scp 1.2.0
scpdata packagescpdata disseminates mass spectrometry (MS)-based single-cell
proteomics (SCP) data sets formatted using the scp data structure.
The data structure is described in the
scp vignette.
In this vignette, we describe how to access the SCP data sets. To
start, we load the scpdata package.
library("scpdata")ExperimentHubThe data is stored using the
ExperimentHub
infrastructure. We first create a connection with ExperimentHub.
eh <- ExperimentHub()You can list all data sets available in scpdata using the query
function.
query(eh, "scpdata")
#> ExperimentHub with 12 records
#> # snapshotDate(): 2021-05-05
#> # $dataprovider: PRIDE, MassIVE, SlavovLab website
#> # $species: Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus
#> # $rdataclass: QFeatures
#> # additional mcols(): taxonomyid, genome, description,
#> # coordinate_1_based, maintainer, rdatadateadded, preparerclass, tags,
#> # rdatapath, sourceurl, sourcetype
#> # retrieve records with, e.g., 'object[["EH3899"]]'
#>
#> title
#> EH3899 | specht2019v2
#> EH3900 | specht2019v3
#> EH3901 | dou2019_lysates
#> EH3902 | dou2019_mouse
#> EH3903 | dou2019_boosting
#> ... ...
#> EH3906 | zhu2018NC_lysates
#> EH3907 | zhu2018NC_islets
#> EH3908 | cong2020AC
#> EH3909 | zhu2019EL
#> EH6011 | liang2020_helaAnother way to get information about the available data sets is to
call scpdata(). This will retrieve all the available metadata. For
example, we can retrieve the data set titles along with the
description to make an informed choice about which data set to choose.
info <- scpdata()
#> snapshotDate(): 2021-05-05
knitr::kable(info[, c("title", "description")])| title | description | |
|---|---|---|
| EH3899 | specht2019v2 | SCP expression data for monocytes (U-937) and macrophages at PSM, peptide and protein level |
| EH3900 | specht2019v3 | SCP expression data for more monocytes (U-937) and macrophages at PSM, peptide and protein level |
| EH3901 | dou2019_lysates | SCP expression data for Hela digests (0.2 or 10 ng) at PSM and protein level |
| EH3902 | dou2019_mouse | SCP expression data for C10, SVEC or Raw cells at PSM and protein level |
| EH3903 | dou2019_boosting | SCP expression data for C10, SVEC or Raw cells and 3 boosters (0, 5 or 50 ng) at PSM and protein level |
| EH3904 | zhu2018MCP | Near SCP expression data for micro-dissection rat brain samples (50, 100, or 200 ยตm width) at PSM level |
| EH3905 | zhu2018NC_hela | Near SCP expression data for HeLa samples (aproximately 12, 40, or 140 cells) at PSM level |
| EH3906 | zhu2018NC_lysates | Near SCP expression data for HeLa lysates (10, 40 and 140 cell equivalent) at PSM level |
| EH3907 | zhu2018NC_islets | Near SCP expression data for micro-dissected human pancreas samples (control patients or type 1 diabetes) at PSM level |
| EH3908 | cong2020AC | SCP expression data for Hela cells at PSM, peptide and protein level |
| EH3909 | zhu2019EL | SCP expression data for chicken utricle samples (1, 3, 5 or 20 cells) at PSM, peptide and protein level |
| EH6011 | liang2020_hela | Expression data for HeLa cells (0, 1, 10, 150, 500 cells) at PSM, peptide and protein level |
To get one of the data sets (e.g. dou2019_lysates) you can either
retrieve it using the ExperimentHub query function
scp <- eh[["EH3901"]]
#> see ?scpdata and browseVignettes('scpdata') for documentation
#> loading from cache
scp
#> An instance of class QFeatures containing 3 assays:
#> [1] Hela_run_1: SingleCellExperiment with 35224 rows and 10 columns
#> [2] Hela_run_2: SingleCellExperiment with 35080 rows and 10 columns
#> [3] proteins: SingleCellExperiment with 1641 rows and 20 columnsor you can the use the built-in functions from scpdata
scp <- dou2019_lysates()
#> see ?scpdata and browseVignettes('scpdata') for documentation
#> loading from cache
scp
#> An instance of class QFeatures containing 3 assays:
#> [1] Hela_run_1: SingleCellExperiment with 35224 rows and 10 columns
#> [2] Hela_run_2: SingleCellExperiment with 35080 rows and 10 columns
#> [3] proteins: SingleCellExperiment with 1641 rows and 20 columnsEach data set has been extensively documented in a separate man page
(e.g. ?dou2019_lysates). You can find information about the data
content, the acquisition protocol, the data collection procedure as
well as the data sources and reference.
For more information about manipulating the data sets, check the
scp
package. The scp
vignette
will guide you through a typical SCP data processing workflow. Once
your data is loaded from scpdata you can skip section 2
Read in SCP data of the scp vignette.
R version 4.1.0 (2021-05-18)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 20.04.2 LTS
Matrix products: default
BLAS: /home/biocbuild/bbs-3.13-bioc/R/lib/libRblas.so
LAPACK: /home/biocbuild/bbs-3.13-bioc/R/lib/libRlapack.so
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=C
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] parallel stats4 stats graphics grDevices utils datasets
[8] methods base
other attached packages:
[1] scpdata_1.0.0 ExperimentHub_2.0.0
[3] AnnotationHub_3.0.0 BiocFileCache_2.0.0
[5] dbplyr_2.1.1 QFeatures_1.2.0
[7] MultiAssayExperiment_1.18.0 SummarizedExperiment_1.22.0
[9] Biobase_2.52.0 GenomicRanges_1.44.0
[11] GenomeInfoDb_1.28.0 IRanges_2.26.0
[13] S4Vectors_0.30.0 BiocGenerics_0.38.0
[15] MatrixGenerics_1.4.0 matrixStats_0.58.0
[17] BiocStyle_2.20.0
loaded via a namespace (and not attached):
[1] ProtGenerics_1.24.0 bitops_1.0-7
[3] bit64_4.0.5 filelock_1.0.2
[5] httr_1.4.2 tools_4.1.0
[7] bslib_0.2.5.1 utf8_1.2.1
[9] R6_2.5.0 DBI_1.1.1
[11] lazyeval_0.2.2 withr_2.4.2
[13] tidyselect_1.1.1 bit_4.0.4
[15] curl_4.3.1 compiler_4.1.0
[17] DelayedArray_0.18.0 bookdown_0.22
[19] sass_0.4.0 rappdirs_0.3.3
[21] stringr_1.4.0 digest_0.6.27
[23] rmarkdown_2.8 XVector_0.32.0
[25] pkgconfig_2.0.3 htmltools_0.5.1.1
[27] highr_0.9 fastmap_1.1.0
[29] rlang_0.4.11 RSQLite_2.2.7
[31] shiny_1.6.0 jquerylib_0.1.4
[33] generics_0.1.0 jsonlite_1.7.2
[35] dplyr_1.0.6 RCurl_1.98-1.3
[37] magrittr_2.0.1 GenomeInfoDbData_1.2.6
[39] Matrix_1.3-3 Rcpp_1.0.6
[41] fansi_0.4.2 MsCoreUtils_1.4.0
[43] lifecycle_1.0.0 stringi_1.6.2
[45] yaml_2.2.1 MASS_7.3-54
[47] zlibbioc_1.38.0 grid_4.1.0
[49] blob_1.2.1 promises_1.2.0.1
[51] crayon_1.4.1 lattice_0.20-44
[53] Biostrings_2.60.0 KEGGREST_1.32.0
[55] knitr_1.33 pillar_1.6.1
[57] glue_1.4.2 BiocVersion_3.13.1
[59] evaluate_0.14 BiocManager_1.30.15
[61] vctrs_0.3.8 png_0.1-7
[63] httpuv_1.6.1 purrr_0.3.4
[65] clue_0.3-59 assertthat_0.2.1
[67] cachem_1.0.5 xfun_0.23
[69] mime_0.10 xtable_1.8-4
[71] AnnotationFilter_1.16.0 later_1.2.0
[73] SingleCellExperiment_1.14.0 tibble_3.1.2
[75] AnnotationDbi_1.54.0 memoise_2.0.0
[77] cluster_2.1.2 ellipsis_0.3.2
[79] interactiveDisplayBase_1.30.0