Package: BSgenome.Drerio.UCSC.danRer10 Title: Full genome sequences for Danio rerio (UCSC version danRer10) Description: Full genome sequences for Danio rerio (Zebrafish) as provided by UCSC (danRer10, Sep. 2014) and stored in Biostrings objects. Version: 1.4.2 Suggests: GenomicFeatures organism: Danio rerio common_name: Zebrafish provider: UCSC provider_version: danRer10 release_date: Sep. 2014 release_name: Genome Reference Consortium GRCz10 source_url: http://hgdownload.soe.ucsc.edu/goldenPath/danRer10/bigZips/ organism_biocview: Danio_rerio BSgenomeObjname: Drerio circ_seqs: "chrM" SrcDataFiles: danRer10.2bit from http://hgdownload.soe.ucsc.edu/goldenPath/danRer10/bigZips/ PkgExamples: genome$chr1 # same as genome[["chr1"]] . ## --------------------------------------------------------------------- ## Extract the upstream sequences ## --------------------------------------------------------------------- ## The upstream sequences located in ## http://hgdownload.cse.ucsc.edu/goldenPath/danRer10/bigZips/ ## are based on RefSeq genes (RefSeq Genes track in the Genome Browser). ## These can easily be extracted from the full genome sequences with: . library(GenomicFeatures) refGene_txdb <- suppressWarnings(makeTxDbFromUCSC("danRer10", "refGene")) refGene_up1000seqs <- extractUpstreamSeqs(genome, refGene_txdb) . ## Note that you can make a TxDb object from various annotation ## resources. See the makeTxDbFromUCSC(), makeTxDbFromBiomart(), and ## makeTxDbFromGFF() functions in the GenomicFeatures package for more ## information. ## IMPORTANT: Make sure you use a TxDb package (or TxDb object) that ## contains a gene model based on danRer10 or on a compatible genome ## (i.e. a genome with sequences identical to the sequences in ## danRer10). See ?extractUpstreamSeqs in the GenomicFeatures package ## for more information. seqs_srcdir: /fh/fast/morgan_m/BioC/BSgenomeForge/srcdata/BSgenome.Drerio.UCSC.danRer10/seqs seqfile_name: danRer10.sorted.2bit