Understanding the barcode
A TCGA barcode is composed of a collection of identifiers. Each specifically identifies a TCGA data element. Refer to the following figure for an illustration of how metadata identifiers comprise a barcode. An aliquot barcode contains the highest number of identifiers.
Example:
- Aliquot barcode: TCGA-G4-6317-02A-11D-2064-05
- Participant: TCGA-G4-6317
- Sample: TCGA-G4-6317-02
For more information check [GDC TCGA barcodes](https://docs.gdc.cancer.gov/Encyclopedia/pages/TCGA_Barcode/)
# Searching arguments
You can easily search GDC data using the `GDCquery` function.
Using a summary of filters as used in the TCGA portal, the function works
with the following arguments:
| ?project | A list of valid project (see table below)] | |
|----------------------- |------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |------------------------------------- |
| data.category | A valid project (see list with TCGAbiolinks:::getProjectSummary(project)) | |
| data.type | A data type to filter the files to download | |
| workflow.type | GDC workflow type | |
| access | Filter by access type. Possible values: controlled, open | |
| platform | Example: | |
| | CGH- 1x1M_G4447A | IlluminaGA_RNASeqV2 |
| | AgilentG4502A_07 | IlluminaGA_mRNA_DGE |
| | Human1MDuo | HumanMethylation450 |
| | HG-CGH-415K_G4124A | IlluminaGA_miRNASeq |
| | HumanHap550 | IlluminaHiSeq_miRNASeq |
| | ABI | H-miRNA_8x15K |
| | HG-CGH-244A | SOLiD_DNASeq |
| | IlluminaDNAMethylation_OMA003_CPI | IlluminaGA_DNASeq_automated |
| | IlluminaDNAMethylation_OMA002_CPI | HG-U133_Plus_2 |
| | HuEx- 1_0-st-v2 | Mixed_DNASeq |
| | H-miRNA_8x15Kv2 | IlluminaGA_DNASeq_curated |
| | MDA_RPPA_Core | IlluminaHiSeq_TotalRNASeqV2 |
| | HT_HG-U133A | IlluminaHiSeq_DNASeq_automated |
| | diagnostic_images | microsat_i |
| | IlluminaHiSeq_RNASeq | SOLiD_DNASeq_curated |
| | IlluminaHiSeq_DNASeqC | Mixed_DNASeq_curated |
| | IlluminaGA_RNASeq | IlluminaGA_DNASeq_Cont_automated |
| | IlluminaGA_DNASeq | IlluminaHiSeq_WGBS |
| | pathology_reports | IlluminaHiSeq_DNASeq_Cont_automated |
| | Genome_Wide_SNP_6 | bio |
| | tissue_images | Mixed_DNASeq_automated |
| | HumanMethylation27 | Mixed_DNASeq_Cont_curated |
| | IlluminaHiSeq_RNASeqV2 | Mixed_DNASeq_Cont |
| file.type | To be used in the legacy database for some platforms, to define which file types to be used. | |
| barcode | A list of barcodes to filter the files to download | |
| experimental.strategy | Filter to experimental strategy. Harmonized: WXS, RNA-Seq, miRNA-Seq, Genotyping Array. | |
| sample.type | A sample type to filter the files to download | |
## project options
The options for the field `project` are below:
```{r, eval = TRUE, echo = FALSE}
datatable(
TCGAbiolinks:::getGDCprojects(),
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 10),
rownames = FALSE,
caption = "List of projects"
)
```
## sample.type options
The options for the field `sample.type` are below:
```{r, eval = TRUE, echo = FALSE}
datatable(
TCGAbiolinks:::getBarcodeDefinition(),
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 10),
rownames = FALSE,
caption = "List sample types"
)
```
The other fields (data.category, data.type, workflow.type, platform, file.type) can be found below.
Please, note that these tables are still incomplete.
## Harmonized data options
```{r, echo=FALSE}
datatable(
readr::read_csv("https://docs.google.com/spreadsheets/d/1f98kFdj9mxVDc1dv4xTZdx8iWgUiDYO-qiFJINvmTZs/export?format=csv&gid=2046985454",col_types = readr::cols()),
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 40),
rownames = FALSE
)
```
# Harmonized database examples
## DNA methylation data: Recurrent tumor samples
In this example we will access the harmonized database
and search for all DNA methylation data for recurrent glioblastoma multiform (GBM)
and low grade gliomas (LGG) samples.
```{r message=FALSE, warning=FALSE}
query <- GDCquery(
project = c("TCGA-GBM", "TCGA-LGG"),
data.category = "DNA Methylation",
platform = c("Illumina Human Methylation 450"),
sample.type = "Recurrent Tumor"
)
datatable(
getResults(query),
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 5),
rownames = FALSE
)
```
## Samples with DNA methylation and gene expression data
In this example we will access the harmonized database
and search for all patients with DNA methylation (platform HumanMethylation450k) and gene expression data
for Colon Adenocarcinoma tumor (TCGA-COAD).
```{r message=FALSE, warning = FALSE, eval = FALSE}
query_met <- GDCquery(
project = "TCGA-COAD",
data.category = "DNA Methylation",
platform = c("Illumina Human Methylation 450")
)
query_exp <- GDCquery(
project = "TCGA-COAD",
data.category = "Transcriptome Profiling",
data.type = "Gene Expression Quantification",
workflow.type = "STAR - Counts"
)
# Get all patients that have DNA methylation and gene expression.
common.patients <- intersect(
substr(getResults(query_met, cols = "cases"), 1, 12),
substr(getResults(query_exp, cols = "cases"), 1, 12)
)
# Only seelct the first 5 patients
query_met <- GDCquery(
project = "TCGA-COAD",
data.category = "DNA Methylation",
platform = c("Illumina Human Methylation 450"),
barcode = common.patients[1:5]
)
query_exp <- GDCquery(
project = "TCGA-COAD",
data.category = "Transcriptome Profiling",
data.type = "Gene Expression Quantification",
workflow.type = "STAR - Counts",
barcode = common.patients[1:5]
)
```
```{r results_matched, message=FALSE, warning=FALSE, eval = FALSE}
datatable(
getResults(query_met, cols = c("data_type","cases")),
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 5),
rownames = FALSE
)
datatable(
getResults(query_exp, cols = c("data_type","cases")),
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 5),
rownames = FALSE
)
```
## Raw Sequencing Data: Finding the match between file names and barcode for Controlled data.
This example shows how the user can search for breast cancer Raw Sequencing Data ("Controlled")
and verify the name of the files and the barcodes associated with it.
```{r message=FALSE, warning=FALSE}
query <- GDCquery(
project = "TCGA-ACC",
data.category = "Sequencing Reads",
data.type = "Aligned Reads",
data.format = "bam",
workflow.type = "STAR 2-Pass Transcriptome"
)
# Only first 10 to make render faster
datatable(
getResults(query, rows = 1:10,cols = c("file_name","cases")),
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 5),
rownames = FALSE
)
query <- GDCquery(
project = "TCGA-ACC",
data.category = "Sequencing Reads",
data.type = "Aligned Reads",
data.format = "bam",
workflow.type = "STAR 2-Pass Genome"
)
# Only first 10 to make render faster
datatable(
getResults(query, rows = 1:10,cols = c("file_name","cases")),
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 5),
rownames = FALSE
)
query <- GDCquery(
project = "TCGA-ACC",
data.category = "Sequencing Reads",
data.type = "Aligned Reads",
data.format = "bam",
workflow.type = "STAR 2-Pass Chimeric"
)
# Only first 10 to make render faster
datatable(
getResults(query, rows = 1:10,cols = c("file_name","cases")),
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 5),
rownames = FALSE
)
query <- GDCquery(
project = "TCGA-ACC",
data.category = "Sequencing Reads",
data.type = "Aligned Reads",
data.format = "bam",
workflow.type = "BWA-aln"
)
# Only first 10 to make render faster
datatable(
getResults(query, rows = 1:10,cols = c("file_name","cases")),
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 5),
rownames = FALSE
)
query <- GDCquery(
project = "TCGA-ACC",
data.category = "Sequencing Reads",
data.type = "Aligned Reads",
data.format = "bam",
workflow.type = "BWA with Mark Duplicates and BQSR"
)
# Only first 10 to make render faster
datatable(
getResults(query, rows = 1:10,cols = c("file_name","cases")),
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 5),
rownames = FALSE
)
```
# Get Manifest file
If you want to get the manifest file from the query object you can use the function *getManifest*. If you
set save to `TRUE` a txt file that can be used with GDC-client Data transfer tool (DTT) or with its GUI version [ddt-ui](https://github.com/NCI-GDC/dtt-ui) will be created.
```{r message=FALSE, warning=FALSE}
getManifest(query,save = FALSE)
```
# ATAC-seq data
For the moment, ATAC-seq data is available at the [GDC publication page](https://gdc.cancer.gov/about-data/publications/ATACseq-AWG).
Also, for more details, you can check an ATAC-seq workshop at http://rpubs.com/tiagochst/atac_seq_workshop
The list of file available is below:
```{r message=FALSE, warning=FALSE}
datatable(
getResults(TCGAbiolinks:::GDCquery_ATAC_seq())[,c("file_name","file_size")],
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 5),
rownames = FALSE
)
```
You can use the function `GDCquery_ATAC_seq` filter the manifest table and use `GDCdownload` to save the data locally.
```{r message=FALSE, warning=FALSE,eval = FALSE}
query <- TCGAbiolinks:::GDCquery_ATAC_seq(file.type = "rds")
GDCdownload(query, method = "client")
query <- TCGAbiolinks:::GDCquery_ATAC_seq(file.type = "bigWigs")
GDCdownload(query, method = "client")
```
# Summary of available files per patient
Retrieve the numner of files under each data_category + data_type + experimental_strategy + platform.
Almost like https://portal.gdc.cancer.gov/exploration
```{r message=FALSE, warning=FALSE,eval = TRUE}
tab <- getSampleFilesSummary(project = "TCGA-ACC")
datatable(
head(tab),
filter = 'top',
options = list(scrollX = TRUE, keys = TRUE, pageLength = 5),
rownames = FALSE
)
```