correction with noob
Loading required package: IlluminaHumanMethylationEPICanno.ilm10b4.hg19
[preprocessFunnorm] Mapping to genome
[preprocessFunnorm] Quantile extraction
[preprocessFunnorm] Normalization
> #Subset to chr2 only
> tcell <- tcell[seqnames(tcell) == "chr2",]
> tcellms <- minfi::getM(tcell)
> tcellms.noSNPs <- rmSNPandCH(tcellms, dist=2, mafcut=0.05)
Probe IDs from EPICv1 or earlier detected. Proceeding...
see ?DMRcatedata and browseVignettes('DMRcatedata') for documentation
loading from cache
see ?DMRcatedata and browseVignettes('DMRcatedata') for documentation
loading from cache
> tcell$Replicate[tcell$Replicate==""] <- tcell$Sample_Name[tcell$Replicate==""]
> tcellms.noSNPs <- avearrays(tcellms.noSNPs, tcell$Replicate)
> tcell <- tcell[,!duplicated(tcell$Replicate)]
> tcell <- tcell[rownames(tcellms.noSNPs),]
> colnames(tcellms.noSNPs) <- colnames(tcell)
> assays(tcell)[["M"]] <- tcellms.noSNPs
> assays(tcell)[["Beta"]] <- minfi::ilogit2(tcellms.noSNPs)
> type <- factor(tcell$CellType)
> design <- model.matrix(~type) 
> myannotation <- cpg.annotate("array", tcell, 
+                              arraytype = "EPICv1", analysis.type="differential",
+                              design=design, coef=2)
Your contrast returned 2694 individually significant probes. We recommend the default setting of pcutoff in dmrcate().
> dmrcoutput <- dmrcate(myannotation, lambda=1000, C=2)
Fitting chr2...
Demarcating regions...
Done!
> results.ranges <- extractRanges(dmrcoutput, genome = "hg19")
see ?DMRcatedata and browseVignettes('DMRcatedata') for documentation
loading from cache
> groups <- c(CD8T="magenta", CD4T="forestgreen")
> cols <- groups[as.character(type)]
> DMR.plot(ranges=results.ranges, dmr=1, 
+          CpGs=minfi::getBeta(tcell), what="Beta", 
+          arraytype = "EPICv1", phen.col=cols, genome="hg19")
Error in `req_perform()`:
! HTTP 504 Gateway Timeout.
Backtrace:
     ▆
  1. └─DMRcate::DMR.plot(...)
  2.   ├─base::suppressWarnings(...)
  3.   │ └─base::withCallingHandlers(...)
  4.   └─biomaRt::useEnsembl(...)
  5.     └─biomaRt::useDataset(mart = mart, dataset = dataset, verbose = verbose)
  6.       └─biomaRt:::.getAttributes(mart, verbose = verbose)
  7.         └─biomaRt:::.getAttrFilt(mart = mart, verbose = verbose, type = "attributes")
  8.           └─biomaRt:::bmRequest(...)
  9.             └─httr2::req_perform(request)
 10.               └─httr2:::handle_resp(req, resp, error_call = error_call)
 11.                 └─httr2:::resp_failure_cnd(req, resp, error_call = error_call)
 12.                   ├─rlang::catch_cnd(...)
 13.                   │ ├─rlang::eval_bare(...)
 14.                   │ ├─base::tryCatch(...)
 15.                   │ │ └─base (local) tryCatchList(expr, classes, parentenv, handlers)
 16.                   │ │   └─base (local) tryCatchOne(expr, names, parentenv, handlers[[1L]])
 17.                   │ │     └─base (local) doTryCatch(return(expr), name, parentenv, handler)
 18.                   │ └─base::force(expr)
 19.                   └─rlang::abort(...)
Execution halted
Examples with CPU (user + system) or elapsed time > 5s
           user system elapsed
DMR.plot 65.996  0.898  83.781
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE

Status: 1 ERROR
See
  ‘/Users/biocbuild/bbs-3.23-bioc/meat/DMRcate.Rcheck/00check.log’
for details.

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###
### Running command:
###
###   /Library/Frameworks/R.framework/Resources/bin/R CMD INSTALL DMRcate
###
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* installing to library ‘/Library/Frameworks/R.framework/Versions/4.6-arm64/Resources/library’
* installing *source* package ‘DMRcate’ ...
** this is package ‘DMRcate’ version ‘3.7.0’
** using staged installation
** R
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (DMRcate)