## ----eval=FALSE--------------------------------------------------------------- # tiff <- readTIFF() # data <- readBeadLevelTextFile() ## ----eval=FALSE--------------------------------------------------------------- # bg <- illuminaBackground(tiff, data[,3:4]) # tiffSharp <- illuminaSharpen(tiff) # fg <- illuminaForeground(tiffSharp, data[,3:4]) # finalIntensity <- fg - bg ## ----eval=FALSE--------------------------------------------------------------- # BLData <- new(Class = "beadLevelData") # BLData <- insertBeadData(BLData, array = 1, what = "Grn", data = finalIntensity) ## ----eval=FALSE--------------------------------------------------------------- # BLData <- readIllumina() # BLData <- insertBeadData(BLData, array = 1, what = "Grn", data = finalIntensity) # BLData <- insertBeadData(BLData, array = 1, what = "GrnLog", data = log2(finalIntensity)) # ## ----eval=FALSE--------------------------------------------------------------- # bg <- medianBackground(tiff, data[,3:4]);