This page was generated on 2023-01-02 09:00:52 -0500 (Mon, 02 Jan 2023).
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palomino5 | Windows Server 2022 Datacenter | x64 | R Under development (unstable) (2022-12-25 r83502 ucrt) -- "Unsuffered Consequences"
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CHECK results for SpliceWiz on palomino5
To the developers/maintainers of the SpliceWiz package:
Make sure to use the following settings in order to reproduce any error or warning you see on this page.
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raw results
| SpliceWiz 1.1.5 (landing page) Alex Chit Hei Wong
Snapshot Date: 2022-12-28 11:00:06 -0500 (Wed, 28 Dec 2022) |
git_url: https://git.bioconductor.org/packages/SpliceWiz |
git_branch: master |
git_last_commit: 79fa481 |
git_last_commit_date: 2022-12-19 18:37:31 -0500 (Mon, 19 Dec 2022) |
| palomino5 | Windows Server 2022 Datacenter / x64 | OK | OK | OK | OK | | |
Summary
Command output
Installation output
SpliceWiz.Rcheck/00install.out
Tests output
SpliceWiz.Rcheck/tests/testthat.Rout
R Under development (unstable) (2022-12-25 r83502 ucrt) -- "Unsuffered Consequences"
Copyright (C) 2022 The R Foundation for Statistical Computing
Platform: x86_64-w64-mingw32/x64 (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(testthat)
> library(SpliceWiz)
Loading required package: NxtIRFdata
SpliceWiz package loaded with 2 threads
Use setSWthreads() to set the number of SpliceWiz threads
>
> test_check("SpliceWiz")
Loading required package: Rsubread
Dec 29 3:58:10 AM Converting FASTA to local TwoBitFile...done
Dec 29 3:58:11 AM Connecting to genome TwoBitFile...done
Dec 29 3:58:11 AM Making local copy of GTF file...done
Dec 29 3:58:12 AM Extracting temp genome FASTA from TwoBit file
Dec 29 3:58:13 AM Generating synthetic reads, saving to F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\refWithMapExcl/Mappability/Reads.fa
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
10145 synthetic reads generated
Dec 29 3:58:13 AM Cleaning temp genome / gene annotation files
========== _____ _ _ ____ _____ ______ _____
===== / ____| | | | _ \| __ \| ____| /\ | __ \
===== | (___ | | | | |_) | |__) | |__ / \ | | | |
==== \___ \| | | | _ <| _ /| __| / /\ \ | | | |
==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| |
========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/
Rsubread 2.13.2
//================================= setting ==================================\\
|| ||
|| Index name : reference_index ||
|| Index space : base space ||
|| Index split : no-split ||
|| Repeat threshold : 100 repeats ||
|| Gapped index : no ||
|| ||
|| Free / total memory : 42.5GB / 64.0GB ||
|| ||
|| Input files : 1 file in total ||
|| o genome.fa ||
|| ||
\\============================================================================//
//================================= Running ==================================\\
|| ||
|| Check the integrity of provided reference sequences ... ||
|| No format issues were found ||
|| Scan uninformative subreads in reference sequences ... ||
|| Estimate the index size... ||
|| 8%, 0 mins elapsed, rate=94.7k bps/s ||
|| 16%, 0 mins elapsed, rate=184.7k bps/s ||
|| 24%, 0 mins elapsed, rate=270.5k bps/s ||
|| 33%, 0 mins elapsed, rate=352.8k bps/s ||
|| 41%, 0 mins elapsed, rate=432.0k bps/s ||
|| 49%, 0 mins elapsed, rate=508.1k bps/s ||
|| 58%, 0 mins elapsed, rate=581.0k bps/s ||
|| 66%, 0 mins elapsed, rate=650.9k bps/s ||
|| 74%, 0 mins elapsed, rate=718.8k bps/s ||
|| 83%, 0 mins elapsed, rate=784.9k bps/s ||
|| 91%, 0 mins elapsed, rate=847.8k bps/s ||
|| 3.0 GB of memory is needed for index building. ||
|| Build the index... ||
|| 8%, 0 mins elapsed, rate=8.5k bps/s ||
|| 16%, 0 mins elapsed, rate=17.0k bps/s ||
|| 24%, 0 mins elapsed, rate=25.4k bps/s ||
|| 33%, 0 mins elapsed, rate=33.7k bps/s ||
|| 41%, 0 mins elapsed, rate=42.0k bps/s ||
|| 49%, 0 mins elapsed, rate=50.3k bps/s ||
|| 58%, 0 mins elapsed, rate=58.5k bps/s ||
|| 66%, 0 mins elapsed, rate=66.6k bps/s ||
|| 74%, 0 mins elapsed, rate=74.8k bps/s ||
|| 83%, 0 mins elapsed, rate=82.8k bps/s ||
|| 91%, 0 mins elapsed, rate=90.9k bps/s ||
|| Save current index block... ||
|| [ 0.0% finished ] ||
|| [ 10.0% finished ] ||
|| [ 20.0% finished ] ||
|| [ 30.0% finished ] ||
|| [ 40.0% finished ] ||
|| [ 50.0% finished ] ||
|| [ 60.0% finished ] ||
|| [ 70.0% finished ] ||
|| [ 80.0% finished ] ||
|| [ 90.0% finished ] ||
|| [ 100.0% finished ] ||
|| ||
|| Total running time: 0.2 minutes. ||
||Index F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\refWithMap ... ||
|| ||
\\============================================================================//
========== _____ _ _ ____ _____ ______ _____
===== / ____| | | | _ \| __ \| ____| /\ | __ \
===== | (___ | | | | |_) | |__) | |__ / \ | | | |
==== \___ \| | | | _ <| _ /| __| / /\ \ | | | |
==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| |
========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/
Rsubread 2.13.2
//================================= setting ==================================\\
|| ||
|| Function : Read alignment + Junction detection (RNA-Seq) ||
|| Input file : Reads.fa ||
|| Output file : AlignedReads.bam (BAM) ||
|| Index name : reference_index ||
|| ||
|| ------------------------------------ ||
|| ||
|| Threads : 1 ||
|| Phred offset : 33 ||
|| Min votes : 1 / 14 ||
|| Max mismatches : 3 ||
|| Max indel length : 5 ||
|| Report multi-mapping reads : yes ||
|| Max alignments per multi-mapping read : 1 ||
|| Annotations : transcripts.gtf (GTF) ||
|| ||
\\============================================================================//
//================ Running (29-Dec-2022 03:58:23, pid=83636) =================\\
|| ||
|| Check the input reads. ||
|| The input file contains base space reads. ||
|| Initialise the memory objects. ||
|| Estimate the mean read length. ||
|| Create the output BAM file. ||
|| Check the index. ||
|| Init the voting space. ||
|| Load the annotation file. ||
|| 538 annotation records were loaded. ||
|| Global environment is initialised. ||
|| Load the 1-th index block... ||
|| The index block has been loaded. ||
|| Start read mapping in chunk. ||
|| 65% completed, 0.3 mins elapsed, rate=47.9k reads per second ||
|| 83% completed, 0.3 mins elapsed, rate=0.5k reads per second ||
|| 99% completed, 0.3 mins elapsed, rate=0.6k reads per second ||
|| ||
|| Completed successfully. ||
|| ||
\\==================================== ====================================//
//================================ Summary =================================\\
|| ||
|| Total reads : 10145 ||
|| Mapped : 10145 (100.0%) ||
|| Uniquely mapped : 10145 ||
|| Multi-mapping : 0 ||
|| ||
|| Unmapped : 0 ||
|| ||
|| Junctions : 0 ||
|| Indels : 1 ||
|| ||
|| Running time : 0.3 minutes ||
|| ||
\\============================================================================//
Dec 29 3:58:41 AM Calculating Mappability Exclusion regions from: F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0/refWithMapExcl/Mappability/AlignedReads.bam
Calculating Mappability Exclusions: F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\refWithMapExcl\Mappability\AlignedReads.bam
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Performing final sort of fragment maps
Writing Mappability Exclusions
**************************************************|
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Dec 29 3:58:44 AM Reference generated without non-polyA reference
Dec 29 3:58:44 AM Reference generated without Mappability reference
Dec 29 3:58:44 AM Reference generated without Blacklist exclusion
Dec 29 3:58:44 AM Converting FASTA to local TwoBitFile...done
Dec 29 3:58:45 AM Connecting to genome TwoBitFile...done
Dec 29 3:58:45 AM Making local copy of GTF file...done
Dec 29 3:58:46 AM Reading source GTF file...done
Dec 29 3:58:46 AM Processing gtf file...
...genes
...transcripts
...CDS
...exons
done
Dec 29 3:58:48 AM Processing introns...
...data
...basic annotations
...splice motifs
...other info
...defining flanking exon clusters
done
Dec 29 3:58:51 AM Generating processBAM reference
...prepping data
...determining measurable introns (directional)
...determining measurable introns (non-directional)
...writing ref-cover.bed
...writing ref-ROI.bed
...writing ref-read-continues.ref
...writing ref-sj.ref
...writing ref-tj.ref
processBAM reference generated
Dec 29 3:58:57 AM Predicting NMD transcripts from genome sequence
...exonic transcripts
...retained introns
|
| | 0%
|
|======================================================================| 100%
done
Dec 29 3:58:59 AM Annotating Splice Events
Annotating Mutually-Exclusive-Exon Splice Events...done
Annotating Skipped-Exon Splice Events...done
Annotating Alternate 5' / 3' Splice Site Splice Events...done
Annotating Alternate First / Last Exon Splice Events...done
Annotating known retained introns...done
Dec 29 3:59:00 AM Splice Annotations Filtered
Dec 29 3:59:00 AM Translating Alternate Splice Peptides...done
Dec 29 3:59:01 AM Splice Annotations finished
Reference build finished
Dec 29 3:59:01 AM Running SpliceWiz processBAM
Reading reference file
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (0 milliseconds)
Processing BAM file F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H003.bam
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Performing final sort of fragment maps
Writing COV file
**************************************************|
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Writing output file
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H003.bam processed (349 milliseconds)
Dec 29 3:59:02 AM Running SpliceWiz processBAM
Reading reference file
Allocating memory to 2 threads for SpliceWiz (ompBAM)...initialized (1 milliseconds)
Processing BAM file F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H003.bam
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Compiling data from threads
Performing final sort of fragment maps
Writing COV file
**************************************************|
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Writing output file
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H003.bam processed (295 milliseconds)
Dec 29 3:59:02 AM SpliceWiz reference already exists in given directory
Dec 29 3:59:02 AM Running SpliceWiz processBAM
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (0 milliseconds)
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H003.bam processed (343 milliseconds)
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H025.bam processed (277 milliseconds)
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H026.bam processed (415 milliseconds)
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H033.bam processed (420 milliseconds)
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H043.bam processed (279 milliseconds)
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H046.bam processed (415 milliseconds)
Dec 29 3:59:04 AM Validating Experiment; checking COV files...
Dec 29 3:59:04 AM Compiling Sample Stats
Dec 29 3:59:04 AM Compiling Junction List...merging...done
Dec 29 3:59:05 AM Compiling Junction Stats...merging...done
Dec 29 3:59:10 AM Compiling Intron Retention List...done
Dec 29 3:59:11 AM Compiling Tandem Junction List...merging...done
Dec 29 3:59:12 AM Tidying up splice junctions and intron retentions...
...annotating splice junctions
...looking for novel exons
Dec 29 3:59:15 AM Assembling novel splicing reference:
...loading reference FASTA/GTF
...injecting novel transcripts to GTF
...processing GTF
...processing introns from GTF
...annotating alternative splicing events
done
Dec 29 3:59:22 AM Tidying up splice junctions and intron retentions (part 2)...
...grouping splice junctions
...grouping introns
...loading splice events
...compiling rowEvents
done
Dec 29 3:59:27 AM Generating NxtSE assays
Dec 29 3:59:38 AM Saving assays into H5 file
Dec 29 3:59:39 AM Saving auxiliary data
Dec 29 3:59:39 AM Assembling final NxtSE object
Dec 29 3:59:40 AM Saving final NxtSE object
Dec 29 3:59:40 AM SpliceWiz (NxtSE) Collation Finished
Dec 29 3:59:40 AM Loading NxtSE object from file...done
Dec 29 3:59:40 AM Removing overlapping introns...
Dec 29 3:59:40 AM Iterating through IR events to determine introns of main isoforms
Dec 29 3:59:41 AM Iteration 1
Dec 29 3:59:42 AM Iteration 2
Dec 29 3:59:44 AM SpliceWiz reference already exists in given directory
Dec 29 3:59:44 AM Running SpliceWiz processBAM
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (1 milliseconds)
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H003.bam processed (464 milliseconds)
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H025.bam processed (381 milliseconds)
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H026.bam processed (491 milliseconds)
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H033.bam processed (427 milliseconds)
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H043.bam processed (266 milliseconds)
F:\biocbuild\bbs-3.17-bioc-rtools43\tmpdir\RtmpsLxiL0\02H046.bam processed (391 milliseconds)
Dec 29 3:59:46 AM Validating Experiment; checking COV files...
Dec 29 3:59:46 AM Compiling Sample Stats
Dec 29 3:59:46 AM Compiling Junction List...merging...done
Dec 29 3:59:47 AM Compiling Junction Stats...merging...done
Dec 29 3:59:52 AM Compiling Intron Retention List...done
Dec 29 3:59:53 AM Tidying up splice junctions and intron retentions...
...annotating splice junctions
...grouping splice junctions
...grouping introns
...loading splice events
...compiling rowEvents
done
Dec 29 4:00:00 AM Generating NxtSE assays
Dec 29 4:00:11 AM Saving assays into H5 file
Dec 29 4:00:13 AM Saving auxiliary data
Dec 29 4:00:13 AM Assembling final NxtSE object
Dec 29 4:00:13 AM Saving final NxtSE object
Dec 29 4:00:13 AM SpliceWiz (NxtSE) Collation Finished
Dec 29 4:00:13 AM Loading NxtSE object from file...done
Dec 29 4:00:13 AM Removing overlapping introns...
Dec 29 4:00:13 AM Iterating through IR events to determine introns of main isoforms
Dec 29 4:00:14 AM Iteration 1
Dec 29 4:00:15 AM Iteration 2
[ FAIL 0 | WARN 0 | SKIP 0 | PASS 29 ]
>
> # bump to trigger r cmd check
>
> proc.time()
user system elapsed
131.60 5.12 139.28
Example timings
SpliceWiz.Rcheck/SpliceWiz-Ex.timings