Report breakdown by barcode
ampliCan
01 November 2022
1 Description
Read distribution plot - plot shows number of reads assigned during read grouping
Filtered Reads - plot shows percentage of assigned reads that have been recognized as PRIMER DIMERS or filtered based on low alignment score
Edit rates - plot gives overview of percentage of reads (not filtered as PRIMER DIMER) that have edits
Frameshift - plot shows what percentage of reads that have frameshift
Read heterogeneity plot - shows what is the share of each of the unique reads in total count of all reads. The more yellow each row, the less heterogeneity in the reads, more black means reads don’t repeat often and are unique
Top unassigned reads - take a look at the alignment of most abundant forward and reverse complemented reverse reads for each barcode, if you find that there is many unassigned reads you can ivestigate here.
2 Barcode Summary
2.1 Groups IDs
| group | IDs |
|---|---|
| barcode_1 | ID_1, ID_2 |
| barcode_2 | ID_3, ID_4, ID_5 |
2.2 Read distribution
2.3 Filtered Reads
2.4 Edit rates
2.5 Frameshift
2.6 Heterogeneity of reads
3 Top unassigned reads
3.1 barcode_1
| Forward | Reverse | Counts | Frequency |
|---|---|---|---|
| P1 | S1 | 1 | 0.0588235 |
| P2 | S2 | 1 | 0.0588235 |
| P3 | S3 | 1 | 0.0588235 |
| P4 | S4 | 1 | 0.0588235 |
P1 1 AAATACTGTCTTGTGACCAAACCTTCTTAAGGTGCTGTTTTGATGATAAA 50
|| ||| | | | ||| | || ||| |||
S1 1 AAG--CTGAC----GGCTAAA---TGAAAAATATCTG----------AAA 31
P1 51 CTTTATTGTGCTTTTGTAGTTGTGCCCCTTGTGTT--GGCAGAG-----G 93
| | || || || |||| | | || ||||||| |
S1 32 CAT-------CTGTTCCAG--GTGCTGCGTATGCCAGGGCAGAGAAGAAG 72
P1 94 GTCAGC--AGACCAGT--AAGTCTTCTCAATTTCTTTTATTT----ATGT 135
||||| || || | | ||| || | ||| || |
S1 73 GTCAGGGAAGGTCACTGGAGGTCACTGGGATACCCTTTCTTCCCACACCA 122
P1 136 AT-----ATGTAGT-------GATAAA-A 151
|| | | ||| ||| | |
S1 123 ATGGGGAAAGGAGTCCTGCCAGATGACCA 151
P2 1 AAATACTGTCTTGTGACCAAACCTTCTTAAGGTGCTG---TTTTGATGAT 47
| || | || | | | | ||| | || || ||
S2 1 --------TTTTATCACTACATATACATAAATAAAAGAAATTGAGAAGAC 42
P2 48 NNNCTTTATTGTGCTTTTGTAGTTGTGCCCCTTGTGTTGGCAGAGGGTCA 97
||| || || || | |||| |||| | ||
S2 43 -----TTACTGGTCTGCTGACCCTCTGCCAACACAAGGGGCACAACTACA 87
P2 98 GCAGACCAGTAAG-----TCTTCTCAATTTCTTTTATTTATGTATATGTA 142
|| || ||| || || || | | || | | | |
S2 88 AAAGCACAATAAAGNNNATCATCAAAACAGCACCT---TAAGAAGGTTTG 134
P2 143 GTGATAAAA-------- 151
|| | || |
S2 135 GTCACAAGACAGTATTT 151
P3 1 AAGCTGACGGCTAAATNNNAAATATCTGAAACATCTGTTCCAGGTGCTGC 50
|||||||||||||||| |||||||||||||||||||||||||||||||
S3 1 AAGCTGACGGCTAAATGAAAAATATCTGAAACATCTGTTCCAGGTGCTGC 50
P3 51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG 100
||||||||||||||||||||||||||||||||||||||||||||||||||
S3 51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG 100
P3 101 GATACCCTTTCTTCCCACACCAATGGGGAAAGGAGTCCTGCCAGATGACC 150
||||||||||||||||||||||||||||||||||||||||||||||||||
S3 101 GATACCCTTTCTTCCCACACCAATGGGGAAAGGAGTCCTGCCAGATGACC 150
P3 151 A 151
|
S3 151 A 151
P4 1 AAGCTNGCGGCTAAATGAAAAATATCTGAAACATCTGTTCCAGGTGCTGC 50
|| |||||||||||||||||||||||||||||||||||||||||||
S4 1 NNNCTGACGGCTAAATGAAAAATATCTGAAACATCTGTTCCAGGTGCTGC 50
P4 51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG 100
||||||||||||||||||||||||||||||||||||||||||||||||||
S4 51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG 100
P4 101 GATACCCTTTCTTCCCACACCANNNGGGAAAGGAGTCCTGCCAGATGACC 150
||||||| |||||||||||| |||||||||||||| |||||||
S4 101 GATACCCNNNCTTCCCACACCAATGGGGAAAGGAGTCCTNNNNGATGACC 150
P4 151 A 151
|
S4 151 A 151