############################################################################## ############################################################################## ### ### Running command: ### ### /home/biocbuild/bbs-3.17-bioc/R/bin/R CMD check --install=check:SpliceWiz.install-out.txt --library=/home/biocbuild/bbs-3.17-bioc/R/site-library --timings SpliceWiz_1.1.7.tar.gz ### ############################################################################## ############################################################################## * using log directory ‘/home/biocbuild/bbs-3.17-bioc/meat/SpliceWiz.Rcheck’ * using R version 4.3.0 alpha (2023-04-03 r84154) * using platform: x86_64-pc-linux-gnu (64-bit) * R was compiled by gcc (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0 GNU Fortran (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0 * running under: Ubuntu 22.04.2 LTS * using session charset: UTF-8 * checking for file ‘SpliceWiz/DESCRIPTION’ ... OK * this is package ‘SpliceWiz’ version ‘1.1.7’ * package encoding: UTF-8 * checking package namespace information ... OK * checking package dependencies ... OK * checking if this is a source package ... OK * checking if there is a namespace ... OK * checking for hidden files and directories ... OK * checking for portable file names ... OK * checking for sufficient/correct file permissions ... OK * checking whether package ‘SpliceWiz’ can be installed ... OK * used C++ compiler: ‘g++ (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0’ * checking C++ specification ... NOTE Specified C++11: please drop specification unless essential * checking installed package size ... NOTE installed size is 8.1Mb sub-directories of 1Mb or more: R 2.0Mb libs 4.9Mb * checking package directory ... OK * checking ‘build’ directory ... OK * checking DESCRIPTION meta-information ... OK * checking top-level files ... OK * checking for left-over files ... OK * checking index information ... OK * checking package subdirectories ... OK * checking R files for non-ASCII characters ... OK * checking R files for syntax errors ... OK * checking whether the package can be loaded ... OK * checking whether the package can be loaded with stated dependencies ... OK * checking whether the package can be unloaded cleanly ... OK * checking whether the namespace can be loaded with stated dependencies ... OK * checking whether the namespace can be unloaded cleanly ... OK * checking loading without being on the library search path ... OK * checking startup messages can be suppressed ... OK * checking dependencies in R code ... OK * checking S3 generic/method consistency ... OK * checking replacement functions ... OK * checking foreign function calls ... OK * checking R code for possible problems ... OK * checking Rd files ... OK * checking Rd metadata ... OK * checking Rd cross-references ... OK * checking for missing documentation entries ... OK * checking for code/documentation mismatches ... OK * checking Rd \usage sections ... OK * checking Rd contents ... OK * checking for unstated dependencies in examples ... OK * checking line endings in shell scripts ... OK * checking line endings in C/C++/Fortran sources/headers ... OK * checking line endings in Makefiles ... OK * checking compilation flags in Makevars ... OK * checking for GNU extensions in Makefiles ... OK * checking for portable use of $(BLAS_LIBS) and $(LAPACK_LIBS) ... OK * checking use of PKG_*FLAGS in Makefiles ... OK * checking compiled code ... NOTE Note: information on .o files is not available * checking files in ‘vignettes’ ... OK * checking examples ... ERROR Running examples in ‘SpliceWiz-Ex.R’ failed The error most likely occurred in: > base::assign(".ptime", proc.time(), pos = "CheckExEnv") > ### Name: getAvailableGO > ### Title: Builds reference files used by SpliceWiz > ### Aliases: getAvailableGO Build-Reference-methods getResources buildRef > ### buildFullRef getNonPolyARef > > ### ** Examples > > # Quick runnable example: generate a reference using SpliceWiz's example genome > > example_ref <- file.path(tempdir(), "Reference") > getResources( + reference_path = example_ref, + fasta = chrZ_genome(), + gtf = chrZ_gtf() + ) Apr 11 23:56:35 Converting FASTA to local TwoBitFile...done Apr 11 23:56:36 Connecting to genome TwoBitFile...done Apr 11 23:56:36 Making local copy of GTF file...done > buildRef( + reference_path = example_ref + ) Apr 11 23:56:37 Reference generated without non-polyA reference Apr 11 23:56:37 Reference generated without Mappability reference Apr 11 23:56:37 Reference generated without Blacklist exclusion Apr 11 23:56:37 Connecting to genome TwoBitFile...done Apr 11 23:56:37 Reading source GTF file...done Apr 11 23:56:37 Processing gtf file... ...genes ...transcripts ...CDS ...exons done Apr 11 23:56:38 Gene ontology not prepared for this reference Apr 11 23:56:40 Processing introns... ...data ...basic annotations ...splice motifs ...other info ...defining flanking exon clusters done Apr 11 23:56:45 Generating processBAM reference ...prepping data ...determining measurable introns (directional) ...determining measurable introns (non-directional) ...writing ref-cover.bed ...writing ref-ROI.bed ...writing ref-read-continues.ref ...writing ref-sj.ref ...writing ref-tj.ref processBAM reference generated Apr 11 23:56:53 Predicting NMD transcripts from genome sequence ...exonic transcripts ...retained introns | | | 0% | |======================================================================| 100% done Apr 11 23:56:55 Annotating Splice Events Annotating Mutually-Exclusive-Exon Splice Events...done Annotating Skipped-Exon Splice Events...done Annotating Alternate 5' / 3' Splice Site Splice Events...done Annotating Alternate First / Last Exon Splice Events...done Annotating known retained introns...done Apr 11 23:56:56 Splice Annotations Filtered Apr 11 23:56:57 Translating Alternate Splice Peptides...done Apr 11 23:56:58 Splice Annotations finished Reference build finished > > # NB: the above is equivalent to: > > example_ref <- file.path(tempdir(), "Reference") > buildRef( + reference_path = example_ref, + fasta = chrZ_genome(), + gtf = chrZ_gtf() + ) Apr 11 23:56:59 SpliceWiz reference already exists in given directory NULL > > # Get the path to the Non-PolyA BED file for hg19 > > getNonPolyARef("hg19") [1] "/home/biocbuild/bbs-3.17-bioc/R/site-library/SpliceWiz/extra-input-files/Human_hg19_nonPolyA_ROI.bed" > > # View available species for AnnotationHub's Ensembl/orgDB-based GO resources > > availSpecies <- getAvailableGO() > > # Build example reference with `Homo sapiens` Ens/orgDB gene ontology > > ont_ref <- file.path(tempdir(), "Reference_withGO") > buildRef( + reference_path = ont_ref, + fasta = chrZ_genome(), + gtf = chrZ_gtf(), + ontologySpecies = "Homo sapiens" + ) Apr 11 23:57:02 Reference generated without non-polyA reference Apr 11 23:57:02 Reference generated without Mappability reference Apr 11 23:57:02 Reference generated without Blacklist exclusion Apr 11 23:57:02 Converting FASTA to local TwoBitFile...done Apr 11 23:57:04 Connecting to genome TwoBitFile...done Apr 11 23:57:04 Making local copy of GTF file...done Apr 11 23:57:04 Reading source GTF file...done Apr 11 23:57:04 Processing gtf file... ...genes ...transcripts ...CDS ...exons done Apr 11 23:57:08 Retrieving gene GO-term pairings Error in if (any(bad)) stop("invalid subscripts: ", paste(sQuote(S4Vectors:::selectSome(bad)), : missing value where TRUE/FALSE needed Calls: buildRef ... .fetch_orgDB -> subset -> subset.default -> [ -> [ -> .db_uid<- Execution halted * checking for unstated dependencies in ‘tests’ ... OK * checking tests ... Running ‘testthat.R’ OK * checking for unstated dependencies in vignettes ... OK * checking package vignettes in ‘inst/doc’ ... OK * checking running R code from vignettes ... ‘SW_Cookbook.Rmd’ using ‘UTF-8’... OK ‘SW_QuickStart.Rmd’ using ‘UTF-8’... OK NONE * checking re-building of vignette outputs ... OK * checking PDF version of manual ... OK * DONE Status: 1 ERROR, 3 NOTEs See ‘/home/biocbuild/bbs-3.17-bioc/meat/SpliceWiz.Rcheck/00check.log’ for details.