## ----setup, include=FALSE----------------------------------------------------- knitr::opts_chunk$set( echo = TRUE, warning = FALSE, message = FALSE, error = FALSE, eval = FALSE, tidy = FALSE, dev = c("png"), cache = TRUE ) ## ----example, eval=FALSE------------------------------------------------------ # library(zellkonverter) # library(SingleCellExperiment) # # # Create SingleCellExperiment object that points to on-disk H5AD file # sce <- readH5AD(h5ad_file, use_hdf5 = TRUE) ## ----example2, eval=FALSE----------------------------------------------------- # # Read a series of H5AD files into a list # # then combine them into a single merged SingleCellExperiment # sce.lst <- lapply(h5ad_files, function(file) { # readH5AD(file, use_hdf5 = TRUE) # }) # sce <- do.call(cbind, sce.list) ## ----example3, eval=FALSE----------------------------------------------------- # # read raw/ from H5AD file # # raw = TRUE tells readH5AD() to read alternative data # # Must use zellkonverter >=1.3.3 # sce_in <- readH5AD(h5ad_file, use_hdf5 = TRUE, raw = TRUE) # # # use `raw` as counts # sce <- swapAltExp(sce_in, "raw") # use raw as main # counts(sce) <- assay(sce, "X") # set counts assay to data in X # assay(sce, "X") <- NULL # free X ## ----Seurat, eval=FALSE------------------------------------------------------- # library(SingleCellExperiment) # library(SeuratDisk) # # # Convert h5ad file to h5seurat format # Convert(h5ad_file, dest = "h5seurat") # # # load Seurat file # obj <- LoadH5Seurat(h5seurat_file) # # # Convert Seurat object to SingleCellExperiment # sce <- as.SingleCellExperiment(obj) ## ----session, echo=FALSE------------------------------------------------------ # sessionInfo()