##############################################################################
##############################################################################
###
### Running command:
###
###   /Library/Frameworks/R.framework/Resources/bin/R CMD check --install=check:multiWGCNA.install-out.txt --library=/Library/Frameworks/R.framework/Resources/library --no-vignettes --timings multiWGCNA_1.9.1.tar.gz
###
##############################################################################
##############################################################################


* using log directory ‘/Users/biocbuild/bbs-3.23-bioc/meat/multiWGCNA.Rcheck’
* using R Under development (unstable) (2025-11-04 r88984)
* using platform: aarch64-apple-darwin20
* R was compiled by
    Apple clang version 16.0.0 (clang-1600.0.26.6)
    GNU Fortran (GCC) 14.2.0
* running under: macOS Ventura 13.7.8
* using session charset: UTF-8
* using option ‘--no-vignettes’
* checking for file ‘multiWGCNA/DESCRIPTION’ ... OK
* checking extension type ... Package
* this is package ‘multiWGCNA’ version ‘1.9.1’
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘multiWGCNA’ can be installed ... OK
* checking installed package size ... OK
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking code files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... NOTE
BuildTOMFlowDF: no visible global function definition for ‘as.dist’
BuildTOMFlowDF: no visible binding for global variable ‘Gene’
BuildTOMFlowDF: no visible binding for global variable ‘Network’
BuildTOMFlowDF: no visible binding for global variable ‘Label’
BuildTOMFlowDF: no visible binding for global variable ‘Order’
BuildTOMFlowDF: no visible binding for global variable ‘Color’
GetSignificantOverlap: no visible binding for global variable ‘p.adj’
GetSignificantOverlap: no visible binding for global variable ‘overlap’
ModuleFlowPlot : <anonymous>: no visible binding for global variable
  ‘mod1’
ModuleFlowPlot : <anonymous>: no visible binding for global variable
  ‘mod2’
ModuleFlowPlot: no visible binding for global variable ‘module’
ModuleFlowPlot: no visible binding for global variable ‘p.adj’
ModuleFlowPlot: no visible binding for global variable ‘overlap’
ModuleFlowPlot: no visible binding for global variable ‘n.genes’
ModuleFlowPlot: no visible binding for global variable ‘color’
ModuleFlowPlot: no visible binding for global variable ‘name_clean’
ModuleFlowPlot: no visible binding for global variable ‘x’
ModuleFlowPlot: no visible binding for global variable ‘y’
ModuleFlowPlot: no visible binding for global variable ‘text’
ModuleFlowPlot2Way: no visible binding for global variable ‘overlap’
ModuleFlowPlot2Way: no visible binding for global variable ‘mod1’
ModuleFlowPlot2Way: no visible binding for global variable ‘mod2’
ModuleFlowPlot2Way: no visible binding for global variable ‘p.adj’
ModuleFlowPlot2Way: no visible binding for global variable ‘stratum’
PlotMultiNodesTOMflow: no visible binding for global variable ‘module’
PlotMultiNodesTOMflow: no visible binding for global variable ‘Count’
PlotMultiNodesTOMflow: no visible binding for global variable ‘stratum’
TOMFlowPlot: no visible global function definition for ‘as.dist’
TOMFlowPlot: no visible binding for global variable ‘module’
TOMFlowPlot: no visible binding for global variable ‘Count’
TOMFlowPlot: no visible binding for global variable ‘one.gene’
TOMFlowPlot: no visible binding for global variable ‘two.gene’
TOMFlowPlot: no visible binding for global variable ‘stratum’
bidirectionalBestMatches: no visible binding for global variable ‘mod1’
bidirectionalBestMatches: no visible binding for global variable ‘mod2’
bidirectionalBestMatches: no visible binding for global variable
  ‘overlap’
coexpressionLineGraph: no visible binding for global variable ‘Var1’
coexpressionLineGraph: no visible binding for global variable ‘value’
coexpressionLineGraph: no visible binding for global variable ‘Var2’
computeOverlapsFromWGCNA: no visible binding for global variable ‘mod1’
computeOverlapsFromWGCNA: no visible binding for global variable ‘mod2’
continuousFlowPlot: no visible global function definition for ‘ulist’
continuousFlowPlot: no visible binding for global variable
  ‘uniqueSortedData’
continuousFlowPlot: no visible binding for global variable ‘stratum’
continuousFlowPlot: no visible global function definition for ‘error’
correlationComparisonBoxplot: no visible binding for global variable
  ‘Status’
correlationComparisonBoxplot: no visible binding for global variable
  ‘Correlation’
correlationComparisonHeatmaps: no visible binding for global variable
  ‘Var1’
correlationComparisonHeatmaps: no visible binding for global variable
  ‘Var2’
correlationComparisonHeatmaps: no visible binding for global variable
  ‘value’
diffCoexpression: no visible binding for global variable ‘p.adj’
diffModuleExpression: no visible binding for global variable ‘Sample’
expressionHeatmap: no visible binding for global variable ‘Sample’
expressionHeatmap: no visible binding for global variable ‘Gene’
expressionHeatmap: no visible binding for global variable ‘Zscore’
findOutlierModules: no visible global function definition for
  ‘zScoreMatrix’
getPreservation: no visible binding for global variable ‘name2’
makeTraitTable2: no visible global function definition for
  ‘model.matrix’
moduleComparisonPlot: no visible binding for global variable ‘overlap’
moduleComparisonPlot: no visible binding for global variable ‘mod1’
moduleComparisonPlot: no visible binding for global variable ‘mod2’
moduleComparisonPlot: no visible binding for global variable ‘p.adj’
moduleComparisonPlot: no visible binding for global variable ‘stratum’
moduleExpressionPlot: no visible binding for global variable ‘Sample’
moduleExpressionPlot: no visible binding for global variable
  ‘Expression’
moduleToModuleHeatmap: no visible binding for global variable ‘mod1’
moduleToModuleHeatmap: no visible binding for global variable ‘mod2’
moduleToModuleHeatmap: no visible binding for global variable ‘p.adj’
moduleToModuleHeatmap: no visible binding for global variable ‘overlap’
preservationComparisonPlot: no visible binding for global variable
  ‘log10Pvalue’
preservationComparisonPlot: no visible binding for global variable
  ‘Zsum’
preservationComparisonPlot: no visible binding for global variable
  ‘trait’
preservationComparisonPlot: no visible binding for global variable
  ‘Module’
topNGenes: no visible binding for global variable ‘kWithin’
Undefined global functions or variables:
  Color Correlation Count Expression Gene Label Module Network Order
  Sample Status Var1 Var2 Zscore Zsum as.dist color error kWithin
  log10Pvalue mod1 mod2 model.matrix module n.genes name2 name_clean
  one.gene overlap p.adj stratum text trait two.gene ulist
  uniqueSortedData value x y zScoreMatrix
Consider adding
  importFrom("graphics", "text")
  importFrom("stats", "as.dist", "model.matrix")
to your NAMESPACE file.
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking contents of ‘data’ directory ... OK
* checking data for non-ASCII characters ... OK
* checking LazyData ... OK
* checking data for ASCII and uncompressed saves ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... ERROR
Running examples in ‘multiWGCNA-Ex.R’ failed
The error most likely occurred in:

> base::assign(".ptime", proc.time(), pos = "CheckExEnv")
> ### Name: diffCoexpression
> ### Title: Differential co-expresison analysis
> ### Aliases: diffCoexpression
> 
> ### ** Examples
> 
> library(ExperimentHub)
Loading required package: BiocGenerics
Loading required package: generics

Attaching package: ‘generics’

The following objects are masked from ‘package:base’:

    as.difftime, as.factor, as.ordered, intersect, is.element, setdiff,
    setequal, union


Attaching package: ‘BiocGenerics’

The following objects are masked from ‘package:stats’:

    IQR, mad, sd, var, xtabs

The following objects are masked from ‘package:base’:

    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, is.unsorted, lapply,
    mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int,
    rank, rbind, rownames, sapply, saveRDS, table, tapply, unique,
    unsplit, which.max, which.min

Loading required package: AnnotationHub
Loading required package: BiocFileCache
Loading required package: dbplyr
> eh = ExperimentHub()
> eh_query = query(eh, c("multiWGCNAdata"))
> astrocyte_se = eh_query[["EH8223"]]
multiWGCNAdata not installed.
  Full functionality, documentation, and loading of data might not be possible without installing
loading from cache
require(“SummarizedExperiment”)
> datExpr = assays(astrocyte_se)[[1]]
> diffCoexpression(datExpr, c(rep(1,20), rep(2,16)), 
+   geneList = c("Gfap", "Vim", "Aspg", "Serpina3n", "Cp", "Osmr", "Cd44", 
+     "Cxcl10", "Hspb1", "Timp1", "S1pr3", "Steap4", "Lcn2"))
Error in `igraph::graph_from_adjacency_matrix()`:
! Cannot create a graph object because the adjacency matrix contains
  NAs.
Backtrace:
    ▆
 1. └─multiWGCNA::diffCoexpression(...)
 2.   └─igraph::graph_from_adjacency_matrix(adj_mat, mode = "undirected", weighted = TRUE)
 3.     └─igraph:::ensure_no_na(adjmatrix, "adjacency matrix")
 4.       └─cli::cli_abort(...)
 5.         └─rlang::abort(...)
Execution halted
Examples with CPU (user + system) or elapsed time > 5s
                     user system elapsed
constructNetworks  19.248  0.125  20.787
ModuleFlowPlot2Way  4.623  0.126   5.120
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘testthat.R’
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE

Status: 1 ERROR, 1 NOTE
See
  ‘/Users/biocbuild/bbs-3.23-bioc/meat/multiWGCNA.Rcheck/00check.log’
for details.

##############################################################################
##############################################################################
###
### Running command:
###
###   /Library/Frameworks/R.framework/Resources/bin/R CMD INSTALL multiWGCNA
###
##############################################################################
##############################################################################


* installing to library ‘/Library/Frameworks/R.framework/Versions/4.6-arm64/Resources/library’
* installing *source* package ‘multiWGCNA’ ...
** this is package ‘multiWGCNA’ version ‘1.9.1’
** using staged installation
** R
** data
*** moving datasets to lazyload DB
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (multiWGCNA)

R Under development (unstable) (2025-11-04 r88984) -- "Unsuffered Consequences"
Copyright (C) 2025 The R Foundation for Statistical Computing
Platform: aarch64-apple-darwin20

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> # This file is part of the standard setup for testthat.
> # It is recommended that you do not modify it.
> #
> # Where should you do additional test configuration?
> # Learn more about the roles of various files in:
> # * https://r-pkgs.org/tests.html
> # * https://testthat.r-lib.org/reference/test_package.html#special-files
> 
> library(testthat)
> library(multiWGCNA)
Loading required package: ggalluvial
Loading required package: ggplot2

> 
> test_check("multiWGCNA")
Loading required package: BiocGenerics
Loading required package: generics

Attaching package: 'generics'

The following objects are masked from 'package:base':

    as.difftime, as.factor, as.ordered, intersect, is.element, setdiff,
    setequal, union


Attaching package: 'BiocGenerics'

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, is.unsorted, lapply,
    mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int,
    rank, rbind, rownames, sapply, saveRDS, table, tapply, unique,
    unsplit, which.max, which.min

Loading required package: AnnotationHub
Loading required package: BiocFileCache
Loading required package: dbplyr
multiWGCNAdata not installed.
  Full functionality, documentation, and loading of data might not be possible without installing
loading from cache
require("SummarizedExperiment")
### Working on combined network ###
 Flagging genes and samples with too many missing values...
  ..step 1
 Calculating module eigengenes block-wise from all genes
   Flagging genes and samples with too many missing values...
    ..step 1
 ..Working on block 1 .
    TOM calculation: adjacency..
    ..will not use multithreading.
     Fraction of slow calculations: 0.000000
    ..connectivity..
    ..matrix multiplication (system BLAS)..
    ..normalization..
    ..done.
 ....clustering..
 ....detecting modules..
 ....calculating module eigengenes..
 ....checking kME in modules..
     ..removing 163 genes from module 1 because their KME is too low.
     ..removing 126 genes from module 2 because their KME is too low.
     ..removing 59 genes from module 3 because their KME is too low.
     ..removing 18 genes from module 4 because their KME is too low.
     ..removing 28 genes from module 5 because their KME is too low.
     ..removing 24 genes from module 6 because their KME is too low.
     ..removing 9 genes from module 7 because their KME is too low.
 ..merging modules that are too close..
     mergeCloseModules: Merging modules whose distance is less than 0.1
       Calculating new MEs...
 softConnectivity: FYI: connecitivty of genes with less than 20 valid samples will be returned as NA.
 ..calculating connectivities.. 
### Working on autism network ###
 Flagging genes and samples with too many missing values...
  ..step 1
 Calculating module eigengenes block-wise from all genes
   Flagging genes and samples with too many missing values...
    ..step 1
 ..Working on block 1 .
    TOM calculation: adjacency..
    ..will not use multithreading.
     Fraction of slow calculations: 0.000000
    ..connectivity..
    ..matrix multiplication (system BLAS)..
    ..normalization..
    ..done.
 ....clustering..
 ....detecting modules..
 ....calculating module eigengenes..
 ....checking kME in modules..
     ..removing 139 genes from module 1 because their KME is too low.
     ..removing 82 genes from module 2 because their KME is too low.
     ..removing 95 genes from module 3 because their KME is too low.
     ..removing 37 genes from module 4 because their KME is too low.
     ..removing 35 genes from module 5 because their KME is too low.
     ..removing 36 genes from module 6 because their KME is too low.
     ..removing 27 genes from module 7 because their KME is too low.
 ..merging modules that are too close..
     mergeCloseModules: Merging modules whose distance is less than 0.1
       Calculating new MEs...
 softConnectivity: FYI: connecitivty of genes with less than 10 valid samples will be returned as NA.
 ..calculating connectivities.. 
### Working on FC network ###
 Flagging genes and samples with too many missing values...
  ..step 1
 Calculating module eigengenes block-wise from all genes
   Flagging genes and samples with too many missing values...
    ..step 1
 ..Working on block 1 .
    TOM calculation: adjacency..
    ..will not use multithreading.
     Fraction of slow calculations: 0.000000
    ..connectivity..
    ..matrix multiplication (system BLAS)..
    ..normalization..
    ..done.
 ....clustering..
 ....detecting modules..
 ....calculating module eigengenes..
 ....checking kME in modules..
     ..removing 164 genes from module 1 because their KME is too low.
     ..removing 138 genes from module 2 because their KME is too low.
     ..removing 86 genes from module 3 because their KME is too low.
     ..removing 33 genes from module 4 because their KME is too low.
     ..removing 35 genes from module 5 because their KME is too low.
     ..removing 34 genes from module 6 because their KME is too low.
     ..removing 19 genes from module 7 because their KME is too low.
 ..merging modules that are too close..
     mergeCloseModules: Merging modules whose distance is less than 0.1
       Calculating new MEs...
 softConnectivity: FYI: connecitivty of genes with less than 11 valid samples will be returned as NA.
 ..calculating connectivities.. 
### Working on combined network ###
 Flagging genes and samples with too many missing values...
  ..step 1
 Calculating module eigengenes block-wise from all genes
   Flagging genes and samples with too many missing values...
    ..step 1
 ..Working on block 1 .
    TOM calculation: adjacency..
    ..will not use multithreading.
     Fraction of slow calculations: 0.000000
    ..connectivity..
    ..matrix multiplication (system BLAS)..
    ..normalization..
    ..done.
 ....clustering..
 ....detecting modules..
 ....calculating module eigengenes..
 ....checking kME in modules..
     ..removing 163 genes from module 1 because their KME is too low.
     ..removing 126 genes from module 2 because their KME is too low.
     ..removing 59 genes from module 3 because their KME is too low.
     ..removing 18 genes from module 4 because their KME is too low.
     ..removing 28 genes from module 5 because their KME is too low.
     ..removing 24 genes from module 6 because their KME is too low.
     ..removing 9 genes from module 7 because their KME is too low.
 ..merging modules that are too close..
     mergeCloseModules: Merging modules whose distance is less than 0.1
       Calculating new MEs...
 softConnectivity: FYI: connecitivty of genes with less than 20 valid samples will be returned as NA.
 ..calculating connectivities.. 
### Working on autism network ###
 Flagging genes and samples with too many missing values...
  ..step 1
 Calculating module eigengenes block-wise from all genes
   Flagging genes and samples with too many missing values...
    ..step 1
 ..Working on block 1 .
    TOM calculation: adjacency..
    ..will not use multithreading.
     Fraction of slow calculations: 0.000000
    ..connectivity..
    ..matrix multiplication (system BLAS)..
    ..normalization..
    ..done.
 ....clustering..
 ....detecting modules..
 ....calculating module eigengenes..
 ....checking kME in modules..
     ..removing 139 genes from module 1 because their KME is too low.
     ..removing 82 genes from module 2 because their KME is too low.
     ..removing 95 genes from module 3 because their KME is too low.
     ..removing 37 genes from module 4 because their KME is too low.
     ..removing 35 genes from module 5 because their KME is too low.
     ..removing 36 genes from module 6 because their KME is too low.
     ..removing 27 genes from module 7 because their KME is too low.
 ..merging modules that are too close..
     mergeCloseModules: Merging modules whose distance is less than 0.1
       Calculating new MEs...
 softConnectivity: FYI: connecitivty of genes with less than 10 valid samples will be returned as NA.
 ..calculating connectivities.. 
### Working on FC network ###
 Flagging genes and samples with too many missing values...
  ..step 1
 Calculating module eigengenes block-wise from all genes
   Flagging genes and samples with too many missing values...
    ..step 1
 ..Working on block 1 .
    TOM calculation: adjacency..
    ..will not use multithreading.
     Fraction of slow calculations: 0.000000
    ..connectivity..
    ..matrix multiplication (system BLAS)..
    ..normalization..
    ..done.
 ....clustering..
 ....detecting modules..
 ....calculating module eigengenes..
 ....checking kME in modules..
     ..removing 164 genes from module 1 because their KME is too low.
     ..removing 138 genes from module 2 because their KME is too low.
     ..removing 86 genes from module 3 because their KME is too low.
     ..removing 33 genes from module 4 because their KME is too low.
     ..removing 35 genes from module 5 because their KME is too low.
     ..removing 34 genes from module 6 because their KME is too low.
     ..removing 19 genes from module 7 because their KME is too low.
 ..merging modules that are too close..
     mergeCloseModules: Merging modules whose distance is less than 0.1
       Calculating new MEs...
 softConnectivity: FYI: connecitivty of genes with less than 11 valid samples will be returned as NA.
 ..calculating connectivities.. 
[ FAIL 0 | WARN 0 | SKIP 0 | PASS 2 ]
> 
> proc.time()
   user  system elapsed 
 42.808   0.474  46.240