--- title: "Macarron User Manual" author: - name: Amrisha Bhosle email: abhosle@broadinstitute.org - name: Ludwig Geistlinger email: ludwig_geistlinger@hms.harvard.edu - name: Sagun Maharjan email: sagunmaharjann@gmail.com output: BiocStyle::html_document: toc: true toc_float: true vignette: > %\VignetteIndexEntry{Macarron} %\VignetteEncoding{UTF-8} %\VignetteEngine{knitr::rmarkdown} --- # Abstract [Macarron](https://huttenhower.sph.harvard.edu/macarron/) is a workflow to systematically annotate and prioritize potentially bioactive (and often unannotated) small molecules in microbial community metabolomic datasets. Macarron prioritizes metabolic features as potentially bioactive in a phenotype/condition of interest using a combination of (a) covariance with annotated metabolites, (b) ecological properties such as abundance with respect to covarying annotated compounds, and (c) differential abundance in the phenotype/condition of interest. If you have questions, please direct it to: [Macarron Forum](https://forum.biobakery.org/c/microbial-community-profiling/Macarron) # Installation Macarron requires R version 4.2.0 or higher. Install Bioconductor and then install Macarron: ```{r, eval=FALSE} if(!requireNamespace("BiocManager", quietly = TRUE)) install.packages("BiocManager") BiocManager::install("Macarron") ``` # Running Macarron Macarron can be run from the command line or as an R function. Both methods require the same arguments, have the same options, and use the same default settings. The package includes the wrapper `Macarron()` as well as functions which perform different steps in the Macarron framework. ## Input CSV files Macarron requires 4 comma-separated, appropriately formatted input files. The files and their formatting constraints are described below. 1. Metabolic features abundances * Must contain features in rows and samples in columns. * First column must identify features. 2. Metabolic features annotations * Must contain features in rows and annotations in columns. * First column must identify features. * Second column must contain either HMDB ID or PubChem Compound Identifier (CID). * Third column must contain the name of the metabolite. * Fourth column must contain a continuous chemical property such as m/z or RT or shift/ppm. * Other annotations such as RT, m/z or other identifiers can be listed column 4 onward. 3. Sample metadata * Must contain samples in rows and metadata in columns. * First column must identify samples. * Second column must contain categorical metadata relevant to prioritization such as phenotypes, exposures or environments. 4. Chemical taxonomy * First column must contain the HMDB ID or PubChem CID. IDs must be consistent between annotation and taxonomy files. * Second and third columns must contain chemical subclass and class of the respective metabolite. If you do not have the chemical taxonomy file, you can generate this file using the annotation dataframe and Macarron utility `decorate_ID` (see Advanced Topics). ## Output Files By default, all files will be stored in a folder named Macarron_output inside the current working directory. The main prioritization results are stored in ``prioritized_metabolites_all.csv``. Another file, ``prioritized_metabolites_characterizable.csv`` is a subset of ``prioritized_metabolites_all.csv`` and only contains metabolic features which covary with at least one annotated metabolite. The columns in these output files are: - Feature_index: Lists the identifier of the metabolic feature found in column 1 of abundance and annotation files. - HMDB_ID (or PubChem ID): Public database identifier from column 2 of annotation file (column 1 of annotation dataframe). - Metabolite name: From column 2 of annotation dataframe. - mz: The continuous numerical chemical property from column 3 of the annotation dataframe. - Priority_score: 1 indicates most prioritized. It is the percentile from the meta-rank of AVA, q-value and effect size. - Status: Direction of perturbation (differential abundance) in the phenotype (or environment) of interest compared to reference phenotype. - Module: ID of the covariance module a metabolic feature is a member of. Module = 0 indicates a singleton i.e., a metabolic feature that is not assigned to any module. - Anchor (of a module): Metabolic feature that has the highest abundance in any phenotype. - Related_classes: Chemical taxonomy of the annotated features that covary with a metabolic feature. - Covaries_with_standard: 1 (yes) and 0 (no). Column specifies if the metabolic feature covaries with at least one annotated (standard) metabolite. - AVA: Abundance versus anchor which is a ratio of the highest abundance (in any phenotype) of a metabolic feature and highest abundance of the covarying anchor. Naturally, the AVA of an anchor metabolite is 1. - qvalue: Estimated from multivariate linear model using `Maaslin2`. - effect_size - Remaining columns from the annotation dataframe are appended. ## Run a demo in R ### Using CSV files as inputs Example (demo) input files can be found under ``inst/extdata`` folder of the `Macarron` source. These files were generated from the [PRISM](https://pubmed.ncbi.nlm.nih.gov/30531976/) study of stool metabolomes of individuals with inflammatory bowel disease (IBD) and healthy "Control" individuals. Control and IBD are the two phenotypes in this example. Macarron will be applied to prioritize metabolic features with respect to their bioactivity in IBD. Therefore, in this example, the phenotype of interest is "IBD" and the reference phenotype is "Control". The four input files are ``demo_abundances.csv``, ``demo_annotations.csv``, ``demo_metadata.csv``, and ``demo_taxonomy.csv``. ```{r} library(Macarron) prism_abundances <- system.file( 'extdata','demo_abundances.csv', package="Macarron") prism_annotations <-system.file( 'extdata','demo_annotations.csv', package="Macarron") prism_metadata <-system.file( 'extdata','demo_metadata.csv', package="Macarron") mets_taxonomy <-system.file( 'extdata','demo_taxonomy.csv', package="Macarron") prism_prioritized <- Macarron::Macarron(input_abundances = prism_abundances, input_annotations = prism_annotations, input_metadata = prism_metadata, input_taxonomy = mets_taxonomy) ``` ### Using dataframes as inputs ```{r, eval=FALSE} abundances_df = read.csv(file = prism_abundances, row.names = 1) # setting features as rownames annotations_df = read.csv(file = prism_annotations, row.names = 1) # setting features as rownames metadata_df = read.csv(file = prism_metadata, row.names = 1) # setting samples as rownames taxonomy_df = read.csv(file = mets_taxonomy) # Running Macarron prism_prioritized <- Macarron::Macarron(input_abundances = abundances_df, input_annotations = annotations_df, input_metadata = metadata_df, input_taxonomy = taxonomy_df) ``` ### Running Macarron as individual functions The `Macarron::Macarron()` function is a wrapper for the Macarron framework. Users can also apply individual functions on the input dataframes to achieve same results as the wrapper with the added benefit of storing output from each function for other analyses. There are seven steps: ```{r,eval = FALSE} # Step 1: Storing input data in a summarized experiment object prism_mbx <- prepInput(input_abundances = abundances_df, input_annotations = annotations_df, input_metadata = metadata_df) # Step 2: Creating a distance matrix from pairwise correlations in abundances of metabolic features prism_w <- makeDisMat(se = prism_mbx) # Step 3: Finding covariance modules prism_modules <- findMacMod(se = prism_mbx, w = prism_w, input_taxonomy = taxonomy_df) # The output is a list containing two dataframes- module assignments and measures of success # if evaluateMOS=TRUE. To write modules to a separate dataframe, do: prism_module_assignments <- prism_modules[[1]] prism_modules_mos <- prism_modules[[2]] # Step 4: Calculating AVA prism_ava <- calAVA(se = prism_mbx, mod.assn = prism_modules) # Step 5: Calculating q-value prism_qval <- calQval(se = prism_mbx, mod.assn = prism_modules) # Step 6: Calculating effect size prism_es <- calES(se = prism_mbx, mac.qval = prism_qval) # Step 7: Prioritizing metabolic features prism_prioritized <- prioritize(se = prism_mbx, mod.assn = prism_modules, mac.ava = prism_ava, mac.qval = prism_qval, mac.es = prism_es) # The output is a list containing two dataframes- all prioritized metabolic features and # only characterizable metabolic features. all_prioritized <- prism_prioritized[[1]] char_prioritized <- prism_prioritized[[2]] # Step 8 (optional): View only the highly prioritized metabolic features in each module prism_highly_prioritized <- showBest(prism_prioritized) ``` Session info from running the demo in R can be displayed with the following command. ```{r} sessionInfo() ``` ## Advanced Topics ### Generating the input chemical taxonomy file The input taxonomy dataframe can be generated using the input metabolic features annotation dataframe using `Macarron::decorateID()`. This function annotates an HMDB ID or a PubChem CID with the chemical class and subclass of the metabolite. ```{r, eval=FALSE} taxonomy_df <- decorateID(input_annotations = annotations_df) write.csv(taxonomy_df, file="demo_taxonomy.csv", row.names = FALSE) ``` ### Accessory output files #### Macarron.log A record of all chosen parameters and steps that were followed during execution. #### modules_measures_of_success.csv This file provides information about the properties of covariance modules used in the analysis. By default, modules are generated using a minimum module size (MMS) (argument: `min_module_size`) equal to cube root of the total number of prevalent metabolic features. Macarron evaluates 9 measures of success (MOS) that collectively capture the "correctness" and chemical homogeneity of the modules. The MOS are as follows: - Total modules: Number of modules. - Singletons: Number of metabolic features that were not assigned to any module at MMS. - % Annotated modules: Percentage of modules that contained at least one annotated metabolic feature. - % Consistent assignments: Percentage of times the same metabolic feature was assigned to the same module e.g. if three metabolic features represent glucose, they should all be in the same module. This percentage must be high. - Max classes per module: The highest number of chemical classes observed in any module. This is evaluated using the chemical taxonomy of covarying annotated features. - 90p classes per module: 90th percentile of classes per module; captures the chemical homogeneity of the modules. - Max subclasses per module: The highest number of chemical subclasses observed in any module. - 90p subclasses per module: 90th percentile of subclasses per module; captures the chemical homogeneity of the modules. - % Features in HAM: Macarron first finds homogeneously annoted modules (HAMs): These are modules in which >75% annotated features have the same chemical class indicating that they are chemically homogeneous. It then calculates how many features the HAMs account for. #### Maaslin2 results This folder contains the Maaslin2 log file (maaslin2.log), significant associations found by Maaslin2 (significant_results.tsv) and the linear model residuals file (residuals.rds). For more information, see Maaslin2. ### Changing defaults #### Filtering metabolic features based on prevalence Ideally, at least 50% metabolic features must be retained after prevalence filtering. By default, Macarron uses the union of metabolic features observed (non-zero abundance) in at least 70% samples of any phenotype for further analysis. This prevalence threshold may be high for some metabolomics datasets and can be changed using the `min_prevalence` argument. ```{r, eval=FALSE} prism_prioritized <- Macarron::Macarron(input_abundances = abundances_df, input_annotations = annotations_df, input_metadata = metadata_df, input_taxonomy = taxonomy_df, min_prevalence = 0.5) # or prism_w <- makeDisMat(se = prism_mbx, min_prevalence = 0.5) ``` ### Minimum module size By default, cube root of the total number of prevalent features is used as the minimum module size (MMS) (argument: `min_module_size`) for module detection and generation. We expect this to work for most real world datasets. To determine if the modules are optimal for further analysis, Macarron evaluates several measures of success (MOS) as described above. In addition to evaluating MOS for modules generated using the default MMS, Macarron also evaluates MOS for MMS values that are larger (MMS+5, MMS+10) and smaller (MMS-5, MMS-10) than the default MMS. If you find that the MOS improve with larger or smaller MMS, you may change the default accordingly. For more details about module detection, please see `WGCNA` and `dynamicTreeCut`. ```{r, eval=FALSE} # See MOS of modules generated using default prism_modules <- findMacMod(se = prism_mbx, w = prism_w, input_taxonomy = taxonomy_df) prism_modules_mos <- prism_modules[[2]] View(prism_modules_mos) # Change MMS prism_modules <- findMacMod(se = prism_mbx, w = prism_w, input_taxonomy = taxonomy_df, min_module_size = 10) ``` ### Specifying fixed effects, random effects and reference Macarron uses Maaslin2 for determining the q-value of differential abundance in a phenotype of interest. For default execution, the phenotype of interest must be a category in column 1 of the metadata dataframe e.g. IBD in diagnosis in the demo. This is also the column that is picked by the `metadata_variable` argument for identifying the main phenotypes/conditions in any dataset (see Macarron.log file). Further, in the default execution, all columns in the metadata table are considered as fixed effects and the alphabetically first categorical variable in each covariate with two categories is considered as the reference. Maaslin2 requires reference categories to be explicitly defined for all categorical metadata with more than two categories. Defaults can be changed with the arguments `fixed_effects`, `random_effects` and `reference`. In the demo example, `fixed effects` and `reference` can be defined as follows: ```{r, eval=FALSE} prism_qval <- calQval(se = prism_mbx, mod.assn = prism_modules, metadata_variable = "diagnosis", fixed_effects = c("diagnosis","age","antibiotics"), reference = c("diagnosis,Control";"antibiotics,No")) ``` # Command line invocation The package source contains a script `MacarronCMD.R` in `inst/scripts` to invoke Macarron in the command line using Rscript. The `inst/scripts` folder also contains a README file that comprehensively documents the usage of the script.