This page was generated on 2018-10-17 08:24:39 -0400 (Wed, 17 Oct 2018).
HTSeqGenie 4.10.0 Jens Reeder
Snapshot Date: 2018-10-15 16:45:08 -0400 (Mon, 15 Oct 2018) |
URL: https://git.bioconductor.org/packages/HTSeqGenie |
Branch: RELEASE_3_7 |
Last Commit: 39182b1 |
Last Changed Date: 2018-04-30 10:35:27 -0400 (Mon, 30 Apr 2018) |
| malbec2 | Linux (Ubuntu 16.04.1 LTS) / x86_64 | OK | OK | [ OK ] | | |
tokay2 | Windows Server 2012 R2 Standard / x64 | ...NOT SUPPORTED... |
merida2 | OS X 10.11.6 El Capitan / x86_64 | ...NOT SUPPORTED... |
R version 3.5.1 Patched (2018-07-12 r74967) -- "Feather Spray"
Copyright (C) 2018 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu (64-bit)
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> library("HTSeqGenie")
Loading required package: gmapR
Loading required package: GenomeInfoDb
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
Filter, Find, Map, Position, Reduce, anyDuplicated, append,
as.data.frame, basename, cbind, colMeans, colSums, colnames,
dirname, do.call, duplicated, eval, evalq, get, grep, grepl,
intersect, is.unsorted, lapply, lengths, mapply, match, mget,
order, paste, pmax, pmax.int, pmin, pmin.int, rank, rbind,
rowMeans, rowSums, rownames, sapply, setdiff, sort, table, tapply,
union, unique, unsplit, which, which.max, which.min
Loading required package: S4Vectors
Loading required package: stats4
Attaching package: 'S4Vectors'
The following object is masked from 'package:base':
expand.grid
Loading required package: IRanges
Loading required package: GenomicRanges
Loading required package: Rsamtools
Loading required package: Biostrings
Loading required package: XVector
Attaching package: 'Biostrings'
The following object is masked from 'package:base':
strsplit
Loading required package: ShortRead
Loading required package: BiocParallel
Loading required package: GenomicAlignments
Loading required package: SummarizedExperiment
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with
'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: DelayedArray
Loading required package: matrixStats
Attaching package: 'matrixStats'
The following objects are masked from 'package:Biobase':
anyMissing, rowMedians
Attaching package: 'DelayedArray'
The following objects are masked from 'package:matrixStats':
colMaxs, colMins, colRanges, rowMaxs, rowMins, rowRanges
The following object is masked from 'package:Biostrings':
type
The following objects are masked from 'package:base':
aperm, apply
Loading required package: VariantAnnotation
Attaching package: 'VariantAnnotation'
The following object is masked from 'package:base':
tabulate
>
> source(getPackageFile("unitTests/runTests.R"))
Loading required package: GenomicFeatures
Loading required package: AnnotationDbi
did not source anything in dirname= ./R
Executing test function test.alignReads ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:14:47 INFO::preprocessReads.R/preprocessReads: starting...
2018-10-16 01:14:47 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:14:47 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_2.fastq
2018-10-16 01:14:47 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:14:49 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:14:49 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/chunks/chunk_000001/logs/progress.log
2018-10-16 01:14:52 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.049 minutes
2018-10-16 01:14:52 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/chunks/chunk_000002/logs/progress.log
2018-10-16 01:14:54 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.045 minutes
2018-10-16 01:14:54 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/chunks/chunk_000003/logs/progress.log
2018-10-16 01:14:57 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.045 minutes
2018-10-16 01:14:57 DEBUG::tools.R/processChunks: done
2018-10-16 01:14:57 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ...
2018-10-16 01:14:57 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/results/test_pe.adapter_contaminated_1.RData
2018-10-16 01:14:57 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ...
2018-10-16 01:14:57 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/results/test_pe.adapter_contaminated_2.RData
2018-10-16 01:14:57 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=26 highqual_reads=25 adapter_contam=1 read_length=75 rRNA_contam_reads=0 processed_reads=25 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75
2018-10-16 01:14:57 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/results/test_pe.summary_preprocess.tab
2018-10-16 01:14:57 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/bams/processed.aligner_input_1.fastq ...
2018-10-16 01:14:57 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/bams/processed.aligner_input_2.fastq ...
2018-10-16 01:14:57 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/reports/shortReadReport_1 ...
2018-10-16 01:14:59 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/reports/shortReadReport_2 ...
2018-10-16 01:15:00 INFO::preprocessReads.R/preprocessReads: done
2018-10-16 01:15:00 INFO::alignReads.R/alignReads: starting alignment...
2018-10-16 01:15:00 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:15:02 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:15:02 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/chunks/chunk_000001/logs/progress.log
2018-10-16 01:15:08 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.093 minutes
2018-10-16 01:15:08 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/chunks/chunk_000002/logs/progress.log
2018-10-16 01:15:10 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.043 minutes
2018-10-16 01:15:10 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/chunks/chunk_000003/logs/progress.log
2018-10-16 01:15:13 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.043 minutes
2018-10-16 01:15:13 DEBUG::tools.R/processChunks: done
2018-10-16 01:15:13 INFO::alignReads.R/mergeBAMsAcrossDirs: starting...
2018-10-16 01:15:13 INFO::alignReads.R/mergeBAMsAcrossDirs: done
2018-10-16 01:15:13 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/results/test_pe.summary_alignment.tab
2018-10-16 01:15:13 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/results/test_pe.summary_analyzed_bamstats.tab
2018-10-16 01:15:13 INFO::reportPipelineQA.R/writePreprocessAlignReport: creating joint preprocess-alignment report...
2018-10-16 01:15:13 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.alignReads.8cb6cb33f38/results/test_pe.summary_target_lengths.tab
2018-10-16 01:15:13 INFO::reportPipelineQA.R/writePreprocessAlignReport: done
2018-10-16 01:15:13 INFO::alignReads.R/alignReads: done
done successfully.
Executing test function test.alignReads.sparsechunks ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:15:14 INFO::preprocessReads.R/preprocessReads: starting...
2018-10-16 01:15:14 INFO::io.R/FastQStreamer.init: counting number of reads in file= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:15:14 INFO::io.R/FastQStreamer.init: subsampling_filter set (subsampled reads= 2 , totnbreads= 26 )
2018-10-16 01:15:14 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:15:14 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_2.fastq
2018-10-16 01:15:14 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:15:16 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:15:16 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp9OnOUL/test.alignReads.sparsechunks.8cb5ea10cc7/chunks/chunk_000001/logs/progress.log
2018-10-16 01:15:19 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.045 minutes
2018-10-16 01:15:19 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp9OnOUL/test.alignReads.sparsechunks.8cb5ea10cc7/chunks/chunk_000002/logs/progress.log
2018-10-16 01:15:21 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.047 minutes
2018-10-16 01:15:21 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp9OnOUL/test.alignReads.sparsechunks.8cb5ea10cc7/chunks/chunk_000003/logs/progress.log
2018-10-16 01:15:24 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.044 minutes
2018-10-16 01:15:24 DEBUG::tools.R/processChunks: starting chunkid= 4 ; see logfile= /tmp/Rtmp9OnOUL/test.alignReads.sparsechunks.8cb5ea10cc7/chunks/chunk_000004/logs/progress.log
2018-10-16 01:15:27 DEBUG::tools.R/processChunks: done with chunkid= 4 ; elapsed.time= 0.044 minutes
2018-10-16 01:15:27 DEBUG::tools.R/processChunks: done
2018-10-16 01:15:27 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ...
2018-10-16 01:15:27 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.alignReads.sparsechunks.8cb5ea10cc7/results/test_pe.adapter_contaminated_1.RData
2018-10-16 01:15:27 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ...
2018-10-16 01:15:27 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.alignReads.sparsechunks.8cb5ea10cc7/results/test_pe.adapter_contaminated_2.RData
2018-10-16 01:15:27 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=2 highqual_reads=1 adapter_contam=0 read_length=75 rRNA_contam_reads=0 processed_reads=1 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75
2018-10-16 01:15:27 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.alignReads.sparsechunks.8cb5ea10cc7/results/test_pe.summary_preprocess.tab
2018-10-16 01:15:27 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp9OnOUL/test.alignReads.sparsechunks.8cb5ea10cc7/bams/processed.aligner_input_1.fastq ...
2018-10-16 01:15:27 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp9OnOUL/test.alignReads.sparsechunks.8cb5ea10cc7/bams/processed.aligner_input_2.fastq ...
2018-10-16 01:15:27 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp9OnOUL/test.alignReads.sparsechunks.8cb5ea10cc7/reports/shortReadReport_1 ...
2018-10-16 01:15:28 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp9OnOUL/test.alignReads.sparsechunks.8cb5ea10cc7/reports/shortReadReport_2 ...
2018-10-16 01:15:30 INFO::preprocessReads.R/preprocessReads: done
done successfully.
Executing test function test.alignReadsOneSingleEnd ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:15:30 INFO::alignReads.R/alignReadsChunk: running gsnap...
2018-10-16 01:15:30 INFO::alignReads.R/alignReadsChunk: calling gsnap (from gmapR) with args= -D /home/biocbuild/.local/share/gmap -t 1 -d TP53_demo_3.2.2 --quality-protocol=illumina -A sam --read-group-id=test_se -m 0 --split-output /tmp/Rtmp9OnOUL/test.alignReadsOneSingleEnd.8cb412a2579/bams/test.alignReads /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq 2>&1
2018-10-16 01:15:30 INFO::alignReads.R/createSummaryAlignment: counting unique bam reads...
2018-10-16 01:15:30 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.alignReadsOneSingleEnd.8cb412a2579/results/test.alignReads.summary_alignment.tab
2018-10-16 01:15:30 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.alignReadsOneSingleEnd.8cb412a2579/results/test.alignReads.summary_analyzed_bamstats.tab
2018-10-16 01:15:30 INFO::alignReads.R/alignReadsChunk: done
done successfully.
Executing test function test.annotateVariants ... Timing stopped at: 0 0.052 0.057
Error in DEACTIVATED("Skipped annotateVariants() test") :
Skipped annotateVariants() test
In addition: There were 19 warnings (use warnings() to see them)
done successfully.
Executing test function test.callVariantsVariantTools.genotype ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:15:31 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.genotype.8cb4a490485/results/test_pe.coverage.RData
2018-10-16 01:15:31 INFO::coverage.R/saveCoverage: saving file= /tmp/Rtmp9OnOUL/test.genotype.8cb4a490485/results/test_pe.coverage.bw
2018-10-16 01:15:31 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.genotype.8cb4a490485/results/test_pe.summary_coverage.tab
2018-10-16 01:15:31 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants...
2018-10-16 01:15:34 INFO::analyzeVariants.R/wrap.callVariants: calling variants...
2018-10-16 01:15:34 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants...
2018-10-16 01:15:34 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants...
2018-10-16 01:15:34 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.genotype.8cb4a490485/results/test_pe.raw_variants.RData
2018-10-16 01:15:34 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.genotype.8cb4a490485/results/test_pe.filtered_variants.RData
2018-10-16 01:15:34 INFO::analyzeVariants.R/wrap.callVariants: ...done
2018-10-16 01:15:34 INFO::analyzeVariants.R/writeVCF: writing vcf file...
2018-10-16 01:15:34 INFO::analyzeVariants.R/writeVCF: ...done
2018-10-16 01:15:34 INFO::analyzeVariants.R/.callGenotypes: calling genotypes...
2018-10-16 01:16:31 INFO::analyzeVariants.R/writeVCF: writing vcf file...
2018-10-16 01:16:32 INFO::analyzeVariants.R/writeVCF: ...done
2018-10-16 01:16:32 INFO::analyzeVariants.R/.callGenotypes: done
done successfully.
Executing test function test.wrap.callVariants ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:16:34 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants...
2018-10-16 01:16:37 INFO::analyzeVariants.R/wrap.callVariants: calling variants...
2018-10-16 01:16:37 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants...
2018-10-16 01:16:38 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants...
2018-10-16 01:16:38 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.wrap.callVariants.8cb15983755/results/test_pe.raw_variants.RData
2018-10-16 01:16:38 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.wrap.callVariants.8cb15983755/results/test_pe.filtered_variants.RData
2018-10-16 01:16:38 INFO::analyzeVariants.R/wrap.callVariants: ...done
done successfully.
Executing test function test.wrap.callVariants.filters ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:16:38 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants...
2018-10-16 01:16:41 INFO::analyzeVariants.R/wrap.callVariants: calling variants...
2018-10-16 01:16:41 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants...
2018-10-16 01:16:41 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants...
2018-10-16 01:16:41 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.wrap.callVariants.filters.8cb284a6988/results/test_pe.raw_variants.RData
2018-10-16 01:16:41 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.wrap.callVariants.filters.8cb284a6988/results/test_pe.filtered_variants.RData
2018-10-16 01:16:41 INFO::analyzeVariants.R/wrap.callVariants: ...done
2018-10-16 01:16:41 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants...
2018-10-16 01:16:45 INFO::analyzeVariants.R/wrap.callVariants: calling variants...
2018-10-16 01:16:45 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants...
2018-10-16 01:16:45 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants...
2018-10-16 01:16:45 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.wrap.callVariants.filters.8cb284a6988/results/test_pe.raw_variants.RData
2018-10-16 01:16:45 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.wrap.callVariants.filters.8cb284a6988/results/test_pe.filtered_variants.RData
2018-10-16 01:16:45 INFO::analyzeVariants.R/wrap.callVariants: ...done
done successfully.
Executing test function test.wrap.callVariants.parallel ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:16:46 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants...
2018-10-16 01:16:50 INFO::analyzeVariants.R/wrap.callVariants: calling variants...
2018-10-16 01:16:50 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants...
2018-10-16 01:16:50 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants...
2018-10-16 01:16:50 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.wrap.callVariants.parallel.8cbbec7b78/results/test_pe.raw_variants.RData
2018-10-16 01:16:50 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.wrap.callVariants.parallel.8cbbec7b78/results/test_pe.filtered_variants.RData
2018-10-16 01:16:50 INFO::analyzeVariants.R/wrap.callVariants: ...done
done successfully.
Executing test function test.wrap.callVariants.which ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:16:51 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants...
2018-10-16 01:16:51 INFO::analyzeVariants.R/buildTallyParam: restricting variant calls using 'which'
2018-10-16 01:16:52 INFO::analyzeVariants.R/wrap.callVariants: calling variants...
2018-10-16 01:16:52 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants...
2018-10-16 01:16:53 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants...
2018-10-16 01:16:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.wrap.callVariants.which.8cb7eaba93d/results/test_pe.raw_variants.RData
2018-10-16 01:16:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.wrap.callVariants.which.8cb7eaba93d/results/test_pe.filtered_variants.RData
2018-10-16 01:16:53 INFO::analyzeVariants.R/wrap.callVariants: ...done
done successfully.
Executing test function test.writeVCF.NULL ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:16:53 INFO::analyzeVariants.R/writeVCF: writing vcf file...
2018-10-16 01:16:53 INFO::analyzeVariants.R/writeVCF: ...done
done successfully.
Executing test function test.writeVCF.vcfStat ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:16:54 INFO::analyzeVariants.R/writeVCF: writing vcf file...
2018-10-16 01:16:54 INFO::analyzeVariants.R/writeVCF: ...done
done successfully.
Executing test function test.isFirstFragment ... done successfully.
Executing test function test.buildCountsGRangesList ... done successfully.
Executing test function test.generateSingleGeneDERs ... done successfully.
Executing test function test.computeCoverage ... done successfully.
Executing test function test.isSparse ... done successfully.
Executing test function test.mergeCoverage ... 2018-10-16 01:17:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/oiqdcwtk/merged/results/bla.coverage.RData
2018-10-16 01:17:53 INFO::coverage.R/saveCoverage: saving file= /tmp/Rtmp9OnOUL/oiqdcwtk/merged/results/bla.coverage.bw
2018-10-16 01:17:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/oiqdcwtk/merged/results/bla.summary_coverage.tab
done successfully.
Executing test function test.mergeCoverage.sparse ... 2018-10-16 01:17:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/rvjuxstz/merged/results/bla.coverage.RData
2018-10-16 01:17:54 INFO::coverage.R/saveCoverage: saving file= /tmp/Rtmp9OnOUL/rvjuxstz/merged/results/bla.coverage.bw
2018-10-16 01:17:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/rvjuxstz/merged/results/bla.summary_coverage.tab
done successfully.
Executing test function test.checkConfig.analyzeVariants ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
done successfully.
Executing test function test.findTemplate ... done successfully.
Executing test function test.checkConfig ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
done successfully.
Executing test function test.checkConfig.alignReads ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
done successfully.
Executing test function test.getConfig ... done successfully.
Executing test function test.loadConfig ... done successfully.
Executing test function test.parseDCF ... done successfully.
Executing test function test.updateConfig ... done successfully.
Executing test function test.getAdapterSeqs ... done successfully.
Executing test function test.isAdapter ... done successfully.
Executing test function test.isAdapter3.primeEnd ... done successfully.
Executing test function test.detectRRNA ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:17:58 INFO::detectRRNA.R/detectRRNA: searching for rRNA contamination...
2018-10-16 01:17:58 INFO::io.R/writeFastQFiles: writing filename= /tmp/Rtmp9OnOUL/test.detectRRNA.8cb71ef694a/bams/rRNA_contam/input1.fastq
2018-10-16 01:17:58 INFO::alignReads.R/alignReadsChunk: calling gsnap (from gmapR) with args= -t 0 -D /home/biocbuild/.local/share/gmap -n 1 -d TP53_demo_3.2.2 -A sam -B 2 --pairmax-rna=200000 --split-output /tmp/Rtmp9OnOUL/test.detectRRNA.8cb71ef694a/bams/rRNA_contam/test_se /tmp/Rtmp9OnOUL/test.detectRRNA.8cb71ef694a/bams/rRNA_contam/input1.fastq 2>&1
2018-10-16 01:17:59 INFO::detectRRNA.R/detectRRNA: contaminated fraction= 1
2018-10-16 01:17:59 INFO::detectRRNA.R/detectRRNA: done
done successfully.
Executing test function test.detectRRNA.paired_end ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:17:59 INFO::detectRRNA.R/detectRRNA: searching for rRNA contamination...
2018-10-16 01:17:59 INFO::io.R/writeFastQFiles: writing filename= /tmp/Rtmp9OnOUL/test.detectRRNA.paired_end.8cb15ed290e/bams/rRNA_contam/input1.fastq
2018-10-16 01:17:59 INFO::io.R/writeFastQFiles: writing filename= /tmp/Rtmp9OnOUL/test.detectRRNA.paired_end.8cb15ed290e/bams/rRNA_contam/input2.fastq
2018-10-16 01:17:59 INFO::alignReads.R/alignReadsChunk: calling gsnap (from gmapR) with args= -t 0 -D /home/biocbuild/.local/share/gmap -n 1 -d TP53_demo_3.2.2 -A sam -B 2 --pairmax-rna=200000 --split-output /tmp/Rtmp9OnOUL/test.detectRRNA.paired_end.8cb15ed290e/bams/rRNA_contam/test_pe /tmp/Rtmp9OnOUL/test.detectRRNA.paired_end.8cb15ed290e/bams/rRNA_contam/input1.fastq -a paired /tmp/Rtmp9OnOUL/test.detectRRNA.paired_end.8cb15ed290e/bams/rRNA_contam/input2.fastq 2>&1
2018-10-16 01:18:00 INFO::detectRRNA.R/detectRRNA: contaminated fraction= 1
2018-10-16 01:18:00 INFO::detectRRNA.R/detectRRNA: done
done successfully.
Executing test function test.getRRNAIds ... 2018-10-16 01:18:00 INFO::alignReads.R/alignReadsChunk: calling gsnap (from gmapR) with args= -t 0 -D /home/biocbuild/.local/share/gmap -n 1 -d TP53_demo_3.2.2 -A sam -B 2 --pairmax-rna=200000 --split-output /tmp/Rtmp9OnOUL/test_get_rRNA_idsomshtvze/test_pe /tmp/Rtmp9OnOUL/test_get_rRNA_idsomshtvze/1.fastq -a paired /tmp/Rtmp9OnOUL/test_get_rRNA_idsomshtvze/2.fastq 2>&1
done successfully.
Executing test function test.getRRNAIds_random ... 2018-10-16 01:18:00 INFO::alignReads.R/alignReadsChunk: calling gsnap (from gmapR) with args= -t 0 -D /home/biocbuild/.local/share/gmap -n 1 -d TP53_demo_3.2.2 -A sam -B 2 --pairmax-rna=200000 --split-output /tmp/Rtmp9OnOUL/test_get_rRNAIds_randomlsbuvika/test_pe /tmp/Rtmp9OnOUL/test_get_rRNAIds_randomlsbuvika/1.fastq 2>&1
done successfully.
Executing test function test.filterByLength ... 2018-10-16 01:18:00 INFO::filterQuality.R/filterQuality: filterByLength...
2018-10-16 01:18:00 INFO::filterQuality.R/filterByLength: fraction of filtered short reads= 0.5
2018-10-16 01:18:00 INFO::filterQuality.R/filterByLength: done
2018-10-16 01:18:00 INFO::filterQuality.R/filterQuality: filterByLength...
2018-10-16 01:18:00 INFO::filterQuality.R/filterByLength: fraction of filtered short reads= 1
2018-10-16 01:18:00 INFO::filterQuality.R/filterByLength: done
done successfully.
Executing test function test.isAboveQualityThresh ... done successfully.
Executing test function test.trimTailsByQuality ... 2018-10-16 01:18:01 INFO::preprocessReads.R/preprocessReadsChunk: Starting trimTailsByQuality ...
2018-10-16 01:18:01 INFO::preprocessReads.R/preprocessReadsChunk: done
2018-10-16 01:18:01 INFO::preprocessReads.R/preprocessReadsChunk: Starting trimTailsByQuality ...
2018-10-16 01:18:01 INFO::preprocessReads.R/preprocessReadsChunk: done
2018-10-16 01:18:01 INFO::preprocessReads.R/preprocessReadsChunk: Starting trimTailsByQuality ...
2018-10-16 01:18:01 INFO::preprocessReads.R/preprocessReadsChunk: done
2018-10-16 01:18:01 INFO::preprocessReads.R/preprocessReadsChunk: Starting trimTailsByQuality ...
2018-10-16 01:18:01 INFO::preprocessReads.R/preprocessReadsChunk: done
done successfully.
Executing test function test.callVariantsGATK ... Timing stopped at: 0.004 0 0
Error in DEACTIVATED("callVariantsGATK() tests need gatk.path option set") :
callVariantsGATK() tests need gatk.path option set
In addition: There were 27 warnings (use warnings() to see them)
done successfully.
Executing test function test.callVariantsGATK.withFiltering ... Timing stopped at: 0 0 0
Error in DEACTIVATED("callVariantsGATK() tests need gatk.path option set") :
callVariantsGATK() tests need gatk.path option set
done successfully.
Executing test function test.checkGATKJar ... Timing stopped at: 0 0 0
Error in DEACTIVATED("checkGATKJar() test needs gatk.path option set") :
checkGATKJar() test needs gatk.path option set
done successfully.
Executing test function test.excludeVariantsByRegion ... done successfully.
Executing test function test.gatk ... Timing stopped at: 0 0 0.001
Error in DEACTIVATED("gatk() tests need gatk.path option set") :
gatk() tests need gatk.path option set
done successfully.
Executing test function test.realignIndels ... Timing stopped at: 0 0 0
Error in DEACTIVATED("test.realignIndels() tests need gatk.path option set") :
test.realignIndels() tests need gatk.path option set
done successfully.
Executing test function test.realignIndelsGATK ... Timing stopped at: 0 0 0
Error in DEACTIVATED("realignIndelsGATK() tests need gatk.path option set") :
realignIndelsGATK() tests need gatk.path option set
done successfully.
Executing test function test.realignIndelsGATK.parallel ... Timing stopped at: 0 0 0
Error in DEACTIVATED("realignIndelsGATK() tests need gatk.path option set") :
realignIndelsGATK() tests need gatk.path option set
done successfully.
Executing test function test_zipUp ... done successfully.
Executing test function test.FastQStreamer.getReads.pefq ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:18:02 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:18:02 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_2.fastq
done successfully.
Executing test function test.FastQStreamer.getReads.pefq.subsample ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:18:02 INFO::io.R/FastQStreamer.init: counting number of reads in file= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:18:02 INFO::io.R/FastQStreamer.init: subsampling_filter set (subsampled reads= 17 , totnbreads= 26 )
2018-10-16 01:18:02 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:18:02 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_2.fastq
done successfully.
Executing test function test.FastQStreamer.getReads.segz ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:18:03 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/reads.fastq.gz
done successfully.
Executing test function test.FastQStreamer.getReads.truncated ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:18:04 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/truncated.fastq.gz
done successfully.
Executing test function test.FastQStreamer.subsampler.isdeterministic ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:18:05 INFO::io.R/FastQStreamer.init: counting number of reads in file= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:18:05 INFO::io.R/FastQStreamer.init: subsampling_filter set (subsampled reads= 17 , totnbreads= 26 )
2018-10-16 01:18:05 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:18:05 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_2.fastq
checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:18:05 INFO::io.R/FastQStreamer.init: counting number of reads in file= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:18:06 INFO::io.R/FastQStreamer.init: subsampling_filter set (subsampled reads= 17 , totnbreads= 26 )
2018-10-16 01:18:06 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:18:06 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_2.fastq
done successfully.
Executing test function test.createTmpDir ... done successfully.
Executing test function test.detectQualityInFASTQFile ... done successfully.
Executing test function test.getObjectFilename ... done successfully.
Executing test function test.safeUnlink ... done successfully.
Executing test function test.writeAudit ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
done successfully.
Executing test function test.mergeLanes ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:18:09 INFO::preprocessReads.R/preprocessReads: starting...
2018-10-16 01:18:09 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:18:09 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_2.fastq
2018-10-16 01:18:09 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:18:11 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:18:11 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/chunks/chunk_000001/logs/progress.log
2018-10-16 01:18:13 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.043 minutes
2018-10-16 01:18:13 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/chunks/chunk_000002/logs/progress.log
2018-10-16 01:18:16 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.044 minutes
2018-10-16 01:18:16 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/chunks/chunk_000003/logs/progress.log
2018-10-16 01:18:19 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.045 minutes
2018-10-16 01:18:19 DEBUG::tools.R/processChunks: done
2018-10-16 01:18:19 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ...
2018-10-16 01:18:19 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.adapter_contaminated_1.RData
2018-10-16 01:18:19 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ...
2018-10-16 01:18:19 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.adapter_contaminated_2.RData
2018-10-16 01:18:19 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=26 highqual_reads=25 adapter_contam=1 read_length=75 rRNA_contam_reads=0 processed_reads=25 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75
2018-10-16 01:18:19 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.summary_preprocess.tab
2018-10-16 01:18:19 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/bams/processed.aligner_input_1.fastq ...
2018-10-16 01:18:19 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/bams/processed.aligner_input_2.fastq ...
2018-10-16 01:18:19 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/reports/shortReadReport_1 ...
2018-10-16 01:18:20 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/reports/shortReadReport_2 ...
2018-10-16 01:18:22 INFO::preprocessReads.R/preprocessReads: done
2018-10-16 01:18:22 INFO::alignReads.R/alignReads: starting alignment...
2018-10-16 01:18:22 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:18:24 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:18:24 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/chunks/chunk_000001/logs/progress.log
2018-10-16 01:18:26 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.043 minutes
2018-10-16 01:18:26 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/chunks/chunk_000002/logs/progress.log
2018-10-16 01:18:29 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.043 minutes
2018-10-16 01:18:29 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/chunks/chunk_000003/logs/progress.log
2018-10-16 01:18:31 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.044 minutes
2018-10-16 01:18:31 DEBUG::tools.R/processChunks: done
2018-10-16 01:18:31 INFO::alignReads.R/mergeBAMsAcrossDirs: starting...
2018-10-16 01:18:32 INFO::alignReads.R/mergeBAMsAcrossDirs: done
2018-10-16 01:18:32 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.summary_alignment.tab
2018-10-16 01:18:32 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.summary_analyzed_bamstats.tab
2018-10-16 01:18:32 INFO::reportPipelineQA.R/writePreprocessAlignReport: creating joint preprocess-alignment report...
2018-10-16 01:18:32 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.summary_target_lengths.tab
2018-10-16 01:18:32 INFO::reportPipelineQA.R/writePreprocessAlignReport: done
2018-10-16 01:18:32 INFO::alignReads.R/alignReads: done
2018-10-16 01:18:32 INFO::countGenomicFeatures.R/countGenomicFeatures: starting...
2018-10-16 01:18:32 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:18:34 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:18:34 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/chunks/chunk_000001/logs/progress.log
2018-10-16 01:18:36 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.039 minutes
2018-10-16 01:18:36 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/chunks/chunk_000002/logs/progress.log
2018-10-16 01:18:39 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.04 minutes
2018-10-16 01:18:39 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/chunks/chunk_000003/logs/progress.log
2018-10-16 01:18:41 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.04 minutes
2018-10-16 01:18:41 DEBUG::tools.R/processChunks: done
2018-10-16 01:18:41 INFO::countGenomicFeatures.R/mergeCounts: starting...
2018-10-16 01:18:41 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.counts_exon.tab
2018-10-16 01:18:41 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.counts_exon_disjoint.tab
2018-10-16 01:18:41 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.counts_gene.tab
2018-10-16 01:18:41 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.counts_gene_coding.tab
2018-10-16 01:18:41 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.counts_gene_exonic.tab
2018-10-16 01:18:41 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.counts_intergenic.tab
2018-10-16 01:18:41 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.counts_intron.tab
2018-10-16 01:18:41 INFO::countGenomicFeatures.R/mergeCounts: done
2018-10-16 01:18:41 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.summary_counts.tab
2018-10-16 01:18:41 INFO::reportPipelineQA.R/writeGenomicFeaturesReport: creating report of genomic features counts...
2018-10-16 01:18:42 INFO::reportPipelineQA.R/writeGenomicFeaturesReport: done
2018-10-16 01:18:42 INFO::countGenomicFeatures.R/countGenomicFeatures: done...
2018-10-16 01:18:42 INFO::coverage.R/calculateCoverage: starting...
2018-10-16 01:18:42 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:18:44 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:18:44 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/chunks/chunk_000001/logs/progress.log
2018-10-16 01:18:46 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.036 minutes
2018-10-16 01:18:46 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/chunks/chunk_000002/logs/progress.log
2018-10-16 01:18:48 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.036 minutes
2018-10-16 01:18:48 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/chunks/chunk_000003/logs/progress.log
2018-10-16 01:18:50 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.036 minutes
2018-10-16 01:18:50 DEBUG::tools.R/processChunks: done
2018-10-16 01:18:51 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.coverage.RData
2018-10-16 01:18:51 INFO::coverage.R/saveCoverage: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.coverage.bw
2018-10-16 01:18:51 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.summary_coverage.tab
2018-10-16 01:18:51 INFO::coverage.R/calculateCoverage: done
2018-10-16 01:18:51 INFO::analyzeVariants/analyzeVariants: starting ...
2018-10-16 01:18:51 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants...
2018-10-16 01:18:52 INFO::analyzeVariants.R/wrap.callVariants: calling variants...
2018-10-16 01:18:52 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants...
2018-10-16 01:18:52 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants...
2018-10-16 01:18:52 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.raw_variants.RData
2018-10-16 01:18:52 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.filtered_variants.RData
2018-10-16 01:18:52 INFO::analyzeVariants.R/wrap.callVariants: ...done
2018-10-16 01:18:52 INFO::analyzeVariants.R/writeVCF: writing vcf file...
2018-10-16 01:18:53 INFO::analyzeVariants.R/writeVCF: ...done
2018-10-16 01:18:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/results/test_pe.summary_variants.tab
2018-10-16 01:18:53 INFO::analyzeVariants/analyzeVariants: done
2018-10-16 01:18:53 INFO::Pipeline run successful.
2018-10-16 01:18:53 INFO::mergeLanes.R/doMergeLanes: starting...
2018-10-16 01:18:53 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ...
2018-10-16 01:18:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.adapter_contaminated_1.RData
2018-10-16 01:18:53 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ...
2018-10-16 01:18:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.adapter_contaminated_2.RData
2018-10-16 01:18:53 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=26 highqual_reads=25 adapter_contam=1 read_length=75 rRNA_contam_reads=0 processed_reads=25 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75
2018-10-16 01:18:53 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.summary_preprocess.tab
2018-10-16 01:18:53 INFO::alignReads.R/mergeBAMsAcrossDirs: starting...
2018-10-16 01:18:53 INFO::alignReads.R/mergeBAMsAcrossDirs: done
2018-10-16 01:18:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.summary_alignment.tab
2018-10-16 01:18:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.summary_analyzed_bamstats.tab
2018-10-16 01:18:54 INFO::reportPipelineQA.R/writePreprocessAlignReport: creating joint preprocess-alignment report...
2018-10-16 01:18:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.summary_target_lengths.tab
2018-10-16 01:18:54 INFO::reportPipelineQA.R/writePreprocessAlignReport: done
2018-10-16 01:18:54 INFO::countGenomicFeatures.R/mergeCounts: starting...
2018-10-16 01:18:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.counts_exon.tab
2018-10-16 01:18:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.counts_exon_disjoint.tab
2018-10-16 01:18:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.counts_gene.tab
2018-10-16 01:18:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.counts_gene_coding.tab
2018-10-16 01:18:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.counts_gene_exonic.tab
2018-10-16 01:18:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.counts_intergenic.tab
2018-10-16 01:18:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.counts_intron.tab
2018-10-16 01:18:54 INFO::countGenomicFeatures.R/mergeCounts: done
2018-10-16 01:18:54 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.summary_counts.tab
2018-10-16 01:18:54 INFO::reportPipelineQA.R/writeGenomicFeaturesReport: creating report of genomic features counts...
2018-10-16 01:18:54 INFO::reportPipelineQA.R/writeGenomicFeaturesReport: done
2018-10-16 01:18:56 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.coverage.RData
2018-10-16 01:18:56 INFO::coverage.R/saveCoverage: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.coverage.bw
2018-10-16 01:18:56 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.summary_coverage.tab
2018-10-16 01:18:56 INFO::analyzeVariants/analyzeVariants: starting ...
2018-10-16 01:18:56 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants...
2018-10-16 01:18:57 INFO::analyzeVariants.R/wrap.callVariants: calling variants...
2018-10-16 01:18:57 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants...
2018-10-16 01:18:57 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants...
2018-10-16 01:18:57 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.raw_variants.RData
2018-10-16 01:18:57 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.filtered_variants.RData
2018-10-16 01:18:57 INFO::analyzeVariants.R/wrap.callVariants: ...done
2018-10-16 01:18:57 INFO::analyzeVariants.R/writeVCF: writing vcf file...
2018-10-16 01:18:57 INFO::analyzeVariants.R/writeVCF: ...done
2018-10-16 01:18:57 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.mergeLanes.8cb431957ef/merged/results/merged.summary_variants.tab
2018-10-16 01:18:57 INFO::analyzeVariants/analyzeVariants: done
2018-10-16 01:18:57 INFO::mergeLanes.R/doMergeLanes: merge lanes successful.
done successfully.
Executing test function test.markDuplicates ... Timing stopped at: 0 0 0.001
Error in DEACTIVATED("Skipped markDuplicates() test") :
Skipped markDuplicates() test
In addition: There were 50 or more warnings (use warnings() to see the first 50)
done successfully.
Executing test function test.markDuplicates_w_outfile ... Timing stopped at: 0 0 0.001
Error in DEACTIVATED("Skipped markDuplicates() test") :
Skipped markDuplicates() test
done successfully.
Executing test function test.preprocessReads ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:18:58 INFO::preprocessReads.R/preprocessReads: starting...
2018-10-16 01:18:58 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:18:58 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_2.fastq
2018-10-16 01:18:58 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:19:00 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:19:00 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp9OnOUL/test.preprocessReads.8cb79040786/chunks/chunk_000001/logs/progress.log
2018-10-16 01:19:03 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.043 minutes
2018-10-16 01:19:03 DEBUG::tools.R/processChunks: done
2018-10-16 01:19:03 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ...
2018-10-16 01:19:03 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.preprocessReads.8cb79040786/results/test_pe.adapter_contaminated_1.RData
2018-10-16 01:19:03 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ...
2018-10-16 01:19:03 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.preprocessReads.8cb79040786/results/test_pe.adapter_contaminated_2.RData
2018-10-16 01:19:03 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=26 highqual_reads=25 adapter_contam=1 read_length=75 rRNA_contam_reads=0 processed_reads=25 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75
2018-10-16 01:19:03 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.preprocessReads.8cb79040786/results/test_pe.summary_preprocess.tab
2018-10-16 01:19:03 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp9OnOUL/test.preprocessReads.8cb79040786/bams/processed.aligner_input_1.fastq ...
2018-10-16 01:19:03 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp9OnOUL/test.preprocessReads.8cb79040786/bams/processed.aligner_input_2.fastq ...
2018-10-16 01:19:03 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp9OnOUL/test.preprocessReads.8cb79040786/reports/shortReadReport_1 ...
2018-10-16 01:19:04 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp9OnOUL/test.preprocessReads.8cb79040786/reports/shortReadReport_2 ...
2018-10-16 01:19:06 INFO::preprocessReads.R/preprocessReads: done
done successfully.
Executing test function test.preprocessReads.minichunks ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:19:06 INFO::preprocessReads.R/preprocessReads: starting...
2018-10-16 01:19:06 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:19:06 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_2.fastq
2018-10-16 01:19:06 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:19:08 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:19:08 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp9OnOUL/test.preprocessReads.minichunks.8cb6374f060/chunks/chunk_000001/logs/progress.log
2018-10-16 01:19:11 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.044 minutes
2018-10-16 01:19:11 DEBUG::tools.R/processChunks: starting chunkid= 2 ; see logfile= /tmp/Rtmp9OnOUL/test.preprocessReads.minichunks.8cb6374f060/chunks/chunk_000002/logs/progress.log
2018-10-16 01:19:14 DEBUG::tools.R/processChunks: done with chunkid= 2 ; elapsed.time= 0.048 minutes
2018-10-16 01:19:14 DEBUG::tools.R/processChunks: starting chunkid= 3 ; see logfile= /tmp/Rtmp9OnOUL/test.preprocessReads.minichunks.8cb6374f060/chunks/chunk_000003/logs/progress.log
2018-10-16 01:19:16 DEBUG::tools.R/processChunks: done with chunkid= 3 ; elapsed.time= 0.044 minutes
2018-10-16 01:19:17 DEBUG::tools.R/processChunks: done
2018-10-16 01:19:17 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ...
2018-10-16 01:19:17 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.preprocessReads.minichunks.8cb6374f060/results/test_pe.adapter_contaminated_1.RData
2018-10-16 01:19:17 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ...
2018-10-16 01:19:17 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.preprocessReads.minichunks.8cb6374f060/results/test_pe.adapter_contaminated_2.RData
2018-10-16 01:19:17 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=26 highqual_reads=25 adapter_contam=1 read_length=75 rRNA_contam_reads=0 processed_reads=25 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75
2018-10-16 01:19:17 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.preprocessReads.minichunks.8cb6374f060/results/test_pe.summary_preprocess.tab
2018-10-16 01:19:17 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp9OnOUL/test.preprocessReads.minichunks.8cb6374f060/bams/processed.aligner_input_1.fastq ...
2018-10-16 01:19:17 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp9OnOUL/test.preprocessReads.minichunks.8cb6374f060/bams/processed.aligner_input_2.fastq ...
2018-10-16 01:19:17 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp9OnOUL/test.preprocessReads.minichunks.8cb6374f060/reports/shortReadReport_1 ...
2018-10-16 01:19:18 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp9OnOUL/test.preprocessReads.minichunks.8cb6374f060/reports/shortReadReport_2 ...
2018-10-16 01:19:20 INFO::preprocessReads.R/preprocessReads: done
done successfully.
Executing test function test.preprocessReads_single_end ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:19:20 INFO::preprocessReads.R/preprocessReads: starting...
2018-10-16 01:19:20 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/test-data/unit_tests_1.fastq
2018-10-16 01:19:20 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:19:22 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:19:22 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= /tmp/Rtmp9OnOUL/test.preprocessReads_single_end.8cb5bb8a8c4/chunks/chunk_000001/logs/progress.log
2018-10-16 01:19:25 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.04 minutes
2018-10-16 01:19:25 DEBUG::tools.R/processChunks: done
2018-10-16 01:19:25 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ...
2018-10-16 01:19:25 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.preprocessReads_single_end.8cb5bb8a8c4/results/test_se.adapter_contaminated_1.RData
2018-10-16 01:19:25 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=26 highqual_reads=25 adapter_contam=1 read_length=75 rRNA_contam_reads=0 processed_reads=25 input_min_read_length=75 input_max_read_length=75 processed_min_read_length=75 processed_max_read_length=75
2018-10-16 01:19:25 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.preprocessReads_single_end.8cb5bb8a8c4/results/test_se.summary_preprocess.tab
2018-10-16 01:19:25 INFO::preprocessReads.R/mergePreprocessedReads: merging file= /tmp/Rtmp9OnOUL/test.preprocessReads_single_end.8cb5bb8a8c4/bams/processed.aligner_input_1.fastq ...
2018-10-16 01:19:25 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= /tmp/Rtmp9OnOUL/test.preprocessReads_single_end.8cb5bb8a8c4/reports/shortReadReport_1 ...
2018-10-16 01:19:26 INFO::preprocessReads.R/preprocessReads: done
done successfully.
Executing test function test.readRNASeqEnds ... done successfully.
Executing test function test.readRNASeqEnds.dupmark ... done successfully.
Executing test function test.how_many ... done successfully.
Executing test function test.plotDF ... done successfully.
Executing test function test.relativeBarPlot ... done successfully.
Executing test function test.runPipeline ... checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:19:28 INFO::preprocessReads.R/preprocessReads: starting...
2018-10-16 01:19:28 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/extdata/H1993_TP53_subset2500_1.fastq.gz
2018-10-16 01:19:28 INFO::io.R/FastQStreamer.init: initialised FastQ streamer for filename= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/extdata/H1993_TP53_subset2500_2.fastq.gz
2018-10-16 01:19:28 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:19:30 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:19:30 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= test/chunks/chunk_000001/logs/progress.log
2018-10-16 01:19:32 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.046 minutes
2018-10-16 01:19:32 DEBUG::tools.R/processChunks: done
2018-10-16 01:19:32 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_1 ...
2018-10-16 01:19:32 INFO::io.R/saveWithID: saving file= test/results/test.adapter_contaminated_1.RData
2018-10-16 01:19:32 INFO::detectAdapterContam.R/mergeDetectAdapterContam: merging file= adapter_contaminated_2 ...
2018-10-16 01:19:32 INFO::io.R/saveWithID: saving file= test/results/test.adapter_contaminated_2.RData
2018-10-16 01:19:32 INFO::preprocessReads.R/mergeSummaryPreprocess: total_reads=2500 highqual_reads=2500 adapter_contam=0 read_length=100 rRNA_contam_reads=0 processed_reads=2500 input_min_read_length=100 input_max_read_length=100 processed_min_read_length=71 processed_max_read_length=100
2018-10-16 01:19:32 INFO::io.R/saveWithID: saving file= test/results/test.summary_preprocess.tab
2018-10-16 01:19:32 INFO::preprocessReads.R/mergePreprocessedReads: merging file= test/bams/processed.aligner_input_1.fastq ...
2018-10-16 01:19:33 INFO::preprocessReads.R/mergePreprocessedReads: merging file= test/bams/processed.aligner_input_2.fastq ...
2018-10-16 01:19:33 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= test/reports/shortReadReport_1 ...
2018-10-16 01:19:34 INFO::preprocessReads.R/buildShortReadReports: generating report_dir= test/reports/shortReadReport_2 ...
2018-10-16 01:19:36 INFO::preprocessReads.R/preprocessReads: done
2018-10-16 01:19:36 INFO::alignReads.R/alignReads: starting alignment...
2018-10-16 01:19:36 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:19:38 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:19:38 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= test/chunks/chunk_000001/logs/progress.log
2018-10-16 01:19:42 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.076 minutes
2018-10-16 01:19:42 DEBUG::tools.R/processChunks: done
2018-10-16 01:19:42 INFO::alignReads.R/mergeBAMsAcrossDirs: starting...
2018-10-16 01:19:42 INFO::alignReads.R/mergeBAMsAcrossDirs: done
2018-10-16 01:19:42 INFO::io.R/saveWithID: saving file= test/results/test.summary_alignment.tab
2018-10-16 01:19:42 INFO::io.R/saveWithID: saving file= test/results/test.summary_analyzed_bamstats.tab
2018-10-16 01:19:42 INFO::reportPipelineQA.R/writePreprocessAlignReport: creating joint preprocess-alignment report...
2018-10-16 01:19:42 INFO::io.R/saveWithID: saving file= test/results/test.summary_target_lengths.tab
2018-10-16 01:19:43 INFO::reportPipelineQA.R/writePreprocessAlignReport: done
2018-10-16 01:19:43 INFO::alignReads.R/alignReads: done
2018-10-16 01:19:43 INFO::countGenomicFeatures.R/countGenomicFeatures: starting...
2018-10-16 01:19:43 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:19:45 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:19:45 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= test/chunks/chunk_000001/logs/progress.log
2018-10-16 01:19:47 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.041 minutes
2018-10-16 01:19:47 DEBUG::tools.R/processChunks: done
2018-10-16 01:19:47 INFO::countGenomicFeatures.R/mergeCounts: starting...
2018-10-16 01:19:47 INFO::io.R/saveWithID: saving file= test/results/test.counts_exon.tab
2018-10-16 01:19:47 INFO::io.R/saveWithID: saving file= test/results/test.counts_exon_disjoint.tab
2018-10-16 01:19:47 INFO::io.R/saveWithID: saving file= test/results/test.counts_gene.tab
2018-10-16 01:19:47 INFO::io.R/saveWithID: saving file= test/results/test.counts_gene_coding.tab
2018-10-16 01:19:47 INFO::io.R/saveWithID: saving file= test/results/test.counts_gene_exonic.tab
2018-10-16 01:19:47 INFO::io.R/saveWithID: saving file= test/results/test.counts_intergenic.tab
2018-10-16 01:19:47 INFO::io.R/saveWithID: saving file= test/results/test.counts_intron.tab
2018-10-16 01:19:47 INFO::countGenomicFeatures.R/mergeCounts: done
2018-10-16 01:19:47 INFO::io.R/saveWithID: saving file= test/results/test.summary_counts.tab
2018-10-16 01:19:47 INFO::reportPipelineQA.R/writeGenomicFeaturesReport: creating report of genomic features counts...
2018-10-16 01:19:48 INFO::reportPipelineQA.R/writeGenomicFeaturesReport: done
2018-10-16 01:19:48 INFO::countGenomicFeatures.R/countGenomicFeatures: done...
2018-10-16 01:19:48 INFO::coverage.R/calculateCoverage: starting...
2018-10-16 01:19:48 DEBUG::tools.R/processChunks: starting...
2018-10-16 01:19:50 DEBUG::tools.R/processChunks: waiting for chunkid=[ ] ...
2018-10-16 01:19:50 DEBUG::tools.R/processChunks: starting chunkid= 1 ; see logfile= test/chunks/chunk_000001/logs/progress.log
2018-10-16 01:19:52 DEBUG::tools.R/processChunks: done with chunkid= 1 ; elapsed.time= 0.038 minutes
2018-10-16 01:19:52 DEBUG::tools.R/processChunks: done
2018-10-16 01:19:53 INFO::io.R/saveWithID: saving file= test/results/test.coverage.RData
2018-10-16 01:19:53 INFO::coverage.R/saveCoverage: saving file= test/results/test.coverage.bw
2018-10-16 01:19:53 INFO::io.R/saveWithID: saving file= test/results/test.summary_coverage.tab
2018-10-16 01:19:53 INFO::coverage.R/calculateCoverage: done
2018-10-16 01:19:53 INFO::analyzeVariants/analyzeVariants: starting ...
2018-10-16 01:19:53 INFO::analyzeVariants.R/wrap.callVariants: Tallying variants...
2018-10-16 01:19:56 INFO::analyzeVariants.R/wrap.callVariants: calling variants...
2018-10-16 01:19:56 INFO::analyzeVariants.R/wrap.postFilerVariants: post filtering variants...
2018-10-16 01:19:56 INFO::analyzeVariants.R/wrap.callVariants: Saving GRanges of raw and filtered variants...
2018-10-16 01:19:56 INFO::io.R/saveWithID: saving file= test/results/test.raw_variants.RData
2018-10-16 01:19:56 INFO::io.R/saveWithID: saving file= test/results/test.filtered_variants.RData
2018-10-16 01:19:56 INFO::analyzeVariants.R/wrap.callVariants: ...done
2018-10-16 01:19:56 INFO::analyzeVariants.R/writeVCF: writing vcf file...
2018-10-16 01:19:56 INFO::analyzeVariants.R/writeVCF: ...done
2018-10-16 01:19:57 INFO::io.R/saveWithID: saving file= test/results/test.summary_variants.tab
2018-10-16 01:19:57 INFO::analyzeVariants/analyzeVariants: done
2018-10-16 01:19:57 INFO::Pipeline run successful.
done successfully.
Executing test function test.calculateTargetLength ... checkConfig.R/checkConfig.template: loading template config= //tmp/Rtmp9OnOUL/tp53-config.txt
checkConfig.R/checkConfig.template: loading template config= /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/config/default-config.txt
2018-10-16 01:19:57 INFO::io.R/saveWithID: saving file= /tmp/Rtmp9OnOUL/test.calcTargetLengths.8cb6cc5bbdf/results/test_pe.summary_target_lengths.tab
done successfully.
Executing test function test.sclapply ... done successfully.
Executing test function test.tryKeepTraceback ... done successfully.
Executing test function test.truncateReads ... done successfully.
Executing test function test.truncateReads.trim5 ... done successfully.
RUNIT TEST PROTOCOL -- Tue Oct 16 01:20:12 2018
***********************************************
Number of test functions: 62
Number of deactivated test functions: 10
Number of errors: 0
Number of failures: 0
1 Test Suite :
HTSeqGenie unit testing - 62 test functions, 0 errors, 0 failures
Details
***************************
Test Suite: HTSeqGenie unit testing
Test function regexp: ^test.+
Test file regexp: ^runit.+\.[rR]$
Involved directory:
/home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.alignReads.R
test.alignReads: (2 checks) ... OK (27.64 seconds)
test.alignReads.sparsechunks: (2 checks) ... OK (16.28 seconds)
test.alignReadsOneSingleEnd: (2 checks) ... OK (0.9 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.analyzeVariants.R
test.annotateVariants : DEACTIVATED, Skipped annotateVariants() test
test.callVariantsVariantTools.genotype: (2 checks) ... OK (62.06 seconds)
test.wrap.callVariants: (4 checks) ... OK (4.94 seconds)
test.wrap.callVariants.filters: (2 checks) ... OK (7.65 seconds)
test.wrap.callVariants.parallel: (1 checks) ... OK (4.74 seconds)
test.wrap.callVariants.which: (1 checks) ... OK (2.61 seconds)
test.writeVCF.NULL: (1 checks) ... OK (0.68 seconds)
test.writeVCF.vcfStat: (4 checks) ... OK (1.01 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.bamUtils.R
test.isFirstFragment: (5 checks) ... OK (0 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.buildGenomicFeatures.R
test.buildCountsGRangesList: (3 checks) ... OK (33.01 seconds)
test.generateSingleGeneDERs: (4 checks) ... OK (24.29 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.calculateCoverage.R
test.computeCoverage: (9 checks) ... OK (0.67 seconds)
test.isSparse: (5 checks) ... OK (0.07 seconds)
test.mergeCoverage: (1 checks) ... OK (0.91 seconds)
test.mergeCoverage.sparse: (2 checks) ... OK (0.84 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.checkConfig.R
test.checkConfig.analyzeVariants: (5 checks) ... OK (0.73 seconds)
test.findTemplate: (5 checks) ... OK (0.01 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.config.R
test.checkConfig: (5 checks) ... OK (0.99 seconds)
test.checkConfig.alignReads: (5 checks) ... OK (1.47 seconds)
test.getConfig: (14 checks) ... OK (0 seconds)
test.loadConfig: (2 checks) ... OK (0 seconds)
test.parseDCF: (7 checks) ... OK (0 seconds)
test.updateConfig: (1 checks) ... OK (0 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.detectAdapterContam.R
test.getAdapterSeqs: (5 checks) ... OK (0.07 seconds)
test.isAdapter: (5 checks) ... OK (0.16 seconds)
test.isAdapter3.primeEnd: (1 checks) ... OK (0.08 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.detectRRNA.R
test.detectRRNA: (1 checks) ... OK (1.03 seconds)
test.detectRRNA.paired_end: (1 checks) ... OK (1.1 seconds)
test.getRRNAIds: (1 checks) ... OK (0.38 seconds)
test.getRRNAIds_random: (1 checks) ... OK (0.29 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.filterQuality.R
test.filterByLength: (2 checks) ... OK (0.08 seconds)
test.isAboveQualityThresh: (3 checks) ... OK (0.06 seconds)
test.trimTailsByQuality: (4 checks) ... OK (0.05 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.gatk.R
test.callVariantsGATK : DEACTIVATED, callVariantsGATK() tests need gatk.path option set
test.callVariantsGATK.withFiltering : DEACTIVATED, callVariantsGATK() tests need gatk.path option set
test.checkGATKJar : DEACTIVATED, checkGATKJar() test needs gatk.path option set
test.excludeVariantsByRegion: (3 checks) ... OK (0.15 seconds)
test.gatk : DEACTIVATED, gatk() tests need gatk.path option set
test.realignIndels : DEACTIVATED, test.realignIndels() tests need gatk.path option set
test.realignIndelsGATK : DEACTIVATED, realignIndelsGATK() tests need gatk.path option set
test.realignIndelsGATK.parallel : DEACTIVATED, realignIndelsGATK() tests need gatk.path option set
test_zipUp: (3 checks) ... OK (0.01 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.io.R
test.FastQStreamer.getReads.pefq: (1 checks) ... OK (0.85 seconds)
test.FastQStreamer.getReads.pefq.subsample: (1 checks) ... OK (0.88 seconds)
test.FastQStreamer.getReads.segz: (1 checks) ... OK (0.82 seconds)
test.FastQStreamer.getReads.truncated: (1 checks) ... OK (0.7 seconds)
test.FastQStreamer.subsampler.isdeterministic: (3 checks) ... OK (1.62 seconds)
test.createTmpDir: (4 checks) ... OK (0 seconds)
test.detectQualityInFASTQFile: (3 checks) ... OK (0.1 seconds)
test.getObjectFilename: (4 checks) ... OK (0.09 seconds)
test.safeUnlink: (2 checks) ... OK (0.15 seconds)
test.writeAudit: (0 checks) ... OK (1.2 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.mergeLanes.R
test.mergeLanes: (6 checks) ... OK (50.12 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.picard.R
test.markDuplicates : DEACTIVATED, Skipped markDuplicates() test
test.markDuplicates_w_outfile : DEACTIVATED, Skipped markDuplicates() test
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.preprocessReads.R
test.preprocessReads: (5 checks) ... OK (8.33 seconds)
test.preprocessReads.minichunks: (5 checks) ... OK (13.96 seconds)
test.preprocessReads_single_end: (5 checks) ... OK (6.8 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.readRNASeqEnds.R
test.readRNASeqEnds: (6 checks) ... OK (0.11 seconds)
test.readRNASeqEnds.dupmark: (2 checks) ... OK (0.1 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.reportPipelineQA.R
test.how_many: (3 checks) ... OK (0 seconds)
test.plotDF: (3 checks) ... OK (0.17 seconds)
test.relativeBarPlot: (2 checks) ... OK (0.04 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.runPipeline.R
test.runPipeline: (1 checks) ... OK (29.68 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.targetLengths.R
test.calculateTargetLength: (4 checks) ... OK (0.78 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.tools.R
test.sclapply: (10 checks) ... OK (14.36 seconds)
test.tryKeepTraceback: (3 checks) ... OK (0.01 seconds)
---------------------------
Test file: /home/biocbuild/bbs-3.7-bioc/R/library/HTSeqGenie/unitTests/runit.trimReads.R
test.truncateReads: (7 checks) ... OK (0.08 seconds)
test.truncateReads.trim5: (15 checks) ... OK (0.25 seconds)
runTests.R: OK !
There were 50 or more warnings (use warnings() to see the first 50)
>
> proc.time()
user system elapsed
204.716 75.664 337.737