crisprVerse: ecosystem of R packages for CRISPR gRNA design

Installation and getting started

The crisprVerse is a collection of packages for CRISPR guide RNA (gRNA) design that can easily be installed with the crisprVerse package. This provides a convenient way of downloading and installing all crisprVerse packages with a single R command.

The package can be installed from the Bioconductor devel branch using the following commands in an R session:

if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install(version="devel")
BiocManager::install("crisprVerse")

The core crisprVerse includes the packages that are commonly used for gRNA design, and are attached when you attach the crisprVerse package:

library(crisprVerse)

You can check that all crisprVerse packages are up-to-date with the function crisprVerse_update().

Components

The following packages are installed and loaded with the crisprVerse package:

Reproducibility

sessionInfo()
## R version 4.6.0 (2026-04-24)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 24.04.4 LTS
## 
## Matrix products: default
## BLAS:   /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3 
## LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.26.so;  LAPACK version 3.12.0
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## time zone: Etc/UTC
## tzcode source: system (glibc)
## 
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
##  [1] crisprViz_1.14.0       crisprDesign_1.14.0    crisprScore_1.16.0    
##  [4] crisprScoreData_1.15.0 ExperimentHub_3.2.0    AnnotationHub_4.2.0   
##  [7] BiocFileCache_3.2.0    dbplyr_2.5.2           BiocGenerics_0.58.0   
## [10] generics_0.1.4         crisprBowtie_1.16.0    crisprBase_1.16.0     
## [13] crisprVerse_1.14.0     BiocStyle_2.40.0      
## 
## loaded via a namespace (and not attached):
##   [1] RColorBrewer_1.1-3          rstudioapi_0.18.0          
##   [3] sys_3.4.3                   jsonlite_2.0.0             
##   [5] magrittr_2.0.5              GenomicFeatures_1.64.0     
##   [7] farver_2.1.2                rmarkdown_2.31             
##   [9] BiocIO_1.22.0               vctrs_0.7.3                
##  [11] memoise_2.0.1               Rsamtools_2.28.0           
##  [13] RCurl_1.98-1.18             base64enc_0.1-6            
##  [15] htmltools_0.5.9             S4Arrays_1.12.0            
##  [17] progress_1.2.3              curl_7.1.0                 
##  [19] SparseArray_1.12.1          Formula_1.2-5              
##  [21] sass_0.4.10                 bslib_0.10.0               
##  [23] htmlwidgets_1.6.4           Gviz_1.56.0                
##  [25] httr2_1.2.2                 cachem_1.1.0               
##  [27] buildtools_1.0.0            GenomicAlignments_1.48.0   
##  [29] lifecycle_1.0.5             pkgconfig_2.0.3            
##  [31] Matrix_1.7-5                R6_2.6.1                   
##  [33] fastmap_1.2.0               MatrixGenerics_1.24.0      
##  [35] digest_0.6.39               colorspace_2.1-2           
##  [37] AnnotationDbi_1.74.0        S4Vectors_0.50.0           
##  [39] Hmisc_5.2-5                 GenomicRanges_1.64.0       
##  [41] RSQLite_2.4.6               filelock_1.0.3             
##  [43] randomForest_4.7-1.2        httr_1.4.8                 
##  [45] abind_1.4-8                 compiler_4.6.0             
##  [47] Rbowtie_1.52.0              bit64_4.8.0                
##  [49] htmlTable_2.5.0             S7_0.2.2                   
##  [51] backports_1.5.1             BiocParallel_1.46.0        
##  [53] DBI_1.3.0                   biomaRt_2.68.0             
##  [55] rappdirs_0.3.4              DelayedArray_0.38.1        
##  [57] rjson_0.2.23                tools_4.6.0                
##  [59] foreign_0.8-91              nnet_7.3-20                
##  [61] glue_1.8.1                  restfulr_0.0.16            
##  [63] grid_4.6.0                  checkmate_2.3.4            
##  [65] cluster_2.1.8.2             gtable_0.3.6               
##  [67] BSgenome_1.80.0             tzdb_0.5.0                 
##  [69] ensembldb_2.36.0            data.table_1.18.2.1        
##  [71] hms_1.1.4                   XVector_0.52.0             
##  [73] BiocVersion_3.23.1          pillar_1.11.1              
##  [75] stringr_1.6.0               dplyr_1.2.1                
##  [77] lattice_0.22-9              deldir_2.0-4               
##  [79] rtracklayer_1.72.0          bit_4.6.0                  
##  [81] biovizBase_1.60.0           tidyselect_1.2.1           
##  [83] maketools_1.3.2             Biostrings_2.80.0          
##  [85] knitr_1.51                  gridExtra_2.3              
##  [87] ProtGenerics_1.44.0         IRanges_2.46.0             
##  [89] Seqinfo_1.2.0               SummarizedExperiment_1.42.0
##  [91] stats4_4.6.0                xfun_0.57                  
##  [93] Biobase_2.72.0              matrixStats_1.5.0          
##  [95] stringi_1.8.7               UCSC.utils_1.8.0           
##  [97] lazyeval_0.2.3              yaml_2.3.12                
##  [99] evaluate_1.0.5              codetools_0.2-20           
## [101] cigarillo_1.2.0             interp_1.1-6               
## [103] tibble_3.3.1                BiocManager_1.30.27        
## [105] cli_3.6.6                   rpart_4.1.27               
## [107] reticulate_1.46.0           jquerylib_0.1.4            
## [109] dichromat_2.0-0.1           Rcpp_1.1.1-1.1             
## [111] GenomeInfoDb_1.48.0         png_0.1-9                  
## [113] XML_3.99-0.23               parallel_4.6.0             
## [115] ggplot2_4.0.3               readr_2.2.0                
## [117] blob_1.3.0                  prettyunits_1.2.0          
## [119] jpeg_0.1-11                 latticeExtra_0.6-31        
## [121] AnnotationFilter_1.36.0     bitops_1.0-9               
## [123] txdbmaker_1.8.0             VariantAnnotation_1.58.0   
## [125] scales_1.4.0                crayon_1.5.3               
## [127] rlang_1.2.0                 KEGGREST_1.52.0