MaxContrastProjection

Perform a maximum contrast projection of 3D images along the z-dimension into 2D

Bioconductor version: Release (3.5)

A problem when recording 3D fluorescent microscopy images is how to properly present these results in 2D. Maximum intensity projections are a popular method to determine the focal plane of each pixel in the image. The problem with this approach, however, is that out-of-focus elements will still be visible, making edges and fine structures difficult to detect. This package aims to resolve this problem by using the contrast around a given pixel to determine the focal plane, allowing for a much cleaner structure detection than would be otherwise possible. For convenience, this package also contains functions to perform various other types of projections, including a maximum intensity projection.

Author: Jan Sauer, Bernd Fischer

Maintainer: Jan Sauer <jan.sauer at dkfz-heidelberg.de>

Installation

To install this package, start R and enter:

## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("MaxContrastProjection")

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("MaxContrastProjection")

Details

Package Archives

Follow Installation instructions to use this package in your R session.